EZGRT-89K | Human Ghrelin (total) ELISA

EZGRT-89K
96-well strip plate  
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      Overview

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      Ghrelin (total)HELISAColorimetric, Fluorescent
      Description
      Catalogue Number EZGRT-89K
      Description Human Ghrelin (total) ELISA
      Background Information Circulating ghrelin is a multifunctional hormone secreted primarily by the stomach.
      References
      Product Information
      Detection method Colorimetric Fluorescent
      Specificity Human Ghrelin (active) 80% Des-Octanoyl Human Ghrelin 100% Canine Ghrelin (active) 70% Porcine Ghrelin (active) not detectable Motilin Related Peptide (Human, Rat/Mouse) not detectable PYY 3-36 (Human, Mouse, Porcine) not detectable NPY (Human/Rat)
      Precision, %
      • Intra-assay: Serum: 0.9–1.3% Plasma: 1.1–1.9%
      • Inter-assay: Serum: 6.2–7.8% Plasma: 5.2–7.7%
      Applications
      Application This Human Ghrelin (total) ELISA is used to measure & quantify Ghrelin (total) levels in Metabolism & Endocrine & Neuroscience research.
      Key Applications
      • ELISA
      Application Notes Room temperature, 3 hour assay, 20 μL sample size (serum or plasma)
      Biological Information
      Specificity Human Ghrelin (active) 80% Des-Octanoyl Human Ghrelin 100% Canine Ghrelin (active) 70% Porcine Ghrelin (active) not detectable Motilin Related Peptide (Human, Rat/Mouse) not detectable PYY 3-36 (Human, Mouse, Porcine) not detectable NPY (Human/Rat)
      Species Reactivity Human
      Analytes Available
      • Ghrelin (total)
      Entrez Gene Number
      Entrez Gene Summary Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor (GHSR; MIM 601898) and is involved in regulating growth hormone (GH; MIM 139250) release. Ghrelin is derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 (MIM 602886) and is involved in satiety and decreased food intake.[supplied by OMIM]
      Gene Symbol
      • MTLRP
      • GHRL
      • ghrelin
      • obestatin
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: Q9UBU3 # Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility (By similarity).
      SIZE: 117 amino acids; 12911 Da
      SUBCELLULAR LOCATION: Secreted.
      TISSUE SPECIFICITY: Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
      PTM: O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28- C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28- C10:1, may be modified with a singly unsaturated carboxylic acid. & Amidation of Leu-98 is essential for obestatin activity (By similarity).
      SIMILARITY: SwissProt: Q9UBU3 ## Belongs to the motilin family.
      Accuracy
      • (Des-octanoyl Ghrelin) 112–119%
      • Serum: (Active Ghrelin) 88–91%
      • (Des-octanoyl Ghrelin) 101–107%
      • Plasma: (Active Ghrelin) 84–87%
      Physicochemical Information
      Sensitivity
      • 30 pg/mL (20 µL sample size)
      Linearity of Dilution Serum: 95.7–104.7% Plasma: 95–117%
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Sales Restrictions This product is not available for sale in Japan.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size 96-well strip plate
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      HUMAN TOTAL GHRELIN ELISA KIT

      MSDS

      Languages
      English

      References

      Reference overviewPub Med ID
      Changes in glucose homeostasis after Roux-en-Y gastric bypass surgery for obesity at day three, two months, and one year after surgery: role of gut peptides.
      Falkén Y, Hellström PM, Holst JJ, Näslund E
      The Journal of clinical endocrinology and metabolism 96 2227-35. Epub 2011 May 4. 2011

      21543426
      The fermentable fibre inulin increases postprandial serum short-chain fatty acids and reduces free-fatty acids and ghrelin in healthy subjects.
      Tarini J, Wolever TM
      Applied physiology, nutrition, and metabolism = Physiologie 35 9-16. 2010

      Show Abstract
      20130660
      Alteration in chromogranin A, obestatin and total ghrelin levels of saliva and serum in epilepsy cases.
      Ersel Dag,Suleyman Aydin,Yusuf Ozkan,Fazilet Erman,Adile Ferda Dagli,Mehtap Gurger
      Peptides 31 2010

      Show Abstract
      20172008

      Brochure

      Title
      Immunoassay & Multiplex Kits: Analyte Quarterly, Volume 2, 2014

      Technical Info

      Title
      Quality Control Ranges: HUMAN TOTAL GHRELIN ELISA KIT - 96-Well Plate