Key Spec Table

Key ApplicationsSpeciesPurity
CultMouseThe active component mLIF has been shown to be >95% pure by SDS-PAGE. ESGRO® Supplement is supplied 0.22 micron sterile filtered, and tested negative in mycoplasma tests.
Description
Catalogue Number ESG1107
Brand Family Chemicon®
Synonyms Murine LIF
Trade Name
  • Chemicon
Description ESGRO® Leukemia Inhibitory Factor (LIF), 10 million units/1 mL
References
Product Information
Format Purified
Presentation ESGRO® Supplement is supplied in liquid form as 107 Units in 1.0 mL of phosphate buffered saline with 1% w/v bovine serum albumin BSA as a carrier for stability.
Applications
Application The ESGRO Leukemia Inhibitory Factor (LIF) for use as a reagent for the in vitro maintenance of the pluripotential phenotype of murine ES cells. It includes 10^7 units.
Key Applications
  • Stem Cell Culture
Application Notes Applications for ESGRO® Supplement include use as a reagent for the in vitro maintenance of the pluripotential phenotype of murine ES cells.

SUGGESTED PROTOCOLS:

ES Cells: In D3 and MBL-1 pluripotential ES cell cultures it is routinely found that 1000 U ESGRO® Supplement per 1.0 mL of tissue culture media is required to maintain ES cells with a stem cell phenotype. Similar concentrations of mLIF have also been used for germline transmission of genetically altered ES cells(E3).

At the recommended concentration 107 units of ESGRO® is sufficient for 10.0 L of tissue culture media and 106 units of ESGRO® Supplement is sufficient for 1.0 L of tissue culture media.
Biological Information
Concentration 10 million units/1 mL
Purity The active component mLIF has been shown to be >95% pure by SDS-PAGE. ESGRO® Supplement is supplied 0.22 micron sterile filtered, and tested negative in mycoplasma tests.
Species Mouse
Species Reactivity Mouse
Specific Activity ESGRO® Supplement is assessed both on mouse embryonic stem cells (D3 & MBL-1) and on murine M1 myeloid leukemic cells(4). A standard of 50 Units is defined as the concentration of ESGRO® Supplement, in 1.0mL of tissue culture medium, that induces the differentiation of 50% of M1 colonies(4). 10E7 units is equivalent to approximately 100 μg of pure protein, and is sufficient to treat 10L of ES cell culture media. <br /><br />Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mL Murine myeloid leukemic, M1 Assay: Specific Activity >/= 10<sup>8</sup> units/mg
Entrez Gene Number
Entrez Gene Summary Leukaemia inhibitory factor is a cytokine that induces macrophage differentiation. Neurotransmitters and neuropeptides, well known for their role in the communication between neurons, are also capable of activating monocytes and macrophages and inducing chemotaxis in immune cells. LIF signals through different receptors and transcription factors. LIF in conjunction with BMP2 acts in synergy on primary fetal neural progenitor cells to induce astrocytes.
Gene Symbol
  • D-FACTOR
  • LIF
  • Emfilermin
  • CDF
  • MLPLI
  • HILDA
UniProt Number
UniProt Summary FUNCTION: SwissProt: P15018 # LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
SIZE: 202 amino acids; 22008 Da
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: SwissProt: P15018 ## Belongs to the LIF/OSM family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • LABEL LICENSE - USE RESTRICTED TO RESEARCH USE ONLY

    Leukemia Inhibitory Factor ("LIF") is protected by US Patents No. 5,443,825 and 5,750,654 and Canada Patent No. 1,341,581 and 1,341,469 owned by AMRAD Corporation Ltd. and licensed exclusively to Millipore for research uses. The use of this product is limited for research uses only and not for any commercial purposes, such as making or developing other research products from this product or use of this product as a component in a research kit. This product is not to be used in humans or for any diagnostic purposes.

    The purchaser may refuse to accept the conditions of this label license by returning this product unopened and will receive a full refund of the purchase price paid.

    For-profit entities or commercial research entities purchasing this product shall be required to execute a separate commercial license with Millipore within three months of purchase. Researchers may not transfer this product or its derivatives to researchers performing commercial research at other Nonprofit Organizations, or to For-Profit Organizations, without the express written consent of Millipore, and without those entities having an express license from Millipore for the use of the product. Other than as specifically provided herein, a transferee of this product shall have no right to transfer this product, or its derivatives to any other person or entity.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions ESGRO® Supplement is shipped at ambient temperature. A cold pack may be included for customer relations, however it is truly not needed. Extensive stability tests have been performed with ESGRO® Supplement during development. This product is stable for at least 18 months from the date of manufacture, in the concentrated form or diluted in sterile tissue culture media, with no loss of activity on ES cells. For long term storage it is recommended that ESGRO® Supplement be stored at 4°C. Freeze-thawing will reduce potency. It is recommended that prior to use, ESGRO® Supplement should be diluted in sterile tissue culture media and aliquoted to a convenient concentration, then stored at 4°C. Freeze-thawing should be avoided. Product is stable for a minimum of 7 days at 37°C, 5% CO2 incubator during the culture of ES cells.
Packaging Information
Material Size 107 units
Material Package 1 mL
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
General Experimental Outline for Targeting ES Cells

MSDS

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Certificates of Analysis

TitleLot Number
ESGRO#174; Mouse LIF Medium Supplement - 2073738 2073738
ESGRO#174; Mouse LIF Medium Supplement - 2011556 2011556
ESGRO#174; Mouse LIF Medium Supplement - 2025027 2025027
ESGRO#174; Mouse LIF Medium Supplement - 2032261 2032261
ESGRO#174; Mouse LIF Medium Supplement - 2052074 2052074
ESGRO#174; Mouse LIF Medium Supplement - 2152237 2152237
ESGRO#174; Mouse LIF Medium Supplement - 2179114 2179114
ESGRO#174; Mouse LIF Medium Supplement - 2197552 2197552
ESGRO#174; Mouse LIF Medium Supplement - NG1951660 NG1951660
ESGRO#174; Supplement (107 Units) - DAM1739361 DAM1739361
ESGRO#174; Supplement (107 Units) - DAM1770436A DAM1770436A
ESGRO#174; Supplement (107 Units) - DAM1835453 DAM1835453
ESGRO#174; Supplement (107 Units) - JBC1864978 JBC1864978
ESGRO#174; Supplement (107 Units) - JBC1872217 JBC1872217
ESGRO#174; Supplement (107 Units) - JBC1908448 JBC1908448
ESGRO#174; Supplement (107 Units) - NG1652954 NG1652954
ESGRO#174; Supplement (107 Units) - NG1654682 NG1654682
ESGRO#174; Supplement (107 Units) - NG1667894 NG1667894
ESGRO#174; Supplement - DAM1776540 DAM1776540
ESGRO#174; Supplement - DAM1821253 DAM1821253
ESGRO® Mouse LIF Medium Supplement -2461168 2461168
MURINE ESGRO 107 - ME9EN5057 ME9EN5057
MURINE ESGRO 107 - ME9HN2210 ME9HN2210

References | 14 Available | See All References

Reference overviewPub Med ID
A kinase inhibitor screen identifies small-molecule enhancers of reprogramming and iPS cell generation.
Li, Zhonghan and Rana, Tariq M
Nat Commun, 3: 1085 (2012) 2012

Show Abstract
23011139
A conditional knockout resource for the genome-wide study of mouse gene function
Skarnes W. C. et al.
Nature 474(7351) 337-342 2011

Show Abstract
21677750
Dynamic regulation of 5-hydroxymethylcytosine in mouse ES cells and during differentiation.
Ficz G. et al.
Nature 473(7347) 398-402 2011

Show Abstract
21460836
Proliferating versus differentiating stem and cancer cells exhibit distinct midbody-release behaviour.
Ettinger A. W. et al.
Nat. Commun. 2 503 2011

Show Abstract
22009035
Optimization of Protocols for Derivation of Mouse Embryonic Stem Cell Lines from Refractory Strains, Including the Non Obese Diabetic Mouse.
Davies T. J. & Fairchild P. J.
Stem Cells Dev. Nov. 2011

Show Abstract
21933027
Isolation and characterization of neural crest-derived stem cells from dental pulp of neonatal mice.
Janebodin, Kajohnkiart, et al.
PLoS ONE, 6: e27526 (2011) 2011

Show Abstract
22087335
Novel hyperactive transposons for genetic modification of induced pluripotent and adult stem cells: a nonviral paradigm for coaxed differentiation.
Belay, Eyayu, et al.
Stem Cells, 28: 1760-71 (2010) 2010

Show Abstract
20715185
Using small molecules to improve generation of induced pluripotent stem cells from somatic cells
Desponts C. & Ding S.
Methods Mol. Biol. 636 207-218 2010

Show Abstract
20336525
Short RNAs are transcribed from repressed polycomb target genes and interact with polycomb repressive complex-2.
Kanhere, Aditi, et al.
Mol. Cell, 38: 675-88 (2010) 2010

Show Abstract
20542000
Optimized mouse ES cell culture system by suspension growth in a fully defined medium.
Andäng M. et al.
Nat. Protoc, 3(6) 1013-1017 2008

Show Abstract
18536648

Brochure

Title
Murine Embryonic Stem Cell Culture Procedures & Protocols

Technical Info

Title
Stem Cell Testing of PES Membrane Containing Stericup Filters

Data Sheet

Title
Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation
STEMCCA Lentivirus Reprogramming Kits

FAQ

QuestionAnswer
Why are my mouse embryonic stem cells differentiating and what preventative measures do you recommend?Recommendation: Regular use of Penicillin / Streptomycin in media can often mask a low level contamination with agents such as mycoplasma. It is recommended to have your cell lines tested for mycoplasma on a periodic basis.
Cause of Differation: Incubator settingsRecommendation: Ensure that the CO2 incubator readings are correct at 37oC and 5% CO2.
Cause of Differation: Concentration of ES cellsRecommendation: Low confluency of ES cells can result in differentiation. ES cells should be plated at a minimum density of 1x106 cells / 100mm dish. Refer to the attached figures for illustrations of ES cell confluency. Refer to figs. 1, 2, 3, 4, and 5.
Cause of Differation: GelatinRecommendation: It is preferable to use cell culture grade gelatin at all times (even if using feeder layers) as the gelatin minimises surface differences on the tissue culture plates.
Cause for the lack of chimeras: ES cell passage numberRecommendation: In general; the best chimeras are generated from low passage number ES cells. If cell lines have been cultured for a high number of passages, it is recommended to go back to a lower passage frozen stock.
Cause for the lack of chimeras: Time taken prior to microinjectionRecommendation: It is recommend that ES cells be microinjected as soon as possible after removing them from the tissue culture plates, and not left on ice or room temperature for extended periods.
Why are my mouse embryonic stem cells differentiating and what preventative measures do you recommend?Recommendation: Regular use of Penicillin / Streptomycin in media can often mask a low level contamination with agents such as mycoplasma. It is recommended to have your cell lines tested for mycoplasma on a periodic basis.
Why are my mouse embryonic stem cells differentiating and what preventative measures do you recommend?Recommendation: Regular use of Penicillin / Streptomycin in media can often mask a low level contamination with agents such as mycoplasma. It is recommended to have your cell lines tested for mycoplasma on a periodic basis.

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