ECM541 | ECM Cell Culture Optimization Array (Colorimetric, 96-Wells)

ECM541
1 kit  1 plate, 96-wells
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      Overview

      Key Spec Table

      Detection Methods
      Colorimetric
      Description
      Catalogue Number ECM541
      Description ECM Cell Culture Optimization Array (Colorimetric, 96-Wells)
      Overview Extracellular matrix (ECM) proteins are produced intracellularly and are subsequently secreted into the surrounding cellular medium, actively regulating a diverse range of cell functions including cell adhesion, differentiation, proliferation, migration, invasion and survival. A primary utility of ECMs in in vitro culture is to promote cellular adhesion while maintaining cell viability and maximizing cell proliferation for downstream cell-based applications. In cases where optimal cell growth conditions are not well defined and critical adhesion protein requirements are unknown, identifying the ideal ECM protein(s) at functionally relevant concentration(s) can be a time-consuming and labor-intensive process. The 96-well, colorimetric ECM Cell Culture Optimization Array provides a multiparametric assay that allows researchers to: (1) quickly identify ECM(s) that best promote cell-type specific adhesion and (2) pinpoint the optimal ECM concentration required to promote maximum adhesion for a particular cell type.

      How does the ECM Cell Culture Optimization Array work?
      The foundation of our kit is a 96-well microtiter plate containing several commonly-used ECM proteins. Collagen I, laminin, fibronectin, and vitronectin are arrayed in triplicate with concentrations ranging from 0.125 μg/mL to 20 μg/mL. You simply culture your cells on the plate, and use the included reagents to block, stain, and analyze the adhesion capacity with a plate reader. We have extensively tested and optimized this kit on a variety of cell types including HEK293 cells, and human and rodent neural stem cells.
      References
      Product Information
      Components
      • • ECM Gradient Array Plate: One 96-well plate with 12 strips
      • • Assay Buffer: (Part No. 2003646) One bottle - 100 mL
      • • Extraction Buffer: (Part No. 90145) One bottle - 20 mL
      • • Cell Stain Solution: (Part No. 90144) One bottle - 20 mL.
      • • 30%BSA: (Part No. CS201638) One vial - 0.5mL
      Detection method Colorimetric
      Applications
      Application The ECM cell culture Optimization Array enables researchers to identify the best ECM protein for their cell culture environment & also pinpoint the concentration needed to achieve optimal cell growth conditions.
      Application Notes The ECM Cell Culture Optimization Array is the first commercially-available tool of its kind to enable researchers to not only quickly identify the best ECM protein for their cell culture environment, but also pinpoint the concentration needed to achieve optimal cell growth conditions.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions The ECM Gradient Array Plate should be stored in the foil pouch at -70ºC, and used within three months of the date of receipt. The remaining kit components should be stored at 2º to 8ºC.
      Packaging Information
      Material Size 1 kit
      Material Package 1 plate, 96-wells
      Transport Information
      Supplemental Information
      Specifications

      Related Products & Applications

      Alternative Format

       Catalogue Number Description  
      ECM542 ECM Cell Culture Optimization Array (Colorimetric, 48-Wells) Show Pricing & Availability
      ECM546 ECM Cell Culture Optimization Array (Fluorometric, 96-Wells) Show Pricing & Availability

      Kit Component

       Catalogue Number Description  
      CC050 Human Collagen Type I Show Pricing & Availability
      CC080 Vitronectin, Human Purified Protein Show Pricing & Availability
      CC095 Laminin, mouse purified Show Pricing & Availability
      FC010 Human Plasma Fibronectin Purified Protein Show Pricing & Availability

      Product Families

      ECM Proteins and Coated Plates

      Meet cell type-specific needs for growth and adhesion Learn More >>

      Categories

      Life Science Research > Cell Analysis > Cell-based Assays > Cell Adhesion Assays