CYT306 | ChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA

Graph of BDNF Standard Curve
CYT306
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      Overview

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      BDNFR, HELISAChromogenic
      Description
      Catalogue Number CYT306
      Brand Family Chemicon®
      Synonyms BDNF
      Trade Name
      • Chemicon
      • ChemiKine
      Description ChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA
      Overview ChemiKine™ Brain Derived Neurotrophic Factor (BDNF) kit is a sandwich enzyme immunoassay (EIA), which measures BDNF. The kit will measure BDNF from human and rat.

      Test Principle:

      With the ChemiKine BDNF assay system, rabbit polyclonal antibodies generated against human BDNF are coated onto a microplate and are used to capture BDNF from a sample. BDNF specific, biotin conjugated, mouse monoclonal antibodies detect the captured BDNF. After addition of streptavidin-enzyme, substrate and stop solution the amount of BDNF is determined. The standard curve demonstrates a direct relationship between Optical Density (OD) and BDNF concentration: i.e., the higher the OD the higher the BDNF concentration in the sample.

      Application:

      ChemiKine BDNF kit is designed to measure the amount of BDNF in cell culture supernatants, tissue homogenates and biological fluid (serum, plasma, and serum-free) samples from human and rat. There are enough reagents included in this kit for two 96-well immuno-assay plates. Running duplicate wells for samples and standards is recommended.

      For Research Use Only. Not for use in diagnostic procedures.
      References
      Product Information
      Components Stop Solution (Part No. 60260): One 22 mL bottle of an HCl solution. ChemiKine BDNF ELISA Plate: (Part No. 60238) Two Pre-coated with Rabbit anti-Human BDNF Polyclonal Antibody 96-Well Immunoplates sealed in foil pouches. Wash Buffer Concentrate: (Part No. 60245) One 100 mL (10X) bottle of Concentrate. Standard/Sample Diluent: (Part No. 60240) One 60 mL bottle (Ready to Use). BDNF Standard (Recombinant Human): (Part No. 60237) Two vials (Lyophilized). Biotinylated Mouse anti-Human BDNF Monoclonal Antibody: (Part No. 60583) One 25 μL vial. Streptavidin-Enzyme conjugate: (Part No. 60582) One 50 mL vial of HRP conjugated Streptavidin. TMB/E Solution: (Part No. 60096) Two 10 mL bottles of a Ready to Use solution of 3,3',5,5'-tetramethylbenzidine in a proprietary buffer with enhancer.
      Detection method Chromogenic
      Applications
      Application This ChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA is used to measure & quantify BDNF levels in Neuroscience research.
      Key Applications
      • ELISA
      Biological Information
      Species Reactivity Rat Human
      Analytes Available
      • BDNF
      Entrez Gene Number
      Entrez Gene Summary The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene, but the full-length nature of only some could be determined.
      Gene Symbol
      • MGC34632
      • BDNF
      • Abrineurin
      • neurotrophin
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: P23560 # Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
      SIZE: 247 amino acids; 27818 Da
      SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB.
      SUBCELLULAR LOCATION: Secreted.
      PTM: The propeptide is N-glycosylated and glycosulfated. & Converted into mature BDNF by plasmin (PLG) (By similarity).
      DISEASE: SwissProt: P23560 # Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases.
      SIMILARITY: SwissProt: P23560 ## Belongs to the NGF-beta family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Precautions:

      · Wash Buffer and Sample Buffer contain thimerosal. Thimerosal is highly toxic by inhalation, contact with skin or if swallowed. Thimerosal is a possible mutagen and should be handled accordingly.

      · The instructions provided have been designed to optimize the kit's performance. Deviation from the instructions may result in suboptimal performance of the kit and the failure to produce accurate data.
      Packaging Information
      Material Size 1 kit
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Languages
      English

      References | 24 Available | See All References

      Reference overviewSpeciesPub Med ID
      Expression and localization of brain-derived neurotrophic factor (BDNF) mRNA and protein in human submandibular gland.
      Juri Saruta,Kazuhiro Fujino,Masahiro To,Keiichi Tsukinoki
      Acta histochemica et cytochemica 45 2012

      Show Abstract
      23012486
      Various levels of plasma brain-derived neurotrophic factor in patients with tinnitus.
      Fumiyuki Goto,Juri Saruta,Sho Kanzaki,Masahiro To,Tomoko Tsutsumi,Keiichi Tsukinoki,Kaoru Ogawa
      Neuroscience letters 510 2012

      Show Abstract
      22281446
      Decreased levels of brain-derived neurotrophic factor in the remitted state of unipolar depressive disorder.
      B J Hasselbalch,U Knorr,B Bennike,S G Hasselbalch,M H Greisen Søndergaard,L Vedel Kessing
      Acta psychiatrica Scandinavica 126 2012

      Show Abstract
      22292883
      BDNF as an effect modifier for gender effects on pain thresholds in healthy subjects.
      Luciana Cadore Stefani,Iraci Lucena da Silva Torres,Izabel Cristina Custodio de Souza,Joanna Ripoll Rozisky,Felipe Fregni,Wolnei Caumo
      Neuroscience letters 514 2012

      Show Abstract
      22395087
      Propofol interacts with stimulus intensities of electroconvulsive shock to regulate behavior and hippocampal BDNF in a rat model of depression.
      Jie Luo,Su Min,Ke Wei,Junfang Zhang,Yuanyuan Liu
      Psychiatry research 198 2012

      Show Abstract
      22410590
      Prolonged therapeutic hypothermia does not adversely impact neuroplasticity after global ischemia in rats.
      Gergely Silasi,Ana C Klahr,Mark J Hackett,Angela M Auriat,Helen Nichol,Frederick Colbourne
      Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 32 2012

      Show Abstract
      22434072
      Serum MIP-1 alpha level: a biomarker for the follow-up of lentiviral therapy in mucopolysaccharidosis IIIB mice.
      Paola Di Natale,Carmela Di Domenico,Daniele Di Napoli
      Journal of inherited metabolic disease 33 2010

      Show Abstract
      20162365
      Increase in brain-derived neurotrophic factor in first episode psychotic patients after treatment with atypical antipsychotics.
      González-Pintoa A, Mosquera F, Palomino A, Alberich S, Gutiérrez A, Haidar K, Vega P, Barbeito S, Ortiz A, Matute C
      Int Clin Psychopharmacol 25 241-5. 2010

      Show Abstract
      20568658
      IFATS collection: The conditioned media of adipose stromal cells protect against hypoxia-ischemia-induced brain damage in neonatal rats.
      Xing Wei, Zhimei Du, Liming Zhao, Dongni Feng, Gang Wei, Yongzheng He, Jiangning Tan, Wei-Hui Lee, Harald Hampel, Richard Dodel, Brian H Johnstone, Keith L March, Martin R Farlow, Yansheng Du
      Stem cells (Dayton, Ohio) 27 478-88 2009

      Show Abstract
      19023032
      The differential regulation of BDNF and TrkB levels in juvenile rats after four days of escitalopram and desipramine treatment.
      Megan E Kozisek, David Middlemas, David B Bylund
      Neuropharmacology 54 251-7 2008

      Show Abstract
      Rat18048068

      Brochure

      Title
      Immunoassay & Multiplex Kits: Analyte Quarterly, Volume 2, 2014

      Product Insert

      Title
      ChemiKineTM Brain Derived Neurotrophic Factor (BDNF) Sandwich ELISA Kit