CYL3039 | PrecisION™ hERG-HEK Recombinant Stable Cell Line

CYL3039
2 mL  2 x 1 ml aliquots
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      Overview

      Key Spec Table

      Application NotesSpeciesHost CellsProtein Target
      HEK293 cells expressing hERG were characterised in terms of their pharmacological and biophysical properties using whole-cell patch clamp techniques.

      The cell-line was shown to be selectively expressing hERG current since the isochronal and fully activated I/V plots were the same as those reported in the literature. Furthermore, tail currents were significantly blocked by low nM concentrations of astemizole and cisapride. Functional channel expression over time was monitored using IonWorks™ HT. Channel expression is robust over at least 30 passages: 92% of cells expressed hERG currents at passage 30 giving a mean tail current amplitude at -50 mV of 880 ± 20 pA (n=315).
      Human293 cell linehERG
      Description
      Catalogue Number CYL3039
      Brand Family Upstate
      Synonyms ERG KCNH2 ERG1 LQT2 erg1 HERG SQT1 H-ERG Kv11.1 HERG1 Erg1
      Trade Name
      • Upstate
      • PrecisION
      Description PrecisION™ hERG-HEK Recombinant Stable Cell Line
      Overview Recombinant HEK293 cell line expressing the human ERG (ether-a-go-go related gene) potassium channel.

      Format:
      2 x 1 ml aliquots containing 1.68 x 106cells/ml in 10% DMSO at passage 11.
      Background Information The FDA, ICH S7B guidelines set out a non-clinical testing strategy to assess the effects of pharmaceuticals on ventricular repolarisation and proarrhythmic risk. The most common mechanism for this is inhibition of the delayed rectified potassium current IKr. The human ether-à-go-go-related gene (hERG) encodes the potassium ion channel responsible for IKr.
      References
      Product Information
      Components
      • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial
      Presentation 2 x 1 ml aliquots
      Applications
      Application Human ERG ion channel stably expressed in HEK cells for use in electrophysiological assays.
      Application Notes HEK293 cells expressing hERG were characterised in terms of their pharmacological and biophysical properties using whole-cell patch clamp techniques.

      The cell-line was shown to be selectively expressing hERG current since the isochronal and fully activated I/V plots were the same as those reported in the literature. Furthermore, tail currents were significantly blocked by low nM concentrations of astemizole and cisapride. Functional channel expression over time was monitored using IonWorks™ HT. Channel expression is robust over at least 30 passages: 92% of cells expressed hERG currents at passage 30 giving a mean tail current amplitude at -50 mV of 880 ± 20 pA (n=315).
      Biological Information
      Species Human
      Entrez Gene Number
      Entrez Gene Summary This gene encodes a voltage-activated potassium channel belonging to the eag family. It shares sequence similarity with the Drosophila ether-a-go-go (eag) gene. Mutations in this gene can cause long QT syndrome type 2 (LQT2). Transcript variants encoding distinct isoforms have been identified.
      Gene Symbol
      • ERG
      • KCNH2
      • ERG1
      • LQT2
      • erg1
      • HERG
      • SQT1
      • H-ERG
      • Kv11.1
      • HERG1
      • Erg1
      Host Cells 293 cell line
      Protein Target hERG
      Target Sub-Family Potassium
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: Q12809 # Pore-forming (alpha) subunit of voltage-gated inwardly rectifying potassium channel. Channel properties are modulated by cAMP and subunit assembly. Mediates the rapidly activating component of the delayed rectifying potassium current in heart (IKr). Isoform 3 has no channel activity by itself, but modulates channel characteristics when associated with isoform 1.
      SIZE: 1159 amino acids; 126655 Da
      SUBUNIT: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits. Heteromultimer with KCNH6/ERG2 and KCNH7/ERG3. Interacts with ALG10B (By similarity). Heteromultimer with KCNE1 and KCNE2.
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Highly expressed in heart and brain.
      DOMAIN:SwissProt: Q12809 The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
      PTM: Phosphorylated on serine and threonine residues. Phosphorylation by PKA inhibits ion conduction.
      DISEASE: SwissProt: Q12809 # Defects in KCNH2 are the cause of long QT syndrome type 2 (LQT2) [MIM:152427]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to exercise or emotional stress. Deafness is often associated with LQT2. & Defects in KCNH2 are the cause of short QT syndrome type 1 (SQT1) [MIM:609620]. Short QT syndromes are heart disorders characterized by idiopathic persistently and uniformly short QT interval on ECG in the absence of structural heart disease in affected individuals. They cause syncope and sudden death.
      SIMILARITY: Belongs to the potassium channel family. H (Eag) subfamily. & Contains 1 cyclic nucleotide-binding domain. & Contains 1 PAC (PAS-associated C-terminal) domain. & Contains 1 PAS (PER-ARNT-SIM) domain.
      Cell Line Type
      • Ion Channel Cell Lines
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Mycoplasma Testing:
      The cell line has been screened to confirm the absence of Mycoplasma species
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size 2 mL
      Material Package 2 x 1 ml aliquots
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Cell Culture and Systems > Cell Lines > Ion Channel Cell Lines
      Life Science Research > Drug Discovery and Development > Ion Channel Cell Lines & Assays > Ion Channel Cell Lines