CYL3039 | PrecisION™ hERG-HEK Recombinant Stable Cell Line

CYL3039
2 mL  2 x 1 ml aliquots
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      Overview

      Replacement Information

      Key Spec Table

      Application Notes Species Host Cells Protein Target
      HEK293 cells expressing hERG were characterised in terms of their pharmacological and biophysical properties using whole-cell patch clamp techniques.

      The cell-line was shown to be selectively expressing hERG current since the isochronal and fully activated I/V plots were the same as those reported in the literature. Furthermore, tail currents were significantly blocked by low nM concentrations of astemizole and cisapride. Functional channel expression over time was monitored using IonWorks™ HT. Channel expression is robust over at least 30 passages: 92% of cells expressed hERG currents at passage 30 giving a mean tail current amplitude at -50 mV of 880 ± 20 pA (n=315).
      Human293 cell linehERG
      Description
      Catalogue Number CYL3039
      Brand Family Upstate
      Trade Name
      • PrecisION
      • Upstate
      Description PrecisION™ hERG-HEK Recombinant Stable Cell Line
      Overview Recombinant HEK293 cell line expressing the human ERG (ether-a-go-go related gene) potassium channel.

      Format:
      2 x 1 ml aliquots containing 1.68 x 106cells/ml in 10% DMSO at passage 11.
      Alternate Names
      • KCNH2
      • ERG
      • Erg1
      • HERG1
      • Kv11.1
      • H-ERG
      • SQT1
      • HERG
      • erg1
      • LQT2
      • ERG1
      Background Information The FDA, ICH S7B guidelines set out a non-clinical testing strategy to assess the effects of pharmaceuticals on ventricular repolarisation and proarrhythmic risk. The most common mechanism for this is inhibition of the delayed rectified potassium current IKr. The human ether-à-go-go-related gene (hERG) encodes the potassium ion channel responsible for IKr.
      References
      Product Information
      Components
      • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial
      Presentation 2 x 1 ml aliquots
      Applications
      Application Human ERG ion channel stably expressed in HEK cells for use in electrophysiological assays.
      Application Notes HEK293 cells expressing hERG were characterised in terms of their pharmacological and biophysical properties using whole-cell patch clamp techniques.

      The cell-line was shown to be selectively expressing hERG current since the isochronal and fully activated I/V plots were the same as those reported in the literature. Furthermore, tail currents were significantly blocked by low nM concentrations of astemizole and cisapride. Functional channel expression over time was monitored using IonWorks™ HT. Channel expression is robust over at least 30 passages: 92% of cells expressed hERG currents at passage 30 giving a mean tail current amplitude at -50 mV of 880 ± 20 pA (n=315).
      Biological Information
      Species Human
      Entrez Gene Number
      Entrez Gene Summary This gene encodes a voltage-activated potassium channel belonging to the eag family. It shares sequence similarity with the Drosophila ether-a-go-go (eag) gene. Mutations in this gene can cause long QT syndrome type 2 (LQT2). Transcript variants encoding distinct isoforms have been identified.
      Gene Symbol
      • KCNH2
      • ERG
      • Erg1
      • HERG1
      • Kv11.1
      • H-ERG
      • SQT1
      • HERG
      • erg1
      • LQT2
      • ERG1
      Host Cells 293 cell line
      Protein Target hERG
      Target Sub-Family Potassium
      Cell Line Type
      • Ion Channel Cell Lines
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Mycoplasma Testing:
      The cell line has been screened to confirm the absence of Mycoplasma species
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size 2 mL
      Material Package 2 x 1 ml aliquots
      Transport Information
      Supplemental Information
      Specifications

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      Alternative Format

       Catalogue Number Description  
      CYL3038 PrecisION hERG-CHO Recombinant Cell Line Show Pricing & Availability

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      Categories

      Life Science Research > Cell Culture and Systems > Cell Lines > Ion Channel Cell Lines
      Life Science Research > Drug Discovery and Development > Ion Channel Cell Lines & Assays > Ion Channel Cell Lines