CC080 | Vitronectin, Human Purified Protein

Vitronectin is a cell adhesion and spreading protein found in the ECM. Vitronectin interacts with cells through a number of integrin receptors containing the alphaV subunit. SDS-PAGE of Chemicon's purified Human Vitronectin (Catalog Number CC080).
Vitronectin is a cell adhesion and spreading protein found in the ECM. Vitronectin interacts with c
CC080
100 µg  
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      Overview

      Key Spec Table

      Key Applications
      PC, CULT
      Description
      Catalogue Number CC080
      Brand Family Chemicon®
      Synonyms S-protein
      Trade Name
      • Chemicon
      Description Vitronectin, Human Purified Protein
      Overview PROPERTIES:

      Binds to heparin. Active in cell attachment of MG63 cells.
      Background Information Vitronectin (S-protein) is a multi-functional plasma and extracellular matrix protein with defined activity in cell adhesion, thrombosis, complement activation, fibrinolysis, inflammation, and platelet adhesion. Vitronectin binds to other macromolecules and participates in regulatory circuits to control these processes. Some target macromolecules that interact with vitronectin are heparin, PAI-1, proteases such as thrombin and urokinase-type plasminogen activator (uPA), serine protease inhibitor-protease complexes, the uPA receptor, and a sub-class of integrin receptors on the surface of cells. Vitronectin is also applied as a thin coating on tissue-culture surfaces, which is useful to promote cell attachment, spreading, proliferation, and differentiation of many normal and neoplastic cells, and to study cell migration.
      References
      Product Information
      Presentation Liquid in 50 mM sodium phosphate, 100 mM NaCl, pH 7.4, containing no preservatives.
      Applications
      Key Applications
      • Positive Control
      • Cell Culture
      Application Notes Suitable for use in ELISA, immunization, cell attachment assays and electrophoresis.
      Biological Information
      Concentration 1 mg/mL
      Purity >/= 90% by SDS-PAGE. Two bands in SDS-PAGE at 65 kDa and 75 kDa.
      Source Human plasma; tested negative for antibodies to HBsAg and HIV.
      Species Human
      Specific Activity Active in cell attachment assays at 1 μg/mL.
      Entrez Gene Number
      Entrez Gene Summary The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.
      Gene Symbol
      • VTN
      • V75
      • VN
      • VNT
      • epibolin
      • S-protein
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: P04004 # Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
      SIZE: 478 amino acids; 54306 Da
      SUBUNIT: Exists in two forms: a single chain 75 kDa form (V75) and a clipped form composed of two chains (65 kDa and 10 kDa) (V65+V10) which are held together by a disulfide bond. Interacts with SERPINE1/PAI1 and insulin.
      SUBCELLULAR LOCATION: Secreted, extracellular space.
      TISSUE SPECIFICITY: Plasma.
      DOMAIN: SwissProt: P04004 The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
      PTM: Sulfated on 2 tyrosine residues. & N- and O-glycosylated (By similarity). & Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading. & It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.SIMILARITY: Contains 4 hemopexin-like domains. & Contains 1 SMB (somatomedin-B) domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable 12 months at -70°C. Avoid repeated freeze/thaw cycles.

      During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.
      Packaging Information
      Material Size 100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      HUMAN PLASMA VITRONECTIN PURIFIED PROTEIN - 2383538 2383538
      HUMAN PLASMA VITRONECTIN PURIFIED PROTEIN - 2455645 2455645
      HUMAN PLASMA VITRONECTIN PURIFIED PROTEIN - 2022036 2022036
      HUMAN PLASMA VITRONECTIN PURIFIED PROTEIN - 2039780 2039780
      HUMAN PLASMA VITRONECTIN PURIFIED PROTEIN - 2055449 2055449
      HUMAN PLASMA VITRONECTIN PURIFIED PROTEIN - 2102009 2102009
      HUMAN PLASMA VITRONECTIN PURIFIED PROTEIN - 2167164 2167164
      HUMAN PLASMA VITRONECTIN PURIFIED PROTEIN - 2297236 2297236
      HUMAN PLASMA VITRONECTIN PURIFIED PROTEIN - 2324548 2324548

      References

      Reference overviewPub Med ID
      Src, PKCalpha, and PKCdelta are required for alphavbeta3 integrin-mediated metastatic melanoma invasion.
      Putnam, Andrew J, et al.
      Cell Commun. Signal, 7: 10 (2009) 2009

      Show Abstract
      19400942

      Brochure

      Title
      Human Stem Cell Systems

      Data Sheet

      Title
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation
      Vitronectin, Human Purified Protein - Data Sheet