CBL234F | Anti-Cytokeratin Pan Antibody, clone C11, FITC conjugated

CBL234F
100  assays
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Fg, B, H, RIH(P)MFITCMonoclonal Antibody
      Description
      Catalogue Number CBL234F
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      Description Anti-Cytokeratin Pan Antibody, clone C11, FITC conjugated
      References
      Product Information
      Format FITC
      Specificity The antibody reacts specifically with a wide range of normal, reactive and neoplastic epithelial tissues.

      KNOWN SPECIES CROSS REACTIVITY: Positive with bovine, rat and frog
      Presentation The conjugate is supplied as a 100 test vial in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. For flow cytometry we recommend using 10μL of conjugate per test.
      Applications
      Application Anti-Cytokeratin Pan Antibody, clone C11, FITC conjugated is an antibody against Cytokeratin Pan for use in IH(P).
      Key Applications
      • Immunohistochemistry (Paraffin)
      Application Notes Western blotting

      Pan epithelial reactivity on frozen sections

      Methanol/acetone fixed cultured epithelial cells

      Suitable for staining formalin fixed paraffin wax sections following proteolytic enzyme digestion

      Optimal working dilutions must be determined by the end user.
      Biological Information
      Immunogen species Epidermal carcinoma cell line A431
      Clone C-11
      Host Mouse
      Specificity The antibody reacts specifically with a wide range of normal, reactive and neoplastic epithelial tissues.

      KNOWN SPECIES CROSS REACTIVITY: Positive with bovine, rat and frog
      Isotype IgG1
      Species Reactivity Frog Bovine Human Rat
      Antibody Type Monoclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in differentiated layers of the mucosal and esophageal epithelia with family member KRT13. Mutations in these genes have been associated with White Sponge Nevus, characterized by oral, esophageal, and anal leukoplakia. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
      Gene Symbol
      • FLJ31692
      • KRT4
      • Cytokeratin-4
      • CK-4
      • CK4
      • CYK4
      • Keratin-4
      • K4
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: P05787 # Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
      SIZE: 483 amino acids; 53704 Da
      SUBUNIT: Heterotetramer of two type I and two type II keratins. keratin-8 associates with keratin-18. Associates with KRT20. Interacts with HCV core protein and PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP.
      SUBCELLULAR LOCATION: Cytoplasm.
      TISSUE SPECIFICITY: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
      PTM: Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
      DISEASE:SwissProt: P05787 # Defects in KRT8 are a cause of cryptogenic cirrhosis [MIM:215600].
      SIMILARITY: SwissProt: P05787 ## Belongs to the intermediate filament family.
      MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Store at +4°C protected from light. DO NOT FREEZE. For long term use and storage aliquot conjugate into small volumes and store at +4°C.
      Packaging Information
      Qty/Pck 100 assays
      Material Size 100
      Material Package assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-PAN CYTOKERATIN 2464602
      MOUSE ANTI-PAN CYTOKERATIN FITC CONJUGATED MONOCLONAL ANTIBODY - 2426988 2426988
      MOUSE ANTI-PAN CYTOKERATIN FITC CONJUGATED MONOCLONAL ANTIBODY - 2466295 2466295
      MOUSE ANTI-PAN CYTOKERATIN FITC CONJUGATED MONOCLONAL ANTIBODY - 2274282 2274282
      MOUSE ANTI-PAN CYTOKERATIN FITC CONJUGATED MONOCLONAL ANTIBODY - 2352391 2352391
      Millipore - COA - MOUSE ANTI-PAN CYTOKERATIN FITC CONJUGATED MONOCLONAL ANTIBODY - 2118954 2118954
      Millipore - COA - MOUSE ANTI-PAN CYTOKERATIN FITC CONJUGATED MONOCLONAL ANTIBODY - 1994903 1994903
      Millipore - COA - MOUSE ANTI-PAN CYTOKERATIN FITC CONJUGATED MONOCLONAL ANTIBODY - 2002492 2002492
      Millipore - COA - MOUSE ANTI-PAN CYTOKERATIN FITC CONJUGATED MONOCLONAL ANTIBODY - 2008999 2008999
      Millipore - COA - MOUSE ANTI-PAN CYTOKERATIN FITC CONJUGATED MONOCLONAL ANTIBODY - 2064292 2064292

      References

      Reference overviewPub Med ID
      A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology
      Bartek, J. et al.
      J. Pathol., 164:215-224 (1991) 1991

      1716305
      Effects of tissue fixation conditions and protease pretreatment on immunohistochemical performance of a large series of new anti-keratin monoclonal antibodies: value in oncopathology.
      Bártková, J, et al.
      Neoplasma, 38: 439-46 (1991) 1991

      1717857
      Use of keratin antibodies in tumor diagnosis.
      Lane, E B and Alexander, C M
      Semin. Cancer Biol., 1: 165-79 (1990) 1990

      Show Abstract
      1715788
      Patterns of expression of keratin 19 as detected with monoclonal antibodies in human breast tissues and tumours.
      Bartek, J, et al.
      Int. J. Cancer, 36: 299-306 (1985) 1985

      2411673

      Data Sheet

      Title
      Anti-Cytokeratin Pan, Clone C-11, FITC Conjugated - Data Sheet