APT800 | Apoptosis Inducer Set

APT800
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      Overview

      Key Spec Table

      Species ReactivityKey Applications
      MaACT
      Description
      Catalogue Number APT800
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      Description Apoptosis Inducer Set
      Background Information Actinomycin D: Classical anti-neoplastic drug. Cytotoxic inducer of apoptosis against tumor cells. A DNA dependent inhibitor of RNA synthesis, actinomycin promotes induction of apoptosis by some specific stimuli, for example, TRAIL and Fas (CD95). Actinomycin D can also alleviate or block the apoptotic process and decrease the cytotoxicity induced by several stimuli such as the dihydrofolate reductase inhibitor aminopterin and the prostaglandin derivative 15-deoxy-D12,14-prostaglandin J2, thus it can have both pro and ant-apoptotic activities in some systems.



      Camptothecin: Powerful anti-tumor agent. Camptoethecin is an inhibitor of DNA-topoisomerase I. The drug activates the p53 resulting in upregulated expression of TRAIL-R2 (DR5) and Bak to overcome TRAIL resistance in Bax-deficient human colon carcinoma cancer cells. In addition, Camptoethecin induced apoptosis can be increased by the presence of cytokines such as TNF via its subsequent inhibition of NFkappa B {Valente P et al Eur J Cancer (2003) 39:1468-77}.



      Cycloheximide: A potent protein translation inhibitor which results in the inhibition of protein synthesis and subsequent arrest of cell growth and induction of cell death. Cycloheximide also facilitates apoptosis induction via FasL, however, cycloheximide can also prevent apoptosis in some systems, such as thalassemic serum induction of HUVEC cells {Banjerdpong, R. (1998) Ph.D Thesis Mahidol Univ. Bangkok, Thailand}.



      Dexamethasone: Classic inducer of apoptosis in thymocytes; a glucocorticoid anti-inflammatory agent. Regulates T cell survival, growth and differientiation; modulator of nitric oxide synthase. Induces apoptosis in thymocytes as well as other immune cells like microglia. However, like other apoptotic inducers, dexamethasone can suppress apoptosis in certain systems through the activation of anti-apoptotic proteins such as bcl-X {Chang, et al (1997) FEBS Letter 415:11-15}.



      Etoposide: Potent topoisomerase II inhibitor. An anti-tumor agent that enhances dsDNA & ssDNA cleavage and inhibits DNA religation. Inhibits the cell cycle in the S-phase and induces apoptosis in normal and tumor cell lines, but can be modulated by intracellular levels of cAMP and other factors {Bermejo, L et al (1998) J. Cell Sci 111:637-644;Solovyyan, V et al. (1998) Brain Res Mol Brain Res 62:43-55}
      References
      Product Information
      Components
      • · Etoposide (10 mM): 100 μL
      • · Actinomycin D (10 mM): 50 μL
      • · Camptothecin (2 mM): 1 mL
      • · Cycloheximide (100 mM): 1 mL
      • · Dexamethasone (10 mM): 1 mL
      Presentation Liquid in DMSO containing no preservatives.
      Applications
      Application Apoptosis Inducer Set contains Actinomycin D, Camptothecin, Cycloheximide, Dexamethasone & Etoposide. These compounds can be used for inducing apoptotic activity in many cell culture systems.
      Key Applications
      • Activity Assay
      Application Notes It is recommended to use 1000X dilutions of the supplied stocks as a starting point for inducing apoptotic activity in many cell culture systems (e.g., Add 1 μL to 1 mL culture medium), however, the optimal doses may vary for different cell and culture conditions. For small volumes diluting the stocks 1:10 into fresh DMSO, then diluting the material further 1:100 into the culture media or PBS can facilitate the addition of the inhibitor to small volumes of less than 1 mL.
      Biological Information
      Species Reactivity Mammals
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Store kit materials at -20°C for up to their expiration date. Avoid repeated freeze/thaw cycles. Protect product tubes from light.



      WARNING: Kit contains highly toxic agents; professional users only; wear gloves and other protective equipment and good laboratory practices when manipulating the concentrated and diluted solutions.
      Packaging Information
      Material Size 1 kit
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Apoptosis Inducer Set - 2151077 2151077
      Apoptosis Inducer Set - 1984851 1984851
      Apoptosis Inducer Set - 2074895 2074895
      Apoptosis Inducer Set - 2199751 2199751
      Apoptosis Inducer Set - 2251944 2251944

      References

      Reference overviewPub Med ID
      Cell death and tissue remodeling in planarian regeneration.
      Jason Pellettieri,Patrick Fitzgerald,Shigeki Watanabe,Joel Mancuso,Douglas R Green,Alejandro Sánchez Alvarado
      Developmental biology 338 2010

      Show Abstract Full Text Article
      19766622