ABC458 | Anti-Thymidylate Kinase/TMPK Antibody

Western Blotting Analysis: Representative lot data. Jurkat cell lysate was probed with Cat. No. ABC458, Anti-Thymidylate Kinase/TMPK (2 µg/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Thymidylate Kinase/TMPK (~24 kDa).
Western Blotting Analysis: Representative lot data. Jurkat cell lysate was probed with Cat. No. ABC4
NewABC458
100  μg
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number ABC458
      Synonyms dTMP kinase Thymidylate kinase
      Description Anti-Thymidylate Kinase/TMPK Antibody
      Background Information TMPK, also known as dTMP kinase, and encoded by the gene DTYMK/CDC8/TMPK/TYMK, is a key enzyme for pyrimidine synthesis that catalyzes the phosphorylation of thymidine 5'-monophosphate (dTMP) to form thymidine 5'-diphosphate (dTDP). TMPK has important functions in cell proliferation and DNA replication, and the synthesis of dTDP is unique because it requires TMPK. All other dNDPs are directly produced by ribonucleotide reductase (RNR). Thus, TMPK is well recognized as a potential drug target for many diseases and disease agents including viruses. TMPK is currently a target of anti-HIV nucleoside therapy, and TMPK is also a validated target for antibiotic development against resistance strains of bacteria and parasites, and research demonstrates that it may also be a good target for the anticancer agent doxorubicin cancer cells. Expressed by virtually all cells it is localized to cell cytoplasm with only traces found in Golgi, mitochondria and nuclei.
      References
      Product Information
      Format Purified
      Presentation Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      Application Anti-Thymidylate Kinase/TMPK Antibody is a rabbit polyclonal antibody, validated for use in western blotting & IP.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application Notes Western Blotting Analysis: A representative lot from an independent laboratory detected Thymidylate Kinase/TMPK in MDA-MB231, MCF-7, H184B5F5/M10 cell lysates (Hu, C. M., et al. (2012). Cancer Cell. 22(1):36-50.).

      Immunoprecipitation Analysis: A representative lot from an independent laboratory immunprecipitated Thymidylate Kinase/TMPK from MCF-7, H184B4F5/M10, and MCF-10A cell lysates (Ke, P. Y., et al. (2005). Genes Dev. 19(16):1920-1933.).

      Western Blotting Analysis: A representative lot from an independent laboratory detected Thymidylate Kinase/TMPK in MCF-7, H184B4F5/M10, and MCF-10A cell lysates (Ke, P. Y., et al. (2005). Genes Dev. 19(16):1920-1933.).
      Biological Information
      Immunogen species GST-tagged recombinant protein corresponding to human Thymidylate Kinase/TMPK.
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Rabbit
      Species Reactivity Human
      Antibody Type Polyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TYMK
      • DTYMK
      • CDC8
      • TMPK
      Purification Method Protein A purified
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Evaluated by Western Blotting in Jurkat cell lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected Thymidylate Kinase/TMPK in 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Qty/Pck 100 μg
      Material Size 100
      Material Package μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-Thymidylate Kinase/TMPK - Q2341232 Q2341232

      References

      Reference overviewPub Med ID
      Control of dTTP pool size by anaphase promoting complex/cyclosome is essential for the maintenance of genetic stability.
      Ke, Po-Yuan, et al.
      Genes Dev., 19: 1920-33 (2005) 2005

      Show Abstract
      16103219