AB5922 | Anti-Nestin Antibody

Immunocytochemistry: Representative image from a previous lot.
Rabbit anti-Nestin (Catalog Number AB5922) Staining of U251 cells. Shown are cells stained with anti-Nestin (green) and bisbenzimide (blue). Photo courtesy of Dr. Conrad Messam, Dr. Jean Hou, and Dr. Eugene Major, NINDS, NIH, Bethesda, MD. Reprinted from Experimental Neurology (2000) 161:585-596.
Immunocytochemistry: Representative image from a previous lot.
Rabbit anti-Nestin (Catalog Numb
AB5922
50  µL
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, IHC, IH(P)RbSerumPolyclonal Antibody
      Description
      Catalogue Number AB5922
      Brand Family Chemicon®
      Synonyms Nestin
      Trade Name
      • Chemicon
      Description Anti-Nestin Antibody
      Background Information Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.
      References
      Product Information
      Format Serum
      Specificity Human Nestin. The antibody shows no reactivity to vimentin or GFAP.
      Control
      • in U251 (Human Glioma line) nestin migrates at ~260 kDa.
      • Positive controls are U251 or U373 human glioma cell lines. Detects a ~200-220 kDa protein, note slightly difference sizes for nestin are quoted in various paper and from various cell lines
      • in WI-38 (human lung line) reduced nestin migrates at ~220 kDa
      Presentation Rabbit polyclonal Serum in buffer containing Liquid.
      Applications
      Application Anti-Nestin Antibody is an antibody against Nestin for use in IC, IH, IH(P) & WB.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      Application Notes Western Blot:
      1:1,000-1:5,000 dilution of a previous lot was used on cultured cells or human brain tissue. It is suggested that the membrane be blocked with PBS containing 5% nonfat milk and 0.05% Tween. The antibody may also be diluted in the same blocking buffer.

      Immunocytochemistry:
      1:200 dilution of a previous lot was used on cells fixed in 4% paraformaldehyde and permeabilized with 0.02% Triton.

      Immunohistochemistry(paraffin):
      1:200 dilution of a previous lot was used on formalin fixed, paraffin embedded tissue sections.
      Biological Information
      Immunogen species Fusion protein.
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Rabbit
      Specificity Human Nestin. The antibody shows no reactivity to vimentin or GFAP.
      Isotype IgG
      Species Reactivity Human
      Species Reactivity Note Human. No reactivity to rat or mouse nestin. Other species have not yet been tested
      Antibody Type Polyclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]
      Gene Symbol
      • nestin
      • FLJ21841
      • Nbla00170
      • OTTHUMP00000032198
      Non-Reactive Species
      • Rat
      • Mouse
      Purification Method Unpurified
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: P48681 # May play a role in the trafficking and distribution of IF proteins and potentially other cellular factors to daughter cells during progenitor cell division (By similarity).
      SIZE: 1621 amino acids; 177439 Da
      SUBUNIT: Interacts with FHOD3 (By similarity).
      TISSUE SPECIFICITY: CNS stem cells.
      DEVELOPMENTAL STAGE: Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
      SIMILARITY: SwissProt: P48681 ## Belongs to the intermediate filament family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Qty/Pck 50 µL
      Material Size 50
      Material Package µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Millipore - COA - Anti-Nestin - LV1635955 LV1635955
      Millipore - COA - Anti-Nestin - LV1751778 LV1751778
      Millipore - COA - Anti-Nestin - NG1853940 NG1853940
      Millipore - COA - RABBIT ANTI-NESTIN POLYCLONAL ANTIBODY - LV1519217 LV1519217

      References | 31 Available | See All References

      Reference overviewSpeciesPub Med ID
      Different expression patterns of Phactr family members in normal and injured mouse brain.
      J Y Kim,S Y Choi,Y Moon,H J Kim,J H Chin,H Kim,W Sun
      Neuroscience 221 2012

      Show Abstract
      22766235
      Glioblastoma resistance to anti-VEGF therapy is associated with myeloid cell infiltration, stem cell accumulation, and a mesenchymal phenotype.
      Yuji Piao,Ji Liang,Lindsay Holmes,Amado J Zurita,Verlene Henry,John V Heymach,John F de Groot
      Neuro-oncology 14 2012

      Show Abstract
      22965162
      Girdin maintains the stemness of glioblastoma stem cells.
      A Natsume,T Kato,S Kinjo,A Enomoto,H Toda,S Shimato,F Ohka,K Motomura,Y Kondo,T Miyata,M Takahashi,T Wakabayashi
      Oncogene 31 2012

      Show Abstract
      22020337
      Osteoblastic expansion induced by parathyroid hormone receptor signaling in murine osteocytes is not sufficient to increase hematopoietic stem cells.
      Laura M Calvi,Olga Bromberg,Yumie Rhee,Jonathan M Weber,Julianne N P Smith,Miles J Basil,Benjamin J Frisch,Teresita Bellido
      Blood 119 2012

      Show Abstract
      22262765
      Spatio-temporal dynamics, differentiation and viability of human neural stem cells after implantation into neonatal rat brain.
      Kallur T, Farr TD, Böhm-Sturm P, Kokaia Z, Hoehn M
      The European journal of neuroscience 34 382-93. doi 2011

      Show Abstract
      21707793
      Complement Receptor 2 Is Expressed in Neural Progenitor Cells and Regulates Adult Hippocampal Neurogenesis.
      Moriyama M, Fukuhara T, Britschgi M, He Y, Narasimhan R, Villeda S, Molina H, Huber BT, Holers M, Wyss-Coray T
      J Neurosci 31 3981-3989. 2011

      Show Abstract Full Text Article
      21411641
      Secreted factors from brain endothelial cells maintain glioblastoma stem-like cell expansion through the mTOR pathway.
      Galan-Moya, Eva Maria, et al.
      EMBO Rep., 12: 470-6 (2011) 2011

      Show Abstract
      21460795
      Ginsenoside Rd attenuates mitochondrial dysfunction and sequential apoptosis after transient focal ischemia.
      Ye R, Zhang X, Kong X, Han J, Yang Q, Zhang Y, Chen Y, Li P, Liu J, Shi M, Xiong L, Zhao G
      Neuroscience 2011

      Show Abstract
      21219973
      Doublecortin-positive cells in the adult primate cerebral cortex and possible role in brain plasticity and development.
      Jocelyne Bloch,Mélanie Kaeser,Yalda Sadeghi,Eric M Rouiller,D Eugene Redmond,Jean-François Brunet
      The Journal of comparative neurology 519 2011

      Show Abstract
      21246554
      Expression of embryonic stem cell markers SOX2 and nestin in dermatofibrosarcoma protuberans and dermatofibroma.
      Klaus Sellheyer,Paula Nelson,Rajiv M Patel
      Journal of cutaneous pathology 38 2011

      Show Abstract
      21251041

      Brochure

      Title
      Western Blotting Tools

      Data Sheet

      Title
      RABBIT ANTI-NESTIN POLYCLONAL ANTIBODY