AB5535 | Anti-Sox9 Antibody

Western Blot Analysis:
HepG2 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-SOX-9 (1:500 dilution of this lot).
Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chem
Western Blot Analysis:
HepG2 lysate was resolved by electrophoresis, transferred to PVDF membra
AB5535
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, MIHC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number AB5535
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      Description Anti-Sox9 Antibody
      Background Information Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at a minimum of 40 different loci that rapidly diverged in various animal lineages. At present 30 Sox genes have been identified, and members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.
      References
      Product Information
      Format Affinity Purified
      Specificity Recognizes Sox9.
      Control
      • Embryonic tissue, Adult chondrocytes.
      Presentation Purified rabbit polyclonal in buffer containing 0.1M Tris -Glycine, 0.15M NaCl, 0.05% sodium azide, pH7.4.
      Applications
      Application Anti-Sox9 Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Sox9 & has been validated in IHC & WB.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application Notes Western Blot:
      1:500 dilution of a previous lot ~65kDa band

      EMSA supershift

      Optimal working dilutions must be determined by the end user.
      Biological Information
      Immunogen species Synthetic peptide from human Sox9.
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Rabbit
      Specificity Recognizes Sox9.
      Species Reactivity Human Rat Mouse
      Species Reactivity Note Human, rat and fetal mouse. Other species have not yet been tested.
      Antibody Type Polyclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.
      Gene Symbol
      • SOX9
      • SRA1
      • CMD1
      • CMPD1
      Purification Method Immunoaffinity purified
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: P48436 # Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
      SIZE: 509 amino acids; 56137 Da
      SUBCELLULAR LOCATION: Nucleus (Potential).
      DISEASE: SwissProt: P48436 # Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male- to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
      SIMILARITY: SwissProt: P48436 ## Contains 1 HMG box DNA-binding domain
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Routinely evaluated by Western Blot on L6 cell lysates.

      Western Blot Analysis: 1:2000 dilution of this lot detected SOX-9 on 10 μg of L6 cell lysate
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
      Packaging Information
      Material Size 100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Languages
      Deutschland

      Certificates of Analysis

      TitleLot Number
      Anti-Sox9 - 2383973 2383973
      Anti-Sox9 - 2424751 2424751
      Anti-Sox9 - 2462860 2462860
      Anti-Sox9 - 1969702 1969702
      Anti-Sox9 - 1997395 1997395
      Anti-Sox9 - 2031330 2031330
      Anti-Sox9 - 2065999 2065999
      Anti-Sox9 - 2167153 2167153
      Anti-Sox9 - 2262679 2262679
      Anti-Sox9 - 2361818 2361818
      Anti-Sox9 - 2494029 2494029
      Anti-Sox9 - LV1478872 LV1478872
      Anti-Sox9 - LV1580835 LV1580835
      Anti-Sox9 - LV1679322 LV1679322
      Anti-Sox9 - LV1738269 LV1738269
      Anti-Sox9 - LV1762669 LV1762669
      Anti-Sox9 - NG1839530 NG1839530
      Anti-Sox9 - NG1884650 NG1884650
      Anti-Sox9 - NG1901550 NG1901550
      Anti-Sox9 - NG1922963 NG1922963
      RABBIT ANTI-POTASSIUM CHANNEL MAXI K (BKCa) - 2494610 2494610

      References | 52 Available | See All References

      Reference overviewPub Med ID
      GATA4 and GATA6 control mouse pancreas organogenesis.
      Manuel Carrasco,Irene Delgado,Bernat Soria,Francisco Mart,Anabel Rojas
      The Journal of clinical investigation 122 2012

      Show Abstract
      23006330
      Testicular development in mice lacking receptors for follicle stimulating hormone and androgen.
      Peter J O'Shaughnessy,Ana Monteiro,Margaret Abel
      PloS one 7 2012

      Show Abstract
      22514715
      Regulation of human lung alveolar multipotent cells by a novel p38α MAPK/miR-17-92 axis.
      Feride Oeztuerk-Winder,Anna Guinot,Anna Ochalek,Juan-Jose Ventura
      The EMBO journal 31 2012

      Show Abstract
      22828869
      Sox9 directs hypertrophic maturation and blocks osteoblast differentiation of growth plate chondrocytes.
      Peter Dy,Weihuan Wang,Pallavi Bhattaram,Qiuqing Wang,Lai Wang,R Tracy Ballock,Véronique Lefebvre
      Developmental cell 22 2012

      Show Abstract
      22421045
      BAC-Dkk3-EGFP transgenic mouse: an in vivo analytical tool for Dkk3 expression.
      Yuki Muranishi,Takahisa Furukawa
      Journal of biomedicine & biotechnology 2012 2012

      Show Abstract
      22910798
      Isolation, differentiation, and characterisation of skeletal stem cells from human bone marrow in vitro and in vivo.
      Rahul S Tare,Peter D Mitchell,Janos Kanczler,Richard O C Oreffo
      Methods in molecular biology (Clifton, N.J.) 816 2012

      Show Abstract
      22130924
      Oncogenicity of the developmental transcription factor Sox9.
      Ander Matheu,Manuel Collado,Clare Wise,Lorea Manterola,Lina Cekaite,Angela J Tye,Marta Canamero,Luis Bujanda,Andreas Schedl,Kathryn S E Cheah,Rolf I Skotheim,Ragnhild A Lothe,Adolfo López de Munain,James Briscoe,Manuel Serrano,Robin Lovell-Badge,Adolfo L
      Cancer research 72 2012

      Show Abstract
      22246670
      Slug and Sox9 cooperatively determine the mammary stem cell state.
      Guo, Wenjun, et al.
      Cell, 148: 1015-28 (2012) 2012

      Show Abstract
      22385965
      The maturation of photoreceptors in the avian retina is stimulated by thyroid hormone.
      Fischer AJ, Bongini R, Bastaki N, Sherwood P
      Neuroscience 178 250-60. Epub 2011 Jan 19. 2011

      Show Abstract Full Text Article
      21256198
      An EGFR-ERK-SOX9 signaling cascade links urothelial development and regeneration to cancer.
      Ling S, Chang X, Schultz L, Lee TK, Chaux A, Marchionni L, Netto GJ, Sidransky D, Berman DM
      Cancer research 71 3812-21. Epub 2011 Apr 21. 2011

      Show Abstract
      21512138

      Brochure

      Title
      SNAP i.d. 2.0 System Brochure

      Data Sheet

      Title
      Anti-Sox9 - Data Sheet