AB5535 | Anti-Sox9 Antibody

Immunohistochemistry Analysis:
Representative lot data.
Formalin Fixed Paraffin Embedded (FFPE) murine embryonic developing bone (Panels A&B) and adult murine cartilage (Panel C) tissue sections were processed using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:500 dilution of Cat. No. AB5535, Anti-Sox9. Reactivity was detected using an Anti-Rabbit secondary antibody and HRP-DAB. Positive nuclear staining was observed in the tissue sections.
Immunohistochemistry Analysis:
Representative lot data.
Formalin Fixed Paraffin Embedded (
100 µg  
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      Replacement Information

      Key Spec Table

      Species Reactivity Key Applications Host Format Antibody Type
      H, R, MIHC, WBRbAffinity PurifiedPolyclonal Antibody
      Catalogue Number AB5535
      Replaces AB5809
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      Description Anti-Sox9 Antibody
      Background Information Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at a minimum of 40 different loci that rapidly diverged in various animal lineages. At present 30 Sox genes have been identified, and members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.
      Product Information
      Format Affinity Purified
      • Embryonic tissue, Adult chondrocytes.
      Presentation Purified rabbit polyclonal in buffer containing 0.1M Tris -Glycine, 0.15M NaCl, 0.05% sodium azide, pH7.4.
      Application Anti-Sox9 Antibody is a well characterized affinity purified Rabbit Polyclonal Antibody that reliably detects Transcription Factor Sox-9. This highly published antibody has been validated in IHC & WB.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application Notes Western Blot:
      1:500 dilution of a previous lot ~65kDa band

      EMSA supershift

      Optimal working dilutions must be determined by the end user.
      Biological Information
      Immunogen Synthetic peptide from human Sox9.
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Rabbit
      Specificity Recognizes Sox9.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Species Reactivity Note Human, rat and fetal mouse. Other species have not yet been tested.
      Antibody Type Polyclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.
      Gene Symbol
      • SOX9
      • SRA1
      • CMD1
      • CMPD1
      Purification Method Immunoaffinity purified
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: P48436 # Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
      SIZE: 509 amino acids; 56137 Da
      SUBCELLULAR LOCATION: Nucleus (Potential).
      DISEASE: SwissProt: P48436 # Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male- to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
      SIMILARITY: SwissProt: P48436 ## Contains 1 HMG box DNA-binding domain
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Routinely evaluated by Western Blot on L6 cell lysates.

      Western Blot Analysis: 1:2000 dilution of this lot detected SOX-9 on 10 μg of L6 cell lysate
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
      Packaging Information
      Material Size 100 µg
      Transport Information
      Supplemental Information




      Certificates of Analysis

      TitleLot Number
      Anti-Sox9 - 23839732383973
      Anti-Sox9 - 24247512424751
      Anti-Sox9 - 24628602462860
      Anti-Sox9 - 19697021969702
      Anti-Sox9 - 19973951997395
      Anti-Sox9 - 20313302031330
      Anti-Sox9 - 20659992065999
      Anti-Sox9 - 21671532167153
      Anti-Sox9 - 22626792262679
      Anti-Sox9 - 23618182361818
      Anti-Sox9 - 24940292494029
      Anti-Sox9 - LV1478872LV1478872
      Anti-Sox9 - LV1580835LV1580835
      Anti-Sox9 - LV1679322LV1679322
      Anti-Sox9 - LV1738269LV1738269
      Anti-Sox9 - LV1762669LV1762669
      Anti-Sox9 - NG1839530NG1839530
      Anti-Sox9 - NG1884650NG1884650
      Anti-Sox9 - NG1901550NG1901550
      Anti-Sox9 - NG1922963NG1922963
      Anti-Sox9 -25103212510321
      Anti-Sox9 -25522812552281
      Anti-Sox9 -25857852585785
      Anti-Sox9 -25938672593867
      Anti-Sox9 -26375052637505

      References | 54 Available | See All References

      Reference overviewPub Med ID
      Mutant IDH inhibits HNF-4α to block hepatocyte differentiation and promote biliary cancer.
      Saha, SK; Parachoniak, CA; Ghanta, KS; Fitamant, J; Ross, KN; Najem, MS; Gurumurthy, S; Akbay, EA; Sia, D; Cornella, H; Miltiadous, O; Walesky, C; Deshpande, V; Zhu, AX; Hezel, AF; Yen, KE; Straley, KS; Travins, J; Popovici-Muller, J; Gliser, C; Ferrone, CR; Apte, U; Llovet, JM; Wong, KK; Ramaswamy, S; Bardeesy, N
      Nature 513 110-4 2014

      Show Abstract
      GATA4 and GATA6 control mouse pancreas organogenesis.
      Manuel Carrasco,Irene Delgado,Bernat Soria,Francisco Mart,Anabel Rojas
      The Journal of clinical investigation 122 2012

      Show Abstract
      Testicular development in mice lacking receptors for follicle stimulating hormone and androgen.
      Peter J O'Shaughnessy,Ana Monteiro,Margaret Abel
      PloS one 7 2012

      Show Abstract
      Regulation of human lung alveolar multipotent cells by a novel p38α MAPK/miR-17-92 axis.
      Feride Oeztuerk-Winder,Anna Guinot,Anna Ochalek,Juan-Jose Ventura
      The EMBO journal 31 2012

      Show Abstract
      Sox9 directs hypertrophic maturation and blocks osteoblast differentiation of growth plate chondrocytes.
      Peter Dy,Weihuan Wang,Pallavi Bhattaram,Qiuqing Wang,Lai Wang,R Tracy Ballock,Véronique Lefebvre
      Developmental cell 22 2012

      Show Abstract
      BAC-Dkk3-EGFP transgenic mouse: an in vivo analytical tool for Dkk3 expression.
      Yuki Muranishi,Takahisa Furukawa
      Journal of biomedicine & biotechnology 2012 2012

      Show Abstract
      Isolation, differentiation, and characterisation of skeletal stem cells from human bone marrow in vitro and in vivo.
      Rahul S Tare,Peter D Mitchell,Janos Kanczler,Richard O C Oreffo
      Methods in molecular biology (Clifton, N.J.) 816 2012

      Show Abstract
      Oncogenicity of the developmental transcription factor Sox9.
      Ander Matheu,Manuel Collado,Clare Wise,Lorea Manterola,Lina Cekaite,Angela J Tye,Marta Canamero,Luis Bujanda,Andreas Schedl,Kathryn S E Cheah,Rolf I Skotheim,Ragnhild A Lothe,Adolfo López de Munain,James Briscoe,Manuel Serrano,Robin Lovell-Badge,Adolfo L
      Cancer research 72 2012

      Show Abstract
      Slug and Sox9 cooperatively determine the mammary stem cell state.
      Guo, Wenjun, et al.
      Cell, 148: 1015-28 (2012) 2012

      Show Abstract
      The maturation of photoreceptors in the avian retina is stimulated by thyroid hormone.
      Fischer AJ, Bongini R, Bastaki N, Sherwood P
      Neuroscience 178 250-60. Epub 2011 Jan 19. 2011

      Show Abstract Full Text Article


      SNAP i.d. 2.0 System Brochure

      Data Sheet

      Anti-Sox9 - Data Sheet