AB5204 | Anti-Sodium Channel Antibody, Voltage Gated, Brain Type I

AB5204
200 µL  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, RIHC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number AB5204
      Brand Family Chemicon®
      Synonyms Nav1.1; SCN1A
      Trade Name
      • Chemicon
      Description Anti-Sodium Channel Antibody, Voltage Gated, Brain Type I
      References
      Product Information
      Format Affinity Purified
      Specificity Recognizes type I alpha subunit of VGSC. Does not cross react with any other sodium channel antigens tested so far.

      SPECIES REACTIVITIES: It is expected that the antibody may also react with human due to sequence homology. Other species have not been tested.
      Control
      • CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
      Presentation Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
      Applications
      Application This Anti-Sodium Channel Antibody, Voltage Gated, Brain Type I is validated for use in IH, WB for the detection of Sodium Channel.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application Notes All procedures that are going to receive a full-length protein should be performed at 4C, and the following protease inhibitor mixture should be used: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each).

      Western blot: 1:200 using ECL on rat brain membranes.

      Immunohistochemistry on rat brain fixed frozen sections and mouse heart tissue.

      Dilutions should be made using a carrier protein such as BSA (1-3%)

      Optimal working dilutions must be determined by the end user.
      Biological Information
      Immunogen species Purified peptide from 465-481 of alpha subunit of rat type I voltage-gated sodium channel (VGSC) (Accession P04774).
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Rabbit
      Specificity Recognizes type I alpha subunit of VGSC. Does not cross react with any other sodium channel antigens tested so far.

      SPECIES REACTIVITIES: It is expected that the antibody may also react with human due to sequence homology. Other species have not been tested.
      Species Reactivity Mouse Rat
      Antibody Type Polyclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary The vertebrate sodium channel is a voltage-gated ion channel essential for the generation and propagation of action potentials, chiefly in nerve and muscle. Voltage-sensitive sodium channels are heteromeric complexes consisting of a large central pore-forming glycosylated alpha subunit and 2 smaller auxiliary beta subunits. Functional studies have indicated that the transmembrane alpha subunit of the brain sodium channels is sufficient for expression of functional sodium channels (Goldin et al., 1986 [PubMed 2429308]; Isom, 2002 [PubMed 11779698]).[supplied by OMIM]
      Gene Symbol
      • SCN1A
      • GEFSP2
      • SMEI
      • NAC1
      • FEB3
      • Nav1.1
      • HBSCI
      • SCN1
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: P35498 # Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.
      SIZE: 2009 amino acids; 228972 Da
      SUBUNIT: The sodium channel consists of a large polypeptide and 2- 3 smaller ones. This sequence represents a large polypeptide.
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      DOMAIN: SwissProt: P35498 The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
      DISEASE: SwissProt: P35498 # Defects in SCN1A are the cause of generalized epilepsy with febrile seizures plus type 2 (GEFS+2) [MIM:604233]. This autosomal dominant disorder is characterized by febrile seizures in children and afebrile seizures in adults. Penetrance is incomplete and a large intrafamilial variability of the phenotype is observed. & Defects in SCN1A are a cause of severe myoclonic epilepsy in infancy (SMEI) [MIM:607208]; also called Dravet syndrome. SMEI is a severe form of generalized epilepsy with febrile seizures. It is a rare disorder characterized by normal development before onset, seizures beginning in the first year of life in the form of generalized or unilateral febrile clonic seizures, secondary appearance of myoclonic seizures, and occasionally partial seizures. It is associated with ataxia, slowed psychomotor development, and mental decline. & Defects in SCN1A are the cause of familial hemiplegic migraine 3 (FHM3) [MIM:609634]. FHM3 is an autosomal dominant severe subtype of migraine with aura characterized by some degree of hemiparesis during the attacks. The episodes are associated with variable features of nausea, vomiting, photophobia and phonophobia. Age at onset ranges from 6 to 15 years. Some patients may manifest seizures during infancy. & Defects in SCN1A are the cause of familial febrile convulsions 3 (FEB3) [MIM:604403]; also known as familial febrile seizures 3. Febrile convulsions affect 5-12% of infants and children up to 6 years of age. There is epidemiological evidence that febrile seizures are associated with subsequent afebrile and unprovoked seizures in 2% to 7% of patients. Inheritance pattern is autosomal dominant.
      SIMILARITY: Belongs to the sodium channel family. & Contains 1 IQ domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size 200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-BRAIN TYPE I VOLTAGE GATED SODIUM CHANNEL AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2154059 2154059
      RABBIT ANTI-BRAIN TYPE I VOLTAGE GATED SODIUM CHANNEL AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2397061 2397061
      RABBIT ANTI-BRAIN TYPE I VOLTAGE GATED SODIUM CHANNEL AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2055427 2055427
      RABBIT ANTI-BRAIN TYPE I VOLTAGE GATED SODIUM CHANNEL AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2318080 2318080

      References

      Reference overviewPub Med ID
      Spontaneous epileptiform discharges in a mouse model of Alzheimer\'s disease are suppressed by antiepileptic drugs that block sodium channels.
      Ziyatdinova S, Gurevicius K, Kutchiashvili N, Bolkvadze T, Nissinen J, Tanila H, Pitkänen A
      Epilepsy research 94 75-85. 2011

      Show Abstract
      21300523
      Persistent Nav1.6 current at axon initial segments tunes spike timing of cerebellar granule cells.
      Nancy Osorio,Laurence Cathala,Miriam H Meisler,Marcel Crest,Jacopo Magistretti,Patrick Delmas
      The Journal of physiology 588 2010

      Show Abstract Full Text Article
      20173079
      A missense mutation of the gene encoding voltage-dependent sodium channel (Nav1.1) confers susceptibility to febrile seizures in rats.
      Mashimo T, Ohmori I, Ouchida M, Ohno Y, Tsurumi T, Miki T, Wakamori M, Ishihara S, Yoshida T, Takizawa A, Kato M, Hirabayashi M, Sasa M, Mori Y, Serikawa T
      J Neurosci 30 5744-53. 2010

      Show Abstract
      20410126
      Distinct subcellular localization of different sodium channel alpha and beta subunits in single ventricular myocytes from mouse heart
      Maier, Sebastian K G, et al
      Circulation, 109:1421-7 (2004) 2004

      15007009
      Neuronal death and perinatal lethality in voltage-gated sodium channel alpha(II)-deficient mice.
      Planells-Cases, R, et al.
      Biophys. J., 78: 2878-91 (2000) 2000

      Show Abstract
      10827969
      AnkyrinG is required for clustering of voltage-gated Na channels at axon initial segments and for normal action potential firing.
      Zhou, D, et al.
      J. Cell Biol., 143: 1295-304 (1998) 1998

      Show Abstract
      9832557

      Data Sheet

      Title
      RABBIT ANTI-BRAIN TYPE I VOLTAGE GATED SODIUM CHANNEL AFFINITY PURIFIED POLYCLONAL ANTIBODY

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies