AB1504 | Anti-Glutamate receptor 1 Antibody

Immunohistochemistry Analysis:
Representative lot data.
Paraffin-embedded rat brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:300 dilution of Cat. No. AB1504, Anti-Glutamate receptor 1. Reactivity was detected using the IHC-Select Detection Kit (Cat. No. DAB050). Staining pattern appears as cytoplasmic.
Immunohistochemistry Analysis:
Representative lot data.
Paraffin-embedded rat brain tissu
AB1504
200 µL  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Eq, R, M, HIP, WB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number AB1504
      Synonyms glutamate receptor, ionotropic, AMPA 1 AMPA 1 AMPA-selective glutamate receptor 1 Glutamate receptor ionotropic, AMPA 1
      Description Anti-Glutamate receptor 1 Antibody
      Background Information Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. GluR1 is believed to mediate calcium influx, and has a vital function in synaptic plasticity related to learning. Reduction in GluR1 has been caused by FMR1 gene mutation and contributes to fragile X syndrome. Changes in expression of GluR1 receptors on dopamine neurons have been correlated with sensitization of drugs of abuse.
      References
      Product Information
      Format Affinity Purified
      Specificity This antibody recognizes Glutamate receptor 1 at the cytoplasmic domain.
      Control
      • Mouse brain lysate
      Presentation Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      Application This Anti-Glutamate receptor 1 Antibody is validated for use in WB, IH(P), IP for the detection of Glutamate receptor 1.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application Notes Immunohistochemistry Analysis: 1:300 dilution of this antibody has been shown to detect Glutamate receptor 1 (GluR1) in rat brain tissue.
      Biological Information
      Immunogen species KLH-conjugated linear peptide corresponding to human Glutamate receptor 1 at the cytoplasmic domain.
      Epitope Cytoplasmic domain
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Rabbit
      Specificity This antibody recognizes Glutamate receptor 1 at the cytoplasmic domain.
      Species Reactivity Horse Rat Mouse Human
      Species Reactivity Note Demonstrated to react with human, mouse, and rat. Predicted to react with equine and platypus based on 100% sequence homology.
      Antibody Type Polyclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
      Gene Symbol
      • GluR-1
      • GRIA1
      • GluR-K1
      • GluR-A
      • GLUH1
      • GLUR1
      • GLURA
      • HBGR1
      Purification Method Affinity Purfied
      UniProt Number
      UniProt Summary FUNCTION: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.

      >SIZE: 906 amino acids; 101506 Da

      SUBUNIT: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with DLG1 via its C-terminus. Interacts with CACNG2 and RASGRF2 (By similarity).

      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Note=Interaction with CACNG2 promotes cell surface expression (By similarity).

      TISSUE SPECIFICITY: Widely expressed in brain.

      PTM: Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity).

      SIMILARITY: Belongs to the glutamate-gated ion channel (TC 1.A.10) family.

      MISCELLANEOUS: The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Evaluated by Western Blot in mouse brain lysate.

      Western Blot Analysis: 1:1000 dilution of this antibody detected Glutamate receptor 1 (GluR1) on 10 µg of mouse brain lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size 200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-GluR1 - LV1446043A LV1446043A
      Anti-GluR1 - LV1519215 LV1519215
      Anti-GluR1 - LV1563305 LV1563305
      Anti-GluR1 - LV1619032 LV1619032
      Anti-GluR1 - LV1620620 LV1620620
      Anti-GluR1 - LV1623825 LV1623825
      Anti-GluR1 - LV1726018 LV1726018
      Anti-Glutamate receptor 1 - 2121763 2121763
      Anti-Glutamate receptor 1 - 2328021 2328021
      Anti-Glutamate receptor 1 - 2428717 2428717
      Anti-Glutamate receptor 1 - 2459591 2459591
      Anti-Glutamate receptor 1 - 2019967 2019967
      Anti-Glutamate receptor 1 - 2035064 2035064
      Anti-Glutamate receptor 1 - 2193310 2193310
      Anti-Glutamate receptor 1 - 2207172 2207172
      Anti-Glutamate receptor 1 - 2294344 2294344
      Anti-Glutamate receptor 1 - 2510279 2510279
      Anti-Glutamate receptor 1 - LV1740049 LV1740049
      Anti-Glutamate receptor 1 - LV1759223 LV1759223
      Anti-Glutamate receptor 1 - LV1773598 LV1773598
      Anti-Glutamate receptor 1 - NG1841053 NG1841053
      Anti-Glutamate receptor 1 - NG1849977 NG1849977
      Anti-Glutamate receptor 1 - NG1902000 NG1902000
      Anti-Glutamate receptor 1 -2484242 2484242

      References | 48 Available | See All References

      Reference overviewApplicationSpeciesPub Med ID
      Select 3',5'-cyclic nucleotide phosphodiesterases exhibit altered expression in the aged rodent brain.
      Kelly, Michy P, et al.
      Cell. Signal., 26: 383-97 (2014) 2014

      Show Abstract
      24184653
      Postsynaptic density scaffold SAP102 regulates cortical synapse development through EphB and PAK signaling pathway.
      Murata, Yasunobu and Constantine-Paton, Martha
      J. Neurosci., 33: 5040-52 (2013) 2013

      Show Abstract
      23486974
      TNF-α Downregulates Inhibitory Neurotransmission through Protein Phosphatase 1-Dependent Trafficking of GABAA Receptors.
      Pribiag, Horia and Stellwagen, David
      J. Neurosci., 33: 15879-93 (2013) 2013

      Show Abstract
      24089494
      Selective loss of AMPA receptors at corticothalamic synapses in the epileptic stargazer mouse.
      Z Barad,O Shevtsova,G W Arbuthnott,B Leitch
      Neuroscience 217 2012

      Show Abstract
      22609941
      Two-stage AMPA receptor trafficking in classical conditioning and selective role for glutamate receptor subunit 4 (tGluA4) flop splice variant.
      Zheng, Zhaoqing, et al.
      J. Neurophysiol., 108: 101-11 (2012) 2012

      Show Abstract
      22490558
      Genetic Deletion of NR3A Accelerates Glutamatergic Synapse Maturation.
      Maile A Henson,Rylan S Larsen,Shelikha N Lawson,Isabel Pérez-Otaño,Nobuki Nakanishi,Stuart A Lipton,Benjamin D Philpot
      PloS one 7 2012

      Show Abstract
      22870318
      Deficits in LTP Induction by 5-HT2A Receptor Antagonist in a Mouse Model for Fragile X Syndrome.
      Zhao-Hui Xu,Qi Yang,Lan Ma,Shui-Bing Liu,Guang-Sheng Chen,Yu-Mei Wu,Xiao-Qiang Li,Gang Liu,Ming-Gao Zhao
      PloS one 7 2012

      Show Abstract
      23119095
      Distinct regional and subcellular localization of the actin-binding protein filamin a in the mature rat brain.
      Yoav Noam,Lise Phan,Shawn McClelland,Erik M Manders,Markus U Ehrengruber,Wytse J Wadman,Tallie Z Baram,Yuncai Chen
      The Journal of comparative neurology 520 2012

      Show Abstract
      22434607
      Altered glutamate receptor function in the cerebellum of the Ppt1-/- mouse, a murine model of infantile neuronal ceroid lipofuscinosis.
      Rozzy Finn,Attila D Kovács,David A Pearce
      Journal of neuroscience research 90 2012

      Show Abstract
      21971706
      Rapid enrichment of presynaptic protein in boutons undergoing classical conditioning is mediated by brain-derived neurotrophic factor.
      Li, W and Keifer, J
      Neuroscience, 203: 50-8 (2012) 2012

      Show Abstract
      22202461