AB10615 | Anti-PDEF Antibody

AB10615
100  μg
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ELISARbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number AB10615
      Brand Family Chemicon®
      Synonyms Prostate-derived Ets Factor
      Trade Name
      • Chemicon
      Description Anti-PDEF Antibody
      Background Information PDEF (prostate-derived Ets factor) is a 37.5-kDa protein that acts as an androgen-independent transcriptional activator for the PSA (prostate specific antigen) promoter. It also interacts directly with the DNA binding domain of androgen receptor and enhances androgen-mediated activation of the PSA promoter. PDEF was shown to have transcript levels 192-fold higher in the peripheral blood of some breast cancer patients in comparison with normal individuals. PDEF mRNA is also frequently over-expressed in human breast tumors as well as in human ovarian tumors. In ovarian tumors, PDEF expression seems to down-modulate malignant potential, and thus provides a rationale to screen for drugs that induce PDEF expression in epithelial ovarian tumors.
      References
      Product Information
      Format Affinity Purified
      Specificity Recognizes PDEF.
      Presentation This polyclonal antibody is supplied in 400 μL of phosphate buffered saline (pH 7.4) containing 0.1% sodium azide.
      Applications
      Application Detect PDEF using this Anti-PDEF Antibody validated for use in ELISA & WB.
      Key Applications
      • Western Blotting
      • ELISA
      Application Notes ELISA: 0.1-1μg/mL
      Western Blotting: 1-3 μg/mL
      Biological Information
      Immunogen species Synthetic peptide derived from the internal region of human PDEF (prostate-derived Ets factor).
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Rabbit
      Specificity Recognizes PDEF.
      Species Reactivity Human
      Species Reactivity Note Human (positive control: LNCaP cells)
      Antibody Type Polyclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary PDEF is an ETS transcription factor expressed in prostate epithelial cells. It acts as an androgen-independent transactivator of PSA (MIM 176820) expression.[supplied by OMIM]
      Gene Symbol
      • RP11-375E1__A.3
      • SPDEF
      • bA375E1.3
      • PSE
      • PDEF
      Purification Method Immunoaffinity purified
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: O95238 # May function as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT- 3' DNA sequences. May play a role in the regulation of the prostate gland and/or prostate cancer development. Acts as a transcriptional activator for SERPINB5 promoter.
      SIZE: 335 amino acids; 37518 Da
      SUBUNIT: Interacts with the DNA-binding domain of the androgen receptor. Interacts with NKX3-1.
      SUBCELLULAR LOCATION: Nucleus (Probable).
      TISSUE SPECIFICITY: Expressed in a very restricted set of primarily hormone-regulated epithelial tissues with particularly high expression in the prostate gland. Significantly lower expression is seen in other hormone regulated tissues such as mammary gland, salivary gland, and ovary. Expressed in prostate carcinoma cells.
      SIMILARITY: SwissProt: O95238 ## Belongs to the ETS family. & Contains 1 ETS DNA-binding domain. & Contains 1 PNT (pointed) domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Store at -20°C in undiluted aliquots for 1 year from date of shipment.
      Packaging Information
      Qty/Pck 100 µg
      Material Size 100
      Material Package μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Millipore - COA - RABBIT ANTI-PDEF POLYCLONAL ANTIBODY - 2002176 2002176

      References

      Reference overviewPub Med ID
      Prostate epithelium-derived Ets transcription factor mRNA is overexpressed in human breast tumors and is a candidate breast tumor marker and a breast tumor antigen.
      Ghadersohi, A and Sood, A K
      Clin. Cancer Res., 7: 2731-8 (2001) 2001

      Show Abstract
      11555586
      PDEF, a novel prostate epithelium-specific ets transcription factor, interacts with the androgen receptor and activates prostate-specific antigen gene expression.
      Oettgen, P, et al.
      J. Biol. Chem., 275: 1216-25 (2000) 2000

      Show Abstract
      10625666

      Data Sheet

      Title
      Anti-PDEF - Data Sheet