48-613MAG | MILLIPLEX MAP EGFR Profiler Magnetic Bead 8-Plex Kit - Cell Signaling Multiplex Assay

EGFR Profiler Magnetic Bead 8-Plex Analysis of Untreated HeLa Cell Lysates vs. Stimulated A431 Cell Lysates
EGFR Profiler Magnetic Bead 8-Plex Analysis of Untreated HeLa Cell Lysates vs. Stimulated A431 C
48-613MAG
1  96-well plate
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      Overview

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      EGFR (Total), EGFR (Thr654), EGFR (Thr669), EGFR (Tyr845), EGFR (Tyr1045), EGFR (Tyr1068), EGFR (Tyr1086), EGFR (Ser1047)HMplexLuminex xMAP
      Description
      Catalogue Number 48-613MAG
      Trade Name
      • MILLIPLEX
      Description MILLIPLEX MAP EGFR Profiler Magnetic Bead 8-Plex Kit - Cell Signaling Multiplex Assay
      Overview Cells respond to their environment in many different ways through intracellular signaling. Signaling through Receptor Tyrosine Kinases (RTKs) often promote increased metabolism and cell growth. Epidermal Growth Factor Receptor (EGFR) is a 175 kDa receptor activated by such ligands as EGF and heregulin. EGF binding induces EGFR autophosphorylation at specific tyrosine residues, promoting the docking of several SH2 domain-containing adaptor proteins to EGFR that, in turn, activate downstream signaling pathways such as MAPK, AKT and STAT. In addition to autophosphorylated tyrosine residues, EGFR contains other phosphorylation sites including phosphothreonine and phosphoserine residues that are involved in crosstalk with other signaling pathways and EGFR down regulation.
      References
      Product Information
      Detection method Luminex xMAP
      Specificity Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Configuration Premixed
      Precision (%)
      • Intra-Assay (Control Peptide): 4-18
        EGFR total is N/A
      • Intra-Assay (A431): 2-8
      • Intra-Assay (Control Peptide): 2-10
        EGFR total is N/A
      • Inter-Assay (A431): 7-13
      Panel Type Cell Signaling
      Applications
      Application The analytes available for this multiplex kit are: EGFR (Thr654), EGFR (Thr669), EGFR (Tyr845), EGFR (Tyr1045), EGFR (Tyr1068), EGFR (Tyr1086), EGFR (Ser1047), EGFR (Total).
      Key Applications
      • Multiplexing
      Application Notes • An overnight (4°C) incubation is recommended for best results.
      • This assay requires 25 µL diluted cell lysate per well.
      • This kit must be run using Assay Buffer 1 (provided).
      • 1 - 25 µg cell lysate/well (recommended starting concentration is 40 to 1,000 µg protein/mL).
      Biological Information
      Specificity Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Species Reactivity Human
      Analytes Available
      • EGFR (Total)
      • EGFR (Thr654)
      • EGFR (Thr669)
      • EGFR (Tyr845)
      • EGFR (Tyr1045)
      • EGFR (Tyr1068)
      • EGFR (Tyr1086)
      • EGFR (Ser1047)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Recommended storage for kit components is 2 - 8°C.
      Packaging Information
      Packaging 96-well plate
      Qty/Pck 96-well plate
      Material Size 1
      Material Package 96-well plate
      Transport Information
      Supplemental Information
      Specifications