21-170 | TOPflash (TCF Reporter Plasmid)

21-170
5 µg  
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      Overview

      Description
      Catalogue Number 21-170
      Brand Family Upstate
      Trade Name
      • Upstate
      Description TOPflash (TCF Reporter Plasmid)
      References
      Product Information
      Applications
      Application Transfection grade T-cell factor reporter plasmid containing 2 sets (with
      the second set in the reverse orientation) of 3 copies of the TCF binding site (wild type) upstream of the Thymidine Kinase minimal promoter and
      Luciferase open reading frame.
      Key Applications
      • Transfection
      Biological Information
      Entrez Gene Number
      Entrez Gene Summary This gene encodes transcription factor 4, a basic helix-turn-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is expressed predominantly in pre-B-cells, although it is found in other tissues as well. Multiple alternatively spliced transcript variants that encode different proteins have been described.
      Gene Symbol
      • HNF1a
      • TCF1
      • MODY3
      • HNF1
      • HNF-1A
      • TCF-1
      • HNF1A
      • LFB1
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: P20823 # Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
      SIZE: 631 amino acids; 67356 Da
      SUBUNIT: Binds DNA as a dimer.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Liver.
      DISEASE: SwissProt: P20823 # Defects in HNF1A may predispose to hepatic adenomas [MIM:142330]. Hepatic adenomas are benign tumors at risk of malignant transformation. Bi-allelic inactivation of HNF1A, whether sporadic or associated with MODY3, may be an early step in the developmant of some hepatocellular carcinomas. & Defects in HNF1A are the cause of maturity onset diabetes of the young type 3 (MODY3) [MIM:600496]; also symbolized MODY-3. MODY [MIM:606391] is a form of diabetes characterized by an autosomal dominant mode of inheritance, age of onset of 25 years or younger and a primary defect in insulin secretion. The clinical phenotype of MODY3 is characterized by severe insulin secretory defects, and by major hyperglycemia associated with microvascular complications. & Defects in HNF1A are a cause of susceptibility to insulin-dependent diabetes mellitus (IDDM) [MIM:222100].
      SIMILARITY: SwissProt: P20823 ## Belongs to the HNF1 homeobox family. & Contains 1 homeobox DNA-binding domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size 5 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      TOPflash (TCF Reporter Plasmid) - 18908 18908
      TOPflash (TCF Reporter Plasmid) - 19491 19491
      TOPflash (TCF Reporter Plasmid) - 19963 19963
      TOPflash (TCF Reporter Plasmid) - 20756 20756
      TOPflash (TCF Reporter Plasmid) - 23206 23206
      TOPflash (TCF Reporter Plasmid) - 26217 26217
      TOPflash (TCF Reporter Plasmid) - 27752 27752
      TOPflash (TCF Reporter Plasmid) - DAM1560242 DAM1560242
      TOPflash (TCF Reporter Plasmid) - DAM1631964 DAM1631964
      TOPflash (TCF Reporter Plasmid) - DAM1662092 DAM1662092
      TOPflash (TCF Reporter Plasmid) - DAM1765545 DAM1765545
      TOPflash (TCF Reporter Plasmid) - JBC1367022 JBC1367022
      TOPflash (TCF Reporter Plasmid) - JBC1888227 JBC1888227

      References

      Reference overviewApplicationPub Med ID
      Histone deacetylase 7 controls endothelial cell growth through modulation of beta-catenin.
      Margariti, Andriana, et al.
      Circ. Res., 106: 1202-11 (2010) 2010

      Show Abstract
      20224040
      Murine Frizzled-1 behaves as an antagonist of the canonical Wnt/beta-catenin signaling
      Roman-Roman, S., et al
      J Biol Chem, 279:5725-33 (2004) 2004

      14627707
      Characterization and functional analysis of the murine Frat2 gene
      van Amerongen, R., et al
      J Biol Chem, 279:26967-74 (2004) 2004

      15073180
      Adenomatous Polyposis Coli (APC)-independent Regulation of {beta}-Catenin Degradation via a Retinoid X Receptor-mediated Pathway
      Xiao, J. H., et al
      J Biol Chem, 278:29954-29962 (2003) 2003

      Transfection12771132
      The TAK1-NLK-MAPK-related pathway antagonizes signalling between beta-catenin and transcription factor TCF.
      Ishitani, T, et al.
      Nature, 399: 798-802 (1999) 1999

      Show Abstract
      10391247
      Two members of the Tcf family implicated in Wnt/beta-catenin signaling during embryogenesis in the mouse.
      Korinek, V, et al.
      Mol. Cell. Biol., 18: 1248-56 (1998) 1998

      Show Abstract
      9488439
      The Xenopus Wnt effector XTcf-3 interacts with Groucho-related transcriptional repressors.
      Roose, J, et al.
      Nature, 395: 608-12 (1998) 1998

      Show Abstract
      9783587
      Armadillo coactivates transcription driven by the product of the Drosophila segment polarity gene dTCF
      van de Wetering, M, et al
      Cell, 88:789-99 (1997) 1997

      9118222
      Species differences in kidney necrosis and DNA damage, distribution and glutathione-dependent metabolism of 1,2-dibromo-3-chloropropane (DBCP).
      E J Søderlund,M Låg,J A Holme,G Brunborg,J G Omichinski,J E Dahl,S D Nelson,E Dybing
      Pharmacology & toxicology 66 1990

      Show Abstract
      2371234

      Technical Info

      Title
      TOPflash (TCF Reporter Plasmid) - Nucleotide Sequence File