17-218 | Ras Activation Assay Kit

Affinity Precipitation/Immunoblot Analysis: 1 milligram of HeLa cell lysate was loaded with GDP (lane 1) or with GTPγS (lane 2) then incubated with Raf-1 RBD, agarose. Subsequent immunoblotting was performed with 1μg/ml of Anti-Ras. Arrow indicates Ras (~21kDa).
Affinity Precipitation/Immunoblot Analysis: 1 milligram of HeLa cell lysate was loaded with GDP (lan
17-218
1 kit  Kit capacity: 30 assays
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      Overview

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      Key Spec Table

      Key Applications
      EA
      Description
      Catalogue Number 17-218
      Brand Family Upstate
      Trade Name
      • Upstate
      Description Ras Activation Assay Kit
      References
      Product Information
      Components
      • Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
      • Ras Assay Reagent (Raf-1 RBD, agarose) (Cat.# 14-278)
      • Anti-Ras, clone RAS10 (Cat.# 05-516)
      • 100X GTPγS, 10mM (Cat.# 20-176)
      • 100X GDP, 100mM (Cat.# 20-177)
      Applications
      Key Applications
      • Enzyme Assay
      Biological Information
      Entrez Gene Number
      Entrez Gene Summary Members of the RAS superfamily of GTP-binding proteins, which includes MRAS, are membrane-anchored, intracellular signal transducers responsible for a variety of normal cellular functions. They are oncogenically activated in a significant fraction of tumors.[supplied by OMIM]
      Gene Symbol
      • RRAS3
      • MRAS
      • M-RAs
      • M-RAS
      • FLJ42964
      • R-RAS3
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: O14807 # May serve as an important signal transducer for a novel upstream stimuli in controlling cell proliferation. Weakly activates the MAP kinase pathway.
      SIZE: 208 amino acids; 23846 Da
      SUBUNIT: Interacts with RGL3 (By similarity).
      SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential).
      TISSUE SPECIFICITY: Expression highly restricted to the brain and heart.
      SIMILARITY: SwissProt: O14807 ## Belongs to the small GTPase superfamily. Ras family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance This kit precipitated Ras-GTP from HeLa cell lysates that had been loaded with GTPγS. The precipitated Ras-GTP was detected by western immunoblot using 1μg/ml anti-Ras, clone RAS10
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size 1 kit
      Material Package Kit capacity: 30 assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Languages
      English

      Certificates of Analysis

      TitleLot Number
      Ras Activation Assay Kit (non-radioactive) - 2390546 2390546
      Ras Activation Assay Kit (non-radioactive) - 1959294 1959294
      Ras Activation Assay Kit (non-radioactive) - 2166054 2166054
      Ras Activation Assay Kit (non-radioactive) - 2210394 2210394
      Ras Activation Assay Kit (non-radioactive) - 2281660 2281660
      Ras Activation Assay Kit (non-radioactive) - 2329806 2329806
      Ras Activation Assay Kit (non-radioactive) -2484190 2484190
      Ras Activation Assay Kit - 2430405 2430405
      Ras Activation Assay Kit - 0606032766 0606032766
      Ras Activation Assay Kit - 0609039663 0609039663
      Ras Activation Assay Kit - 0611045665 0611045665
      Ras Activation Assay Kit - 16849 16849
      Ras Activation Assay Kit - 18775 18775
      Ras Activation Assay Kit - 19032 19032
      Ras Activation Assay Kit - 19318 19318
      Ras Activation Assay Kit - 19683 19683
      Ras Activation Assay Kit - 2047789 2047789
      Ras Activation Assay Kit - 2064532 2064532
      Ras Activation Assay Kit - 20653 20653
      Ras Activation Assay Kit - 21074 21074
      Ras Activation Assay Kit - 21375 21375
      Ras Activation Assay Kit - 21955 21955
      Ras Activation Assay Kit - 22838 22838
      Ras Activation Assay Kit - 23501 23501
      Ras Activation Assay Kit - 25027 25027
      Ras Activation Assay Kit - 26220 26220
      Ras Activation Assay Kit - 29904 29904
      Ras Activation Assay Kit - 32019 32019
      Ras Activation Assay Kit - 33439 33439
      Ras Activation Assay Kit - DAM1503342 DAM1503342
      Ras Activation Assay Kit - DAM1556075 DAM1556075
      Ras Activation Assay Kit - DAM1567201 DAM1567201
      Ras Activation Assay Kit - DAM1601770 DAM1601770
      Ras Activation Assay Kit - DAM1692676 DAM1692676
      Ras Activation Assay Kit - DAM1745418 DAM1745418
      Ras Activation Assay Kit - DAM1792809 DAM1792809
      Ras Activation Assay Kit - JBC1370020 JBC1370020
      Ras Activation Assay Kit - JBC1393242 JBC1393242
      Ras Activation Assay Kit - JBC1831438 JBC1831438
      Ras Activation Assay Kit - JBC1903640 JBC1903640
      Ras Activation Assay Kit -2500472 2500472

      References | 24 Available | See All References

      Reference overviewApplicationPub Med ID
      The TGFβ Receptor-interacting Protein km23-1/DYNLRB1 Plays an Adaptor Role in TGFβ1 Autoinduction via Its Association with Ras.
      Qunyan Jin,Wei Ding,Kathleen M Mulder
      The Journal of biological chemistry 287 2012

      Show Abstract
      22637579
      Effects of estrogen on stress-induced premature senescence of vascular smooth muscle cells: A novel mechanism for the \time window theory\ of menopausal hormone therapy.
      Zhu C, Zhang L, Zheng Y, Xu J, Song J, Rolfe BE, Campbell JH
      Atherosclerosis 215 294-300. Epub 2011 Jan 19. 2011

      21300357
      E4orf1: a novel ligand that improves glucose disposal in cell culture.
      Emily J Dhurandhar,Olga Dubuisson,Nazar Mashtalir,Rashmi Krishnapuram,Vijay Hegde,Nikhil V Dhurandhar
      PloS one 6 2011

      Show Abstract Full Text Article
      21886789
      Ligand-specific function of transforming growth factor beta in epithelial-mesenchymal transition in heart development.
      Mohamad Azhar,Raymond B Runyan,Connie Gard,L Philip Sanford,Marian L Miller,Anastasia Andringa,Sharon Pawlowski,Sudarsan Rajan,Thomas Doetschman
      Developmental dynamics : an official publication of the American Association of Anatomists 238 2009

      Show Abstract Full Text Article
      19161227
      Gene silencing for epidermal growth factor receptor variant III induces cell-specific cytotoxicity.
      Yamoutpour, Farnaz, et al.
      Mol. Cancer Ther., 7: 3586-97 (2008) 2008

      Show Abstract
      19001441
      Activated K-RAS increases polyamine uptake in human colon cancer cells through modulation of caveolar endocytosis.
      Upal K Basu Roy,Nathaniel S Rial,Karen L Kachel,Eugene W Gerner
      Molecular carcinogenesis 47 2008

      Show Abstract Full Text Article
      18176934
      RIG1 suppresses Ras activation and induces cellular apoptosis at the Golgi apparatus.
      Tsai, Fu-Ming, et al.
      Cell. Signal., 19: 989-99 (2007) 2007

      Show Abstract
      17196792
      RIG1 inhibits the Ras/mitogen-activated protein kinase pathway by suppressing the activation of Ras.
      Tsai, Fu-Ming, et al.
      Cell. Signal., 18: 349-58 (2006) 2006

      Show Abstract
      16005186
      Methylation and inhibition of expression of uPA by the RAS oncogene: divergence of growth control and invasion in breast cancer cells.
      Pouya Pakneshan, Moshe Szyf, Shafaat A Rabbani
      Carcinogenesis 26 557-64 2005

      Show Abstract
      15618232
      Optimization of a nonradioactive method for consistent and sensitive determination of activated K-ras protein.
      Richard J Calvert,Wafa Kammouni,Keith D Kikawa
      Analytical biochemistry 343 2005

      Show Abstract
      16018961

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      Kit Component

       Catalogue Number Description  
      05-516 Anti-Ras Antibody, clone RAS10 Show Pricing & Availability
      14-278 Ras Assay Reagent (Raf-1 RBD, agarose) Show Pricing & Availability
      20-168 Mg2+ Lysis/Wash Buffer, 5X Show Pricing & Availability
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      Product Families

      RAS Antibodies, Kits, Proteins & Plasmids

      Millipore’s RAS antibodies, kits, and plasmids are high quality, validated tools for research. These are based on the expertise of Upstate & Chemicon. See below for Millipore’s Ras G-protein products. Learn More >>

      Categories

      Life Science Research > Protein Detection and Quantification > Activity Assays > Activation Assays