17-218 | Ras Activation Assay Kit

Affinity Precipitation/Immunoblot Analysis: 1 milligram of HeLa cell lysate was loaded with GDP (lane 1) or with GTPγS (lane 2) then incubated with Raf-1 RBD, agarose. Subsequent immunoblotting was performed with 1μg/ml of Anti-Ras. Arrow indicates Ras (~21kDa).
Affinity Precipitation/Immunoblot Analysis: 1 milligram of HeLa cell lysate was loaded with GDP (lan
1 kit  Kit capacity: 30 assays
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      Replacement Information

      Key Spec Table

      Key Applications
      Catalogue Number 17-218
      Replaces SGT435
      Brand Family Upstate
      Trade Name
      • Upstate
      Description Ras Activation Assay Kit
      Product Information
      • Ras Assay Reagent (Raf-1 RBD, agarose) (Cat.# 14-278)
      • Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
      • 100X GDP, 100mM (Cat.# 20-177)
      • 100X GTPγS, 10mM (Cat.# 20-176)
      • Anti-Ras, clone RAS10 (Cat.# 05-516)
      Key Applications
      • Enzyme Assay
      Biological Information
      Entrez Gene Number
      Entrez Gene Summary Members of the RAS superfamily of GTP-binding proteins, which includes MRAS, are membrane-anchored, intracellular signal transducers responsible for a variety of normal cellular functions. They are oncogenically activated in a significant fraction of tumors.[supplied by OMIM]
      Gene Symbol
      • MRAS
      • RRAS3
      • R-RAS3
      • FLJ42964
      • M-RAS
      • M-RAs
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: O14807 # May serve as an important signal transducer for a novel upstream stimuli in controlling cell proliferation. Weakly activates the MAP kinase pathway.
      SIZE: 208 amino acids; 23846 Da
      SUBUNIT: Interacts with RGL3 (By similarity).
      SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential).
      TISSUE SPECIFICITY: Expression highly restricted to the brain and heart.
      SIMILARITY: SwissProt: O14807 ## Belongs to the small GTPase superfamily. Ras family.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance This kit precipitated Ras-GTP from HeLa cell lysates that had been loaded with GTPγS. The precipitated Ras-GTP was detected by western immunoblot using 1μg/ml anti-Ras, clone RAS10
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size 1 kit
      Material Package Kit capacity: 30 assays
      Transport Information
      Supplemental Information




      Certificates of Analysis

      TitleLot Number
      Ras Activation Assay Kit (non-radioactive) - 23905462390546
      Ras Activation Assay Kit (non-radioactive) - 19592941959294
      Ras Activation Assay Kit (non-radioactive) - 21660542166054
      Ras Activation Assay Kit (non-radioactive) - 22103942210394
      Ras Activation Assay Kit (non-radioactive) - 22816602281660
      Ras Activation Assay Kit (non-radioactive) - 23298062329806
      Ras Activation Assay Kit (non-radioactive) -24841902484190
      Ras Activation Assay Kit - 24304052430405
      Ras Activation Assay Kit - 06060327660606032766
      Ras Activation Assay Kit - 06090396630609039663
      Ras Activation Assay Kit - 06110456650611045665
      Ras Activation Assay Kit - 1684916849
      Ras Activation Assay Kit - 1877518775
      Ras Activation Assay Kit - 1903219032
      Ras Activation Assay Kit - 1931819318
      Ras Activation Assay Kit - 1968319683
      Ras Activation Assay Kit - 20477892047789
      Ras Activation Assay Kit - 20645322064532
      Ras Activation Assay Kit - 2065320653
      Ras Activation Assay Kit - 2107421074
      Ras Activation Assay Kit - 2137521375
      Ras Activation Assay Kit - 2195521955
      Ras Activation Assay Kit - 2283822838
      Ras Activation Assay Kit - 2350123501
      Ras Activation Assay Kit - 2502725027
      Ras Activation Assay Kit - 25505712550571
      Ras Activation Assay Kit - 2622026220
      Ras Activation Assay Kit - 2990429904
      Ras Activation Assay Kit - 3201932019
      Ras Activation Assay Kit - 3343933439
      Ras Activation Assay Kit - DAM1503342DAM1503342
      Ras Activation Assay Kit - DAM1556075DAM1556075
      Ras Activation Assay Kit - DAM1567201DAM1567201
      Ras Activation Assay Kit - DAM1601770DAM1601770
      Ras Activation Assay Kit - DAM1692676DAM1692676
      Ras Activation Assay Kit - DAM1745418DAM1745418
      Ras Activation Assay Kit - DAM1792809DAM1792809
      Ras Activation Assay Kit - JBC1370020JBC1370020
      Ras Activation Assay Kit - JBC1393242JBC1393242
      Ras Activation Assay Kit - JBC1831438JBC1831438
      Ras Activation Assay Kit - JBC1903640JBC1903640
      Ras Activation Assay Kit -25004722500472

      References | 24 Available | See All References

      Reference overviewApplicationPub Med ID
      The TGFβ Receptor-interacting Protein km23-1/DYNLRB1 Plays an Adaptor Role in TGFβ1 Autoinduction via Its Association with Ras.
      Qunyan Jin,Wei Ding,Kathleen M Mulder
      The Journal of biological chemistry 287 2012

      Show Abstract
      Effects of estrogen on stress-induced premature senescence of vascular smooth muscle cells: A novel mechanism for the \time window theory\ of menopausal hormone therapy.
      Zhu C, Zhang L, Zheng Y, Xu J, Song J, Rolfe BE, Campbell JH
      Atherosclerosis 215 294-300. Epub 2011 Jan 19. 2011

      E4orf1: a novel ligand that improves glucose disposal in cell culture.
      Emily J Dhurandhar,Olga Dubuisson,Nazar Mashtalir,Rashmi Krishnapuram,Vijay Hegde,Nikhil V Dhurandhar
      PloS one 6 2011

      Show Abstract Full Text Article
      Ligand-specific function of transforming growth factor beta in epithelial-mesenchymal transition in heart development.
      Mohamad Azhar,Raymond B Runyan,Connie Gard,L Philip Sanford,Marian L Miller,Anastasia Andringa,Sharon Pawlowski,Sudarsan Rajan,Thomas Doetschman
      Developmental dynamics : an official publication of the American Association of Anatomists 238 2009

      Show Abstract Full Text Article
      Gene silencing for epidermal growth factor receptor variant III induces cell-specific cytotoxicity.
      Yamoutpour, Farnaz, et al.
      Mol. Cancer Ther., 7: 3586-97 (2008) 2008

      Show Abstract
      Activated K-RAS increases polyamine uptake in human colon cancer cells through modulation of caveolar endocytosis.
      Upal K Basu Roy,Nathaniel S Rial,Karen L Kachel,Eugene W Gerner
      Molecular carcinogenesis 47 2008

      Show Abstract Full Text Article
      RIG1 suppresses Ras activation and induces cellular apoptosis at the Golgi apparatus.
      Tsai, Fu-Ming, et al.
      Cell. Signal., 19: 989-99 (2007) 2007

      Show Abstract
      RIG1 inhibits the Ras/mitogen-activated protein kinase pathway by suppressing the activation of Ras.
      Tsai, Fu-Ming, et al.
      Cell. Signal., 18: 349-58 (2006) 2006

      Show Abstract
      Methylation and inhibition of expression of uPA by the RAS oncogene: divergence of growth control and invasion in breast cancer cells.
      Pouya Pakneshan, Moshe Szyf, Shafaat A Rabbani
      Carcinogenesis 26 557-64 2005

      Show Abstract
      Optimization of a nonradioactive method for consistent and sensitive determination of activated K-ras protein.
      Richard J Calvert,Wafa Kammouni,Keith D Kikawa
      Analytical biochemistry 343 2005

      Show Abstract


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      20-176 100X GTPγS, 10mM Show Pricing & Availability
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      Product Families


      Life Science Research > Protein Detection and Quantification > Activity Assays > Activation Assays