17-192 | MAP Kinase/Erk Immunoprecipitation Kinase Assay Kit, non-radioactive

17-192
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      Overview

      Key Spec Table

      Key ApplicationsAnalytes Available
      KAMAP Kinase/Erk
      Description
      Catalogue Number 17-192
      Brand Family Upstate
      Trade Name
      • Upstate
      Description MAP Kinase/Erk Immunoprecipitation Kinase Assay Kit, non-radioactive
      References
      Product Information
      Components Magnesium/ATP Cocktail (Cat.# 20-113) Assay Dilution Buffer, 5X (Cat.# 20-145) Anti-MAP Kinase 1/2 (Erk1/2), agarose (Cat.# 16-111) Anti-phospho-MBP, clone P12 (Cat.# 05-429) MAP Kinase/Erk Substrate Cocktail II (Cat.# 20-166) Inhibitor Cocktail (Cat.# 20-116)
      Applications
      Application MAP Kinase/Erk Immunoprecipitation Kinase Assay Kit is designed to measure phosphotransferase activity in an immunocomplex formed between the MAP Kinase R2 antibody & MAP Kinase (p44mpk).
      Key Applications
      • Kinase Assay
      Biological Information
      Analytes Available
      • MAP Kinase/Erk
      Entrez Gene Number
      Entrez Gene Summary The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
      Gene Symbol
      • ERK2
      • MAPK1
      • p40
      • PRKM2
      • MAPK2
      • PRKM1
      • p42-MAPK
      • p38
      • p41
      • ERK
      • ERK-2
      • ERT1
      • P42MAPK
      • p41mapk
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: P28482 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
      COFACTOR: Magnesium (By similarity).
      SIZE: 360 amino acids; 41390 Da
      SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine- kinase activity. Interacts with HSF4.
      DOMAIN: SwissProt: P28482 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
      PTM: Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme.
      SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance The assay kit is designed to measure phosphotransferase activity in an immunocomplex formed between the MAP Kinase R2 antibody and MAP Kinase (p44mpk). This precipitated enzyme is used to phosphorylate a specific substrate, myelin basic protein (MBP).
      Sales Restrictions This product is derived from bovine source. Export of this product to certain countries may be restricted. Please contact Customer Service or your local distributor to inquire about product availability and export options.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size 1 kit
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      MAP Kinase/Erk Immunoprecipitation Kinase Assay Kit 2439207
      MAP Kinase/Erk Immunoprecipitation Kinase Assay Kit - 2043451 2043451
      MAP Kinase/Erk Immunoprecipitation Kinase Assay Kit - DAM1723427 DAM1723427
      MAP Kinase/Erk Immunoprecipitation Kinase Assay Kit - DAM1821251 DAM1821251
      MAP Kinase/Erk Immunoprecipitation Kinase Assay Kit - JBC1852342 JBC1852342
      MAP Kinase/Erk Immunoprecipitation Kinase Assay Kit - JBC1878094 JBC1878094
      MAP Kinase/Erk Immunoprecipitation Kinase Assay Kit - NG1623621 NG1623621
      MAP Kinase/Erk Immunoprecipitation Kinase Assay Kit, non-radioactive - 16118 16118
      MAP Kinase/Erk Immunoprecipitation Kinase Assay Kit, non-radioactive - 17702 17702
      MAP Kinase/Erk Immunoprecipitation Kinase Assay Kit, non-radioactive - 18181 18181
      MAP Kinase/Erk Immunoprecipitation Kinase Assay Kit, non-radioactive - 19177 19177
      MAP Kinase/Erk Immunoprecipitation Kinase Assay Kit, non-radioactive - 21053 21053
      MAP Kinase/Erk Immunoprecipitation Kinase Assay Kit, non-radioactive - 21653 21653
      MAP Kinase/Erk Immunoprecipitation Kinase Assay Kit, non-radioactive - 33523 33523

      Related Products & Applications

      Kit Component

       Catalogue Number Description  
      05-429 Anti-phospho-MBP Antibody, clone P12 Show Pricing & Availability
      16-111 Anti-MAP Kinase 1/2 (Erk1/2) Antibody, agarose Show Pricing & Availability
      20-113 Magnesium/ATP Cocktail Show Pricing & Availability
      20-116 Kinase Inhibitor Cocktail Show Pricing & Availability
      20-145 Assay Dilution Buffer, 5X Show Pricing & Availability

      Product Families

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      Categories

      Life Science Research > Protein Detection and Quantification > Activity Assays > Kinase Assays