16-251 | Anti-phospho-Histone H2B (Ser14) Antibody, clone MC603, FITC conjugate, rabbit monoclonal

Left panel: HL60 cells stained with anti-phospho-Histone H2B (Ser14), clone MC603, FITC conjugate after overnight serum starvation, negative control.
Middle panel: HL60 cells stained with anti-phospho-Histone H2B (Ser14), clone MC603, FITC conjugate after overnight serum starvation followed by treatment with 40μg/ml Etoposide for 2hrs at 37°C.
Right panel: HL60 cells stained with anti-phospho-Histone H2B (Ser14), clone MC603, FITC conjugate after overnight serum starvation followed by treatment with 40μg/ml Etoposide for 4hrs at 37°C.
Left panel: HL60 cells stained with anti-phospho-Histone H2B (Ser14), clone MC603, FITC conju
16-251
50 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFCRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number 16-251
      Brand Family Upstate
      Synonyms H2BS14P Histone H2B (phospho S14)
      Trade Name
      • Upstate
      Description Anti-phospho-Histone H2B (Ser14) Antibody, clone MC603, FITC conjugate, rabbit monoclonal
      References
      Product Information
      Format Purified
      Specificity phospho-histone H2B (Ser14)
      Applications
      Application Anti-phospho-Histone H2B (Ser14) Antibody, clone MC603, FITC conjugate is a rabbit monoclonal antibody for detection of phospho-Histone H2B (Ser14) also known as H2BS14P, Histone H2B (phospho S14) & has been validated in FC.
      Key Applications
      • Flow Cytometry
      Biological Information
      Immunogen species KLH-conjugated synthetic peptide containing the sequence KGpSK in which pS corresponds to phospho-serine 14 of human Histone H2B. Clone MC603.
      Clone MC603
      Host Rabbit
      Specificity phospho-histone H2B (Ser14)
      Isotype IgG
      Species Reactivity Human
      Species Reactivity Note Broad species cross-reactivity expected based on sequence homology.
      Antibody Type Monoclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene contain a palindromic termination element.
      Gene Symbol
      • HIST3H2BB
      Modifications
      • Phosphorylation
      Purification Method Protein A purfied
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: Q8N257 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 126 amino acids; 13908 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. & Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class- switch recombination.
      SIMILARITY: SwissProt: Q8N257 ## Belongs to the histone H2B family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Routinely evaluated by flow cytometry.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions 2 years at 4°C from date of shipment
      Packaging Information
      Material Size 50 µg
      Transport Information
      Supplemental Information
      Specifications