07-789 | Anti-phospho-AKT (Thr34) Antibody

Immunoblot Analysis Lysates from untreated (lane 1) and Calyculin A treated (100nM for 30 min.) (lane 2) A431 cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-phospho-AKT (Thr34) for 2 hours at room temperature. Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-AKT (Thr 34).
Immunoblot Analysis Lysates from untreated (lane 1) and Calyculin A treated (100nM for 30 min
07-789
100  µL
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number 07-789
      Brand Family Upstate
      Synonyms Akt1, PKB, PRKBA, RAC, RAC-ALPHA
      Trade Name
      • Upstate
      Description Anti-phospho-AKT (Thr34) Antibody
      References
      Product Information
      Format Affinity Purified
      Specificity Recognizes phospho-AKT (Thr34)
      Presentation 100 μL of epitope specific affinity purified rabbit polyclonal IgG in 50% affinity storage buffer (PBS containing 1.0 mg/mL BSA and 0.05% sodium azide) and 50% glycerol.
      Applications
      Application Anti-phospho-AKT (Thr34) Antibody detects level of phospho-AKT (Thr34) & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      Immunogen species KLH conjugated peptide corresponding to amino acid region encompassing the human phospho-AKT (Thr34); Sequence highly conserved in mouse and rat.
      Host Rabbit
      Specificity Recognizes phospho-AKT (Thr34)
      Isotype IgG
      Species Reactivity Human
      Species Reactivity Note Predicted to cross react with Mouse and Rat due to high sequence homology, but have not been tested.
      Antibody Type Polyclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
      Gene Symbol
      • AKT
      • AKT1
      • RAC
      • PRKBA
      • MGC99656
      • RAC-ALPHA
      • RAC-PK-alpha
      • C-AKT
      • PKB
      Modifications
      • Phosphorylation
      Purification Method Immunoaffinity purified
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
      SIZE: 480 amino acids; 55686 Da
      SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).
      TISSUE SPECIFICITY: In all human cell types so far analyzed.
      DOMAIN: SwissProt: P31749 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
      PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).
      SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Routinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Qty/Pck 100 µL
      Material Size 100
      Material Package µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-AKT (Thr34) (Affinity purified Rabbit polyclonal IgG) - 2442549 2442549
      Millipore - COA - Anti-phospho-AKT (Thr34) (Affinity purified Rabbit polyclonal IgG) - DAM1467540 DAM1467540
      Millipore - COAs - Anti-phospho-AKT (Thr34) - 0603026241 0603026241

      References

      Reference overviewApplicationPub Med ID
      Progesterone receptor enhances breast cancer cell motility and invasion via extranuclear activation of focal adhesion kinase.
      Fu XD, Goglia L, Sanchez AM, Flamini M, Giretti MS, Tosi V, Genazzani AR, Simoncini T
      Endocr Relat Cancer 17 431-43. Print 2010. 2010

      Show Abstract
      20233709
      Extra-nuclear signaling of progesterone receptor to breast cancer cell movement and invasion through the actin cytoskeleton.
      Xiao-Dong Fu, Maria S Giretti, Chiara Baldacci, Silvia Garibaldi, Marina Flamini, Angel Matias Sanchez, Angiolo Gadducci, Andrea R Genazzani, Tommaso Simoncini
      PloS one 3 e2790 2008

      Show Abstract Full Text Article
      Immunoblotting (Western)18665217
      Cisplatin activates Akt in small cell lung cancer cells and attenuates apoptosis by survivin upregulation
      Belyanskaya, Larisa L, et al
      Int J Cancer, 117:755-63 (2005) 2005

      15981204
      Ceramide disables 3-phosphoinositide binding to the pleckstrin homology domain of protein kinase B (PKB)/Akt by a PKCzeta-dependent mechanism
      Powell, Darren J, et al
      Mol Cell Biol, 23:7794-808 (2003) 2003

      14560023
      PKB/Akt: connecting phosphoinositide 3-kinase to cell survival and beyond.
      Marte, B M and Downward, J
      Trends Biochem. Sci., 22: 355-8 (1997) 1997

      Show Abstract
      9301337