07-449 | Anti-trimethyl-Histone H3 (Lys27) Antibody

Dot Blot Analysis:
Representative lot data.
Histone peptides with various modifications (see table) were probed with Anti-trimethyl Histone H3 (Lys27) (1:500 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.
C11: KAAR[trimethyl-K]SAPA-C
E10: KAAR[trimethyl-K]SAPSTGGVKKC
Dot Blot Analysis:
Representative lot data.
Histone peptides with various modificat
07-449
200 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HChIP-seq, ICC, IP, Mplex, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number 07-449
      Brand Family Upstate
      Synonyms H3k27me3 H3 histone family, member T histone 3, H3 histone cluster 3, H3
      Trade Name
      • Upstate
      Description Anti-trimethyl-Histone H3 (Lys27) Antibody
      Background Information Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      References
      Product Information
      Format Purified
      Specificity Trimethylated histone H3 (Lys27). Broad species cross-reactivity expected.
      Control
      • HeLa whole cell lysate.
      Presentation Purified rabbit IgG in buffer containing 0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide before the addition of 30% glycerol.
      Applications
      Application Use Anti-trimethyl-Histone H3 (Lys27) Antibody (Rabbit Polyclonal Antibody) validated in ICC, IP, Mplex, WB, ChIP-seq to detect trimethyl-Histone H3 (Lys27) also known as H3k27me3.
      Key Applications
      • ChIP-seq
      • Immunocytochemistry
      • Immunoprecipitation
      • Multiplexing
      • Western Blotting
      Application Notes Immunocytochemistry:

      Immunoprecipitation:

      Beadlyte® Histone-Peptide Specificity Assay:
      Biological Information
      Immunogen species KLH-conjugated, synthetic 2X-branched peptide containing the sequence …AR(me3K)SAP… in which me3K corresponds to trimethyl-lysine at residue 27 of human histone H3.
      Epitope Lys27
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Rabbit
      Specificity Trimethylated histone H3 (Lys27). Broad species cross-reactivity expected.
      Isotype IgG
      Species Reactivity Mouse Human
      Species Reactivity Note Human, mouse. Broad species cross-reactivity expected.
      Antibody Type Polyclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • MGC126886
      • HIST3H3
      • H3FT
      • H3/t
      • H3t
      • MGC126888
      • H3/g
      • H3.4
      • H3T
      Modifications
      • Methylation
      Purification Method Protein A purfied
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Expressed in testicular cells.
      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Routinely evaluated by western blot in acid extracted proteins from HeLa cells.

      Western Blot Analysis:
      0.4 µg/mL of anti-trimethyl histone H3 Lys27 was used on recombinant Histone H3 and HeLa cell acid extracts.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at -20°C from date of receipt.
      Packaging Information
      Material Size 200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Languages
      Deutschland

      Certificates of Analysis

      TitleLot Number
      Anti-Trimethyl-Histone H3 (Lys27) 2475696
      Anti-Trimethyl-Histone H3 (Lys27) - 2382150 2382150
      Anti-Trimethyl-Histone H3 (Lys27) - 2455635 2455635
      Anti-Trimethyl-Histone H3 (Lys27) - 2194165 2194165
      Anti-Trimethyl-Histone H3 (Lys27) - 2275589 2275589
      Anti-Trimethyl-Histone H3 (Lys27) - 2318778 2318778
      Anti-Trimethyl-Histone H3 (Lys27) - 2506493 2506493
      Anti-trimethyl-Histone H3 (Lys27) - 2120130 2120130
      Anti-trimethyl-Histone H3 (Lys27) - 2137997 2137997
      Anti-trimethyl-Histone H3 (Lys27) - 0607034672 0607034672
      Anti-trimethyl-Histone H3 (Lys27) - 0701050758 0701050758
      Anti-trimethyl-Histone H3 (Lys27) - 1959680 1959680
      Anti-trimethyl-Histone H3 (Lys27) - 1999681 1999681
      Anti-trimethyl-Histone H3 (Lys27) - 2039786 2039786
      Anti-trimethyl-Histone H3 (Lys27) - 2064519 2064519
      Anti-trimethyl-Histone H3 (Lys27) - 2148525 2148525
      Anti-trimethyl-Histone H3 (Lys27) - 2190638 2190638
      Anti-trimethyl-Histone H3 (Lys27) - 24440 24440
      Anti-trimethyl-Histone H3 (Lys27) - 31979 31979
      Anti-trimethyl-Histone H3 (Lys27) - DAM1387952 DAM1387952
      Anti-trimethyl-Histone H3 (Lys27) - DAM1421462 DAM1421462
      Anti-trimethyl-Histone H3 (Lys27) - DAM1514011 DAM1514011
      Anti-trimethyl-Histone H3 (Lys27) - DAM1529471 DAM1529471
      Anti-trimethyl-Histone H3 (Lys27) - DAM1588246 DAM1588246
      Anti-trimethyl-Histone H3 (Lys27) - DAM1612188 DAM1612188
      Anti-trimethyl-Histone H3 (Lys27) - DAM1641103 DAM1641103
      Anti-trimethyl-Histone H3 (Lys27) - DAM1662043 DAM1662043
      Anti-trimethyl-Histone H3 (Lys27) - DAM1662421 DAM1662421
      Anti-trimethyl-Histone H3 (Lys27) - DAM1703508 DAM1703508
      Anti-trimethyl-Histone H3 (Lys27) - DAM1764447 DAM1764447
      Anti-trimethyl-Histone H3 (Lys27) - DAM1823394 DAM1823394
      Anti-trimethyl-Histone H3 (Lys27) - JBC1362834 JBC1362834
      Anti-trimethyl-Histone H3 (Lys27) - JBC1852356 JBC1852356
      Anti-trimethyl-Histone H3 (Lys27) - JBC1854858 JBC1854858
      Anti-trimethyl-Histone H3 (Lys27) - JBC1873477 JBC1873477
      Anti-trimethyl-Histone H3 (Lys27) - JBC1924326 JBC1924326

      References | 44 Available | See All References

      Reference overviewApplicationSpeciesPub Med ID
      XACT, a long noncoding transcript coating the active X chromosome in human pluripotent cells.
      Vallot, Céline, et al.
      Nat. Genet., 45: 239-41 (2013) 2013

      Show Abstract
      23334669
      Loss of WSTF results in spontaneous fluctuations of heterochromatin formation and resolution, combined with substantial changes to gene expression.
      Culver-Cochran, Ashley E and Chadwick, Brian P
      BMC Genomics, 14: 740 (2013) 2013

      Show Abstract
      24168170
      Macro Histone Variants are Critical for the Differentiation of Human Pluripotent Cells.
      Barrero, Maria J, et al.
      J. Biol. Chem., (2013) 2013

      Show Abstract
      Chromatin Immunoprecipitation (ChIP)Human23595991
      Opposing Roles of STAT4 and Dnmt3a in Th1 Gene Regulation.
      Pham, Duy, et al.
      J. Immunol., 191: 902-11 (2013) 2013

      Show Abstract
      23772023
      Hira-Dependent Histone H3.3 Deposition Facilitates PRC2 Recruitment at Developmental Loci in ES Cells.
      Banaszynski, Laura A, et al.
      Cell, 155: 107-20 (2013) 2013

      Show Abstract
      24074864
      Reduced H3K27me3 and DNA Hypomethylation Are Major Drivers of Gene Expression in K27M Mutant Pediatric High-Grade Gliomas.
      Bender, Sebastian, et al.
      Cancer Cell, 24: 660-72 (2013) 2013

      Show Abstract
      24183680
      Hypermethylated in Cancer 1 (HIC1), a Tumor Suppressor Gene Epigenetically Deregulated in Hyperparathyroid Tumors by Histone H3 Lysine Modification.
      Jessica Svedlund,Susanne Koskinen Edblom,Victor E Marquez,Göran Akerström,Peyman Björklund,Gunnar Westin
      The Journal of clinical endocrinology and metabolism 97 2012

      Show Abstract
      22544915
      CRTH2 is a critical regulator of neutrophil migration and resistance to polymicrobial sepsis.
      Makoto Ishii,Koichiro Asano,Ho Namkoong,Sadatomo Tasaka,Kosuke Mizoguchi,Takahiro Asami,Hirofumi Kamata,Yoshifumi Kimizuka,Hiroshi Fujiwara,Yohei Funatsu,Shizuko Kagawa,Jun Miyata,Ken Ishii,Masataka Nakamura,Hiroyuki Hirai,Kinya Nagata,Steven L Kunkel,Naoki Hasegawa,Tomoko Betsuyaku
      Journal of immunology (Baltimore, Md. : 1950) 188 2012

      Show Abstract
      22544936
      The C. elegans H3K27 demethylase UTX-1 is essential for normal development, independent of its enzymatic activity.
      Vandamme, Julien, et al.
      PLoS Genet., 8: e1002647 (2012) 2012

      Show Abstract
      22570628
      Differentiation-independent fluctuation of pluripotency-related transcription factors and other epigenetic markers in embryonic stem cell colonies.
      Gabriela Sustáčková,Soňa Legartová,Stanislav Kozubek,Lenka Stixová,Jiří Pacherník,Eva Bártová
      Stem cells and development 21 2012

      Show Abstract
      21609209

      Brochure

      Title
      Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.
      Product Selection Guide: Shaping Epigenetics Discovery

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