07-352 | Anti-acetyl-Histone H3 (Lys9) Antibody

Beadlyte® Histone-Peptide Specificity Assay
Representative data from a previous lot.
1:1000-1:5000 dilutions were incubated with a cocktail of microspheres conjugated to Histone H3 peptides with the following modifications:

1. acetyl-lysine 9
2. acetyl-
Beadlyte® Histone-Peptide Specificity Assay
Representative data from a previous lot.
100 µL  
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      Replacement Information

      Key Spec Table

      Species Reactivity Key Applications Host Format Antibody Type
      H, Yeast (S. cerevisiae), VrtWB, ChIP, ChIP-seq, MplexRbSerumPolyclonal Antibody
      Catalogue Number 07-352
      Replaces 04-1003
      Brand Family Upstate
      Trade Name
      • Upstate
      Description Anti-acetyl-Histone H3 (Lys9) Antibody
      Alternate Names
      • H3K9Ac
      • H3
      • histone cluster 3
      • H3
      • histone 3
      • H3 histone family, member T
      • Histone H3 (acetyl K9)
      Background Information Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      Product Information
      Format Serum
      • TSA-treated HeLa and NIH/3T3 cells, osteosarcoma tissue.
      Presentation Antiserum containing 0.35% sodium azide and 30% glycerol.
      Application Anti-acetyl-Histone H3 (Lys9) Antibody is a Rabbit Polyclonal Antibody for detection of acetyl-Histone H3 (Lys9) also known as H3K9Ac, Histone H3 (acetyl K9) & has been validated in ChIP, WB, Mplex.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      • ChIP-seq
      • Multiplexing
      Application Notes Chromatin Immunoprecipitation (ChIP):
      An independent laboratory has shown this antibody preferentially immunoprecipitates chromatin from wild type yeast and not from a yeast strain containing a Lysine substitution to Alanine at residue 9.

      Beadlyte ® Histone-Peptide Specificity Assay:
      A 1:1000-1:5000 dilution of this lot was incubated with histone H3 peptides containing various modifications conjugated to Luminex® microspheres. No cross-reactivity with peptides containing acetyl-lysine 14 or acetyl-lysine 27 was observed.
      Biological Information
      Immunogen Ovalbumin-conjugated, synthetic peptide (KQTARAcKSTGGK-C) corresponding to amino acids 4-14 of yeast histone H3 acetylated on lysine 9, with a C-terminal cysteine added for conjugation purposes.
      Epitope a.a. 4-14
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Rabbit
      Specificity Recognizes acetyl-histone H3 (Lys9), Mr 17 kDa. An additional unknown protein was detected at Mr 23 kDa.
      Isotype IgG
      Species Reactivity Human Yeast (S. cerevisiae) Vertebrates
      Species Reactivity Note Yeast and human. Broad species cross-reactivity is expected.
      Antibody Type Polyclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • HIST3H3
      • H3/t
      • H3.4
      • H3/g
      • H3T
      • MGC126888
      • H3t
      • MGC126886
      • H3FT
      • Acetylation
      Purification Method Unpurified
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      TISSUE SPECIFICITY: Expressed in testicular cells.
      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Routinely evaluated by western blot on acid extracts from sodium butyrate treated HeLa cells.

      Western Blot Analysis:
      A 1:5000-1:10,000 dilution of this lot detected acetyl-Histone H3 (Lys9) in acid extracts from sodium butyrate treated HeLa cells (Catalog # 17-305).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Packaging Information
      Material Size 100 µL
      Transport Information
      Supplemental Information




      Certificates of Analysis

      TitleLot Number
      Anti-acetyl-Histone H3 (Lys9)2465196
      Anti-acetyl-Histone H3 (Lys9) - 23889342388934
      Anti-acetyl-Histone H3 (Lys9) - 20901522090152
      Anti-acetyl-Histone H3 (Lys9) - 22031262203126
      Anti-acetyl-Histone H3 (Lys9) - 2239922399
      Anti-acetyl-Histone H3 (Lys9) - 23250912325091
      Anti-acetyl-Histone H3 (Lys9) - 2466124661
      Anti-acetyl-Histone H3 (Lys9) - 2874128741
      Anti-acetyl-Histone H3 (Lys9) - 3138831388
      Anti-acetyl-Histone H3 (Lys9) - DAM1576933DAM1576933
      Anti-acetyl-Histone H3 (Lys9) - DAM1658697DAM1658697
      Anti-acetyl-Histone H3 (Lys9) - DAM1724049DAM1724049
      Anti-acetyl-Histone H3 (Lys9) - DAM1767487DAM1767487
      Anti-acetyl-Histone H3 (Lys9) - DAM1813175DAM1813175
      Anti-acetyl-Histone H3 (Lys9) -25864542586454

      References | 49 Available | See All References

      Reference overviewApplicationSpeciesPub Med ID
      Promoter-associated small double-stranded RNA interacts with heterogeneous nuclear ribonucleoprotein A2/B1 to induce transcriptional activation.
      Jia Hu,Zhong Chen,Ding Xia,Jia Wu,Hua Xu,Zhang-Qun Ye
      The Biochemical journal 447 2012

      Show Abstract
      Increasing phosphatidylinositol (4,5) bisphosphate biosynthesis affects plant nuclear lipids and nuclear functions.
      Catherine B Dieck,Austin Wood,Irena Brglez,Marcela Rojas-Pierce,Wendy F Boss
      Plant physiology and biochemistry : PPB / Société française de physiologie végétale 57 2012

      Show Abstract
      RORγ directly regulates the circadian expression of clock genes and downstream targets in vivo.
      Yukimasa Takeda,Raja Jothi,Veronique Birault,Anton M Jetten
      Nucleic acids research 40 2012

      Show Abstract
      5-azacitidine in aggressive myelodysplastic syndromes regulates chromatin structure at PU.1 gene and cell differentiation capacity.
      N Curik,P Burda,K Vargova,V Pospisil,M Belickova,P Vlckova,F Savvulidi,E Necas,H Hajkova,C Haskovec,J Cermak,M Krivjanska,M Trneny,P Laslo,A Jonasova,T Stopka
      Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K 26 2012

      Show Abstract
      Trichostatin a modified histone covalent pattern and enhanced expression of pluripotent genes in interspecies black-footed cat cloned embryos but did not improve in vitro and in vivo viability.
      Gómez MC, Pope CE, Biancardi MN, Dumas C, Galiguis J, Morris AC, Wang G, Dresser BL
      Cellular reprogramming 13 315-29. doi 2011

      Show Abstract
      Sgf29 binds histone H3K4me23 and is required for SAGA complex recruitment and histone H3 acetylation.
      Bian C, Xu C, Ruan J, Lee KK, Burke TL, Tempel W, Barsyte D, Li J, Wu M, Zhou BO, Fleharty BE, Paulson A, Allali-Hassani A, Zhou JQ, Mer G, Grant PA, Workman JL, Zang J, Min J.
      The EMBO journal 30 2829-42 2011

      Show Abstract
      The transcription factor Pax5 regulates its target genes by recruiting chromatin-modifying proteins in committed B cells.
      McManus, S; Ebert, A; Salvagiotto, G; Medvedovic, J; Sun, Q; Tamir, I; Jaritz, M; Tagoh, H; Busslinger, M
      The EMBO journal 30 2388-404 2011

      Show Abstract
      p53-paralog DNp73 oncogene is repressed by IFNαSTAT2 through the recruitment of the Ezh2 polycomb group transcriptional repressor.
      Testoni B, Schinzari V, Guerrieri F, Gerbal-Chaloin S, Blandino G, Levrero M
      Oncogene 2011

      Show Abstract
      HBO1 is required for H3K14 acetylation and normal transcriptional activity during embryonic development.
      Kueh AJ, Dixon MP, Voss AK, Thomas T
      Mol Cell Biol 31 845-60. Epub 2010 Dec 13. 2011

      Show Abstract Full Text Article
      Carbon monoxide stimulates global protein methylation via its inhibitory action on cystathionine β-synthase.
      Yamamoto T, Takano N, Ishiwata K, Suematsu M
      J Clin Biochem Nutr 48 96-100. Epub 2010 Dec 28. 2011

      Show Abstract Full Text Article


      Histone Modifications