07-158 | Anti-KDR/Flk-1/VEGFR2 Antibody

Immunohistochemistry Analysis:
Representative lot data.
Paraffin-embedded mouse embryo vascular endothelium tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution of Anti-KDR/Flk-1/VEGFR2. Reactivity was detected using the IHC-Select® Detection Kit (Cat. No. DAB050). Staining pattern appears to be restricted endothelial cells with no smooth muscle involvement.
Immunohistochemistry Analysis:
Representative lot data.
Paraffin-embedded mouse embryo v
07-158
200 µL  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MIHC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number 07-158
      Synonyms CD309 Vascular endothelial growth factor receptor 2 VEGFR-2 Fetal liver kinase 1 FLK-1 Kinase insert domain receptor Protein-tyrosine kinase receptor flk-1
      Trade Name
      • Upstate
      Description Anti-KDR/Flk-1/VEGFR2 Antibody
      Background Information The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines. The VEGF receptors stimulate the Ras-MAP kinase pathway, suggesting that it signals as a conventional receptor tyrosine kinase.
      References
      Product Information
      Format Affinity Purified
      Specificity This antibody recognizes KDR/Flk-1/VEGFR2 at the cytoplasmic domain.
      Control
      • PAE cells transfected with CSF/VEGFR2 chimera and treated with CSF
      Presentation Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      Application This KDR/Flk-1/VEGFR2 antibody is validated for use in WB & IHC for the detection of the KDR/Flk-1/VEGFR2 protein.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application Notes Immunohistochemistry Analysis: 1:100 dilution from a representative lot detected KDR/Flk-1/VEGFR2 in mouse embryo vascular endothelium tissue.
      Biological Information
      Immunogen species GST-tagged recombinant protein corresponding to mouse KDR/Flk-1/VEGFR2 at the cytoplasmic domain.
      Epitope Cytoplasmic domain
      Host Rabbit
      Specificity This antibody recognizes KDR/Flk-1/VEGFR2 at the cytoplasmic domain.
      Species Reactivity Mouse
      Species Reactivity Note Demonstrated to react with Mouse. Expected to react with Rat based on a 99% sequence homology and Human based on 92% sequence homology.
      Antibody Type Polyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CD309
      • KDR
      • VEGFR-2
      • VEGFR2
      • FLK1
      • VEGFR
      Purification Method Affinity purified
      UniProt Number
      UniProt Summary FUNCTION: Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

      SIZE: 1356 amino acids; 151527 Da

      SUBUNIT: Interacts with SHB upon VEGF activation. Interacts with HIV-1 Tat.

      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

      SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 7 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Evaluated by Western Blot in PAE cells transfected with CSF/VEGFR2 chimera and treated with CSF.

      Western Blot Analysis: 1:1,000 dilution of this antibody detected KDR/Flk-1/VEGFR2 on 10 µg of PAE cells transfected with CSF/VEGFR2 chimera and treated with CSF.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size 200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-KDR/Flk-1/VEGFR2 - 2152223 2152223
      Anti-KDR/Flk-1/VEGFR2 - 2015412 2015412
      Anti-KDR/Flk-1/VEGFR2 - 20269 20269
      Anti-KDR/Flk-1/VEGFR2 - 2250517 2250517
      Anti-KDR/Flk-1/VEGFR2 - 2318093 2318093
      Anti-KDR/Flk-1/VEGFR2 - 24889 24889
      Anti-KDR/Flk-1/VEGFR2 - 29705 29705
      Anti-KDR/Flk-1/VEGFR2 - 31459 31459
      Anti-KDR/Flk-1/VEGFR2 - NG1901588 NG1901588

      References

      Reference overviewPub Med ID
      Calsperin is a testis-specific chaperone required for sperm fertility.
      Ikawa M, Tokuhiro K, Yamaguchi R, Benham AM, Tamura T, Wada I, Satouh Y, Inoue N, Okabe M
      J Biol Chem 286 5639-46. Epub 2010 Dec 3. 2011

      Show Abstract Full Text Article
      21131354
      Compartmentalizing VEGF-induced ERK2/1 signaling in placental artery endothelial cell caveolae: a paradoxical role of caveolin-1 in placental angiogenesis in vitro.
      Wu-Xiang Liao, Lin Feng, Honghai Zhang, Jing Zheng, Thomas R Moore, Dong-Bao Chen, Wu-Xiang Liao, Lin Feng, Honghai Zhang, Jing Zheng, Thomas R Moore, Dong-Bao Chen
      Molecular endocrinology (Baltimore, Md.) 23 1428-44 2009

      Show Abstract Full Text Article
      19477952
      Receptor chimeras indicate that the vascular endothelial growth factor receptor-1 (VEGFR-1) modulates mitogenic activity of VEGFR-2 in endothelial cells.
      Rahimi, N, et al.
      J. Biol. Chem., 275: 16986-92 (2000) 2000

      Show Abstract
      10747927
      Vascular endothelial growth factor and hepatocyte growth factor levels are differentially elevated in patients with advanced retinopathy of prematurity.
      Lashkari, K, et al.
      Am. J. Pathol., 156: 1337-44 (2000) 2000

      Show Abstract
      10751359
      A role for cadherin-5 in regulation of vascular endothelial growth factor receptor 2 activity in endothelial cells.
      Rahimi, N and Kazlauskas, A
      Mol. Biol. Cell, 10: 3401-7 (1999) 1999

      Show Abstract
      10512875