07-122 | Anti-Kalirin Antibody

WB: Rat brain microsomal protein preparation was probed with anti-Kalirin (2μg/ml). Arrow indicates Kalirin (190kDa).
WB: Rat brain microsomal protein preparation was probed with anti-Kalirin (2μg/ml). Arrow indicates
07-122
200 µg  
Retrieving price...
Price could not be retrieved
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited Availability Limited Availability
Discontinued
There is a newer version of this product
Limited Quantities Available
Availability to be confirmed
      Will advise

      Special Offers

       

      Contact Customer Service

         CompareClick To Print This Page

      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, RIP, ICC, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number 07-122
      Brand Family Upstate
      Trade Name
      • Upstate
      Description Anti-Kalirin Antibody
      References
      Product Information
      Format Purified
      Specificity Kalirin; does not cross react with Trio
      Presentation 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide prior to the addition of glycerol to 30%
      Applications
      Application Anti-Kalirin Antibody is an antibody against Kalirin for use in IP, WB & IC.
      Key Applications
      • Immunoprecipitation
      • Immunocytochemistry
      • Western Blotting
      Biological Information
      Immunogen species GST fusion protein corresponding to residues 517-976 of rat Kalirin, cleaved with thrombin to remove the GST moiety; referred to as JH2582
      Host Rabbit
      Specificity Kalirin; does not cross react with Trio
      Isotype IgG
      Species Reactivity Mouse Rat
      Antibody Type Polyclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein that interacts with the huntingtin-associated protein 1, which is a huntingtin binding protein that may function in vesicle trafficking. Alternatively spliced transcript variants encoding different isoforms have been described.
      Gene Symbol
      • KALRN
      • HAPIP
      • DUO
      • FLJ16443
      • Kalirin
      • duo
      • DUET
      • Hs.8004
      • TRAD
      Purification Method Protein A chromatography
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: O60229 # Promotes the exchange of GDP by GTP. Activates specific Rho GTPase family members, thereby inducing various signaling mechanisms that regulate neuronal shape, growth, and plasticity, through their effects on the actin cytoskeleton. Induces lamellipodia independent of its GEF activity.
      COFACTOR: Magnesium.
      SIZE: 2985 amino acids; 340174 Da
      SUBUNIT: Interacts with the C-terminal of peptidylglycine alpha- amidating monooxygenase (PAM) and with the huntingtin-associated protein 1 (HAP1) (By similarity).
      SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, cytoskeleton. Note=Associated with the cytoskeleton.
      TISSUE SPECIFICITY: Isoform 2 is brain specific. Highly expressed in cerebral cortex, putamen, amygdala, hippocampus and caudate nucleus. Weakly expressed in brain stem and cerebellum. Isoform 4 is expressed in skeletal muscle.
      DOMAIN: SwissProt: O60229 The two GEF domains catalyze nucleotide exchange for RAC1 and RhoA which are bound by DH1 and DH2 respectively. The two GEF domains appear to play differing roles in neuronal development and axonal outgrowth. SH3 1 binds to the first GEF domain inhibiting GEF activity only when in the presence of a PXXP peptide, suggesting that the SH3 domain/peptide interaction mediates binding to GEF1. CRK1 SH3 domain binds to and inhibits GEF1 activity (By similarity).
      PTM: Autophosphorylated.
      DISEASE: SwissProt: O60229 # Genetic variation in KALRN is associated with susceptibility to coronary heart disease type 5 (CHDS5) [MIM:608901]. CHD is the leading cause of death and disability worldwide. CHD is multifactorial disease with a strong genetic component. Classic epidemiologic studies have revealed many risk factors for CHD, including age, sex, hypertension, dyslipidemia, diabetes mellitus, smoking, and physical inactivity.
      SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 1 CRAL-TRIO domain. & Contains 2 DH (DBL-homology) domains. & Contains 1 fibronectin type-III domain. & Contains 1 Ig-like C2-type (immunoglobulin-like) domain. & Contains 2 PH domains. & Contains 1 protein kinase domain. & Contains 1 RPR domain. & Contains 2 SH3 domains. & Contains 5 spectrin repeats.
      MISCELLANEOUS: Called DUO because the encoded protein is closely related to but shorter than TRIO.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance routinely evaluated by immunoblot on RIPA lysates from rat brain microsomal protein preparation; long exposure times may be required
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions 2 years at -20°C
      Packaging Information
      Material Size 200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Languages
      Deutschland

      Certificates of Analysis

      TitleLot Number
      07-122 - 2280413 2280413
      Anti-Kalirin (rabbit polyclonal IgG) - 2015760 2015760
      Anti-Kalirin (rabbit polyclonal IgG) - 2032852 2032852
      Anti-Kalirin (rabbit polyclonal IgG) - 2155411 2155411
      Anti-Kalirin (rabbit polyclonal IgG) - 2332542 2332542
      Anti-Kalirin - 20095 20095
      Anti-Kalirin - 2043526 2043526
      Anti-Kalirin - DAM1576930 DAM1576930
      Anti-Kalirin - DAM1606754 DAM1606754
      Anti-Kalirin - DAM1647874 DAM1647874
      Anti-Kalirin - DAM1690659 DAM1690659
      Anti-Kalirin - NG1844879 NG1844879

      References

      Reference overviewPub Med ID
      Social, Communication, and Cortical Structural Impairments in Epac2-Deficient Mice.
      Srivastava, Deepak P, et al.
      J. Neurosci., 32: 11864-11878 (2012) 2012

      Show Abstract
      22915127
      β-Amyloid 42/40 ratio and kalirin expression in Alzheimer disease with psychosis.
      Patrick S Murray,Caitlin M Kirkwood,Megan C Gray,Milos D Ikonomovic,William R Paljug,Eric E Abrahamson,Ruth A Henteleff,Ronald L Hamilton,Julia K Kofler,William E Klunk,Oscar L Lopez,Peter Penzes,Robert A Sweet
      Neurobiology of aging 33 2012

      Show Abstract
      22429885
      An isoform of kalirin, a brain-specific GDP/GTP exchange factor, is enriched in the postsynaptic density fraction.
      Penzes, P, et al.
      J. Biol. Chem., 275: 6395-403 (2000) 2000

      Show Abstract
      10692441
      Kalirin inhibition of inducible nitric-oxide synthase.
      Ratovitski, E A, et al.
      J. Biol. Chem., 274: 993-9 (1999) 1999

      Show Abstract
      9873042
      Kalirin, a multifunctional PAM COOH-terminal domain interactor protein, affects cytoskeletal organization and ACTH secretion from AtT-20 cells.
      Mains, R E, et al.
      J. Biol. Chem., 274: 2929-37 (1999) 1999

      Show Abstract
      9915831