07-001 | Anti-p47-phox Antibody

WB: HL-60 cell lysate was probed with anti-p47-phox (1:2000 dilution). Arrow indicates p47-phox.
07-001
200 μL  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, WB, IPRbSerumPolyclonal Antibody
      Description
      Catalogue Number 07-001
      Brand Family Upstate
      Trade Name
      • Upstate
      Description Anti-p47-phox Antibody
      References
      Product Information
      Format Serum
      Specificity Recognizes p47 phox.
      Control
      • HL-60 cell lysate
      Presentation Immunoaffinity Purified immunoglobulin in 0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide, 5mg/ml BSA before the addition of glycerol to 30%
      Applications
      Application Anti-p47-phox Antibody detects level of p47-phox & has been published & validated for use in IC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      • Immunoprecipitation
      Biological Information
      Immunogen species GST fusion protein corresponding to full-length human p47-phox
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Rabbit
      Specificity Recognizes p47 phox.
      Isotype IgG
      Species Reactivity Human
      Antibody Type Polyclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease.
      Gene Symbol
      • NCF1
      • NCF-1
      • p47phox
      • NCF1A
      • SH3PXD1A
      • p47-phox
      • NOXO2
      • NCF-47K
      Purification Method Unpurified
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: P14598 # NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
      SIZE: 390 amino acids; 44683 Da
      SUBCELLULAR LOCATION: Cytoplasm.
      DISEASE:SwissProt: P14598 # Defects in NCF1 are the cause of autosomal cytochrome-b- positive chronic granulomatous disease type 1 (CGD) [MIM:233700]; an autosomal recessive form.
      SIMILARITY: SwissProt: P14598 ## Contains 1 PX (phox homology) domain. & Contains 2 SH3 domains.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance routinely evaluated by immunoblot on RIPA lysates from HL-60 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size 200 μL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-p47-phox (rabbit antiserum) - 2188668 2188668
      Anti-p47-phox (rabbit antiserum) - 2266825 2266825
      Anti-p47-phox (rabbit antiserum) - 2328349 2328349
      Anti-p47-phox (rabbit antiserum) - 2366551 2366551
      Anti-p47-phox (rabbit antiserum) -2491183 2491183
      Anti-p47-phox - 19136 19136
      Anti-p47-phox - 21048 21048
      Anti-p47-phox - 26347 26347
      Anti-p47-phox - 29746 29746
      Anti-p47-phox - DAM1394795 DAM1394795
      Anti-p47-phox - DAM1413939 DAM1413939
      Anti-p47-phox - DAM1754397 DAM1754397
      Anti-p47-phox - DAM1792490 DAM1792490
      Anti-p47-phox - DAM1811082 DAM1811082
      Anti-p47-phox - JBC1361869 JBC1361869
      Anti-p47-phox - JBC1908439 JBC1908439

      References

      Reference overviewApplicationPub Med ID
      Antrocamphin A, an anti-inflammatory principal from the fruiting body of Taiwanofungus camphoratus , and its mechanisms.
      Hsieh YH, Chu FH, Wang YS, Chien SC, Chang ST, Shaw JF, Chen CY, Hsiao WW, Kuo YH, Wang SY
      J Agric Food Chem 58 3153-8. 2010

      Show Abstract
      20128588
      Molecular diagnosis of X-linked chronic granulomatous disease in Iran.
      S Teimourian, Z Rezvani, M Badalzadeh, C Kannengiesser, D Mansouri, M Movahedi, E Zomorodian, N Parvaneh, S Mamishi, Z Pourpak, M Moin, S Teimourian, Z Rezvani, M Badalzadeh, C Kannengiesser, D Mansouri, M Movahedi, E Zomorodian, N Parvaneh, S Mamishi, Z Pourpak, M Moin
      International journal of hematology 87 398-404 2008

      Show Abstract
      18322777
      A myocardial Nox2 containing NAD(P)H oxidase contributes to oxidative stress in human atrial fibrillation.
      Young M Kim, Tomasz J Guzik, Yin Hua Zhang, Mei Hua Zhang, Hassan Kattach, Chandi Ratnatunga, Ravi Pillai, Keith M Channon, Barbara Casadei
      Circulation research 97 629-36 2005

      Show Abstract
      16123335
      Molecular characterization of a superoxide-generating NAD(P)H oxidase in the ventilatory muscles
      Javesghani, D., et al
      Am J Respir Crit Care Med, 165:412-8 (2002) 2002

      Immunoblotting (Western), Immunohistochemistry (Tissue)11818330
      Localization of a constitutively active, phagocyte-like NADPH oxidase in rabbit aortic adventitia: enhancement by angiotensin II
      Pagano, P J, et al
      Proc Natl Acad Sci USA, 94:14483-8 (1997) 1997

      9405639