07-001 | Anti-p47-phox Antibody

WB: HL-60 cell lysate was probed with anti-p47-phox (1:2000 dilution). Arrow indicates p47-phox.
200 µL  
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      Replacement Information

      Key Spec Table

      Species Reactivity Key Applications Host Format Antibody Type
      HWB, ICC, IPRbSerumPolyclonal Antibody
      Catalogue Number 07-001
      Brand Family Upstate
      Trade Name
      • Upstate
      Description Anti-p47-phox Antibody
      Product Information
      Format Serum
      • HL-60 cell lysate
      Presentation Immunoaffinity Purified immunoglobulin in 0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide, 5mg/ml BSA before the addition of glycerol to 30%
      Application Anti-p47-phox Antibody detects level of p47-phox & has been published & validated for use in IC, IP & WB.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      Biological Information
      Immunogen GST fusion protein corresponding to full-length human p47-phox
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Rabbit
      Specificity Recognizes p47 phox.
      Isotype IgG
      Species Reactivity Human
      Antibody Type Polyclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease.
      Gene Symbol
      • NCF-1
      • NCF1
      • NCF-47K
      • NOXO2
      • p47-phox
      • SH3PXD1A
      • NCF1A
      • p47phox
      Purification Method Unpurified
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: P14598 # NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
      SIZE: 390 amino acids; 44683 Da
      DISEASE:SwissProt: P14598 # Defects in NCF1 are the cause of autosomal cytochrome-b- positive chronic granulomatous disease type 1 (CGD) [MIM:233700]; an autosomal recessive form.
      SIMILARITY: SwissProt: P14598 ## Contains 1 PX (phox homology) domain. & Contains 2 SH3 domains.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance routinely evaluated by immunoblot on RIPA lysates from HL-60 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size 200 µL
      Transport Information
      Supplemental Information


      Certificates of Analysis

      TitleLot Number
      Anti-p47-phox (rabbit antiserum) - 21886682188668
      Anti-p47-phox (rabbit antiserum) - 22668252266825
      Anti-p47-phox (rabbit antiserum) - 23283492328349
      Anti-p47-phox (rabbit antiserum) - 23665512366551
      Anti-p47-phox (rabbit antiserum) -24911832491183
      Anti-p47-phox - 1913619136
      Anti-p47-phox - 2104821048
      Anti-p47-phox - 2634726347
      Anti-p47-phox - 2974629746
      Anti-p47-phox - DAM1394795DAM1394795
      Anti-p47-phox - DAM1413939DAM1413939
      Anti-p47-phox - DAM1754397DAM1754397
      Anti-p47-phox - DAM1792490DAM1792490
      Anti-p47-phox - DAM1811082DAM1811082
      Anti-p47-phox - JBC1361869JBC1361869
      Anti-p47-phox - JBC1908439JBC1908439


      Reference overviewApplicationPub Med ID
      Antrocamphin A, an anti-inflammatory principal from the fruiting body of Taiwanofungus camphoratus , and its mechanisms.
      Hsieh YH, Chu FH, Wang YS, Chien SC, Chang ST, Shaw JF, Chen CY, Hsiao WW, Kuo YH, Wang SY
      J Agric Food Chem 58 3153-8. 2010

      Show Abstract
      Molecular diagnosis of X-linked chronic granulomatous disease in Iran.
      S Teimourian, Z Rezvani, M Badalzadeh, C Kannengiesser, D Mansouri, M Movahedi, E Zomorodian, N Parvaneh, S Mamishi, Z Pourpak, M Moin, S Teimourian, Z Rezvani, M Badalzadeh, C Kannengiesser, D Mansouri, M Movahedi, E Zomorodian, N Parvaneh, S Mamishi, Z Pourpak, M Moin
      International journal of hematology 87 398-404 2008

      Show Abstract
      A myocardial Nox2 containing NAD(P)H oxidase contributes to oxidative stress in human atrial fibrillation.
      Young M Kim, Tomasz J Guzik, Yin Hua Zhang, Mei Hua Zhang, Hassan Kattach, Chandi Ratnatunga, Ravi Pillai, Keith M Channon, Barbara Casadei
      Circulation research 97 629-36 2005

      Show Abstract
      Molecular characterization of a superoxide-generating NAD(P)H oxidase in the ventilatory muscles
      Javesghani, D., et al
      Am J Respir Crit Care Med, 165:412-8 (2002) 2002

      Immunoblotting (Western), Immunohistochemistry (Tissue)11818330
      Localization of a constitutively active, phagocyte-like NADPH oxidase in rabbit aortic adventitia: enhancement by angiotensin II
      Pagano, P J, et al
      Proc Natl Acad Sci USA, 94:14483-8 (1997) 1997