06-942 | Anti-acetyl-Histone H3 (Lys9) Antibody

Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from HeLa cells (1 X 10e5 cell equivalents per IP) were subjected to chromatin immunoprecipitation with the Magna ChIP™ HiSense Kit (Cat. # 17-10460), using 5 μg of either a negative control rabbit IgG or Cat. No 06-942, Anti-Acetyl-Histone H3 (Lys9) antibody. Successful immunoprecipitation of acetyl-Histone H3 (Lys9)-associated DNA fragments was verified by qPCR using ChIP Primers GAPDH promoter (Part No. 22-004) as a positive locus, and β-Globin primers as a negative locus.
Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from
06-942
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species Reactivity Key Applications Host Format Antibody Type
      R, H, MChIP-seq, WB, ChIP, DB, FCRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number 06-942
      Replaces 04-1003
      Brand Family Upstate
      Synonyms histone cluster 3, H3 H3K9Ac Histone H3 (acetyl K9) H3 histone family, member T histone 3, H3
      Trade Name
      • Upstate
      Description Anti-acetyl-Histone H3 (Lys9) Antibody
      Background Information Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      References
      Product Information
      Format Purified
      Presentation Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide with 30% glycerol.
      Applications
      Application Anti-acetyl-Histone H3 (Lys9) Antibody is a Rabbit Polyclonal Antibody for detection of Histone H3 acetylated on lysine 9. This purified Ab, also known as Anti-H3K9Ac, is specificity verified by dot blot (DB), published in peer reviewed journals, and validated in WB, ChIP, ChIP-seq, FC.
      Key Applications
      • ChIP-seq
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      • Dot Blot
      • Flow Cytometry
      Application Notes Chromatin Immunoprecipitation/ChIP-seq Analysis: A representative lot immunoprecipitated acetyl-Histone H3 (Lys9) in 5 µg of HeLa cell lysate.
      Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected acetyl-Histone H3 (Lys9) in an Absurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance™ Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
      Flow Cytometry Analysis: 0.25 µg of this antibody from a representative lot detected acetyl-Histone H3 (Lys9) in 1X10E6 HEK293 cells.
      Biological Information
      Immunogen KLH-conjugated synthetic peptide (ARTKQTARK(Ac)STG-C) corresponding to amino acids 1-12 of Histone H3 with acetylated Lys9.
      Epitope Surrounding H3K9ac
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Rabbit
      Specificity Histone H3 acetylated at position 9.
      Isotype IgG
      Species Reactivity Rat Human Mouse
      Species Reactivity Note Human, Mouse, Rat, although this peptide sequence is identical in a wide range of animal and plant species.
      Antibody Type Polyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MGC126886
      • HIST1H3A
      • H3FA
      • HIST1H3B
      • H3FL
      • HIST1H3C
      • H3FC
      • HIST1H3D
      • H3FB
      • HIST1H3E
      • H3FD
      • HIST1H3F
      • H3FI
      • HIST1H3G
      • H3FH
      • HIST1H3H
      • H3FK
      • HIST1H3I
      • H3FF
      • HIST1H3J
      • H3FJ
      • HIST3H3
      • H3FT
      • H3T
      Modifications
      • Acetylation
      Purification Method Protein A purfied
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Evaluated by Western Blotting in sodium butyrate treated HeLa cell lysate.

      Western Blotting Analysis: A 1:10,000 dilution of this antibody detected acetyl-Histone H3 (Lys9) in 10 µg of sodium butyrate treated HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size 200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Languages
      Deutschland

      Certificates of Analysis

      TitleLot Number
      Anti-acetyl-Histone H3 (Lys9) - 21351722135172
      Anti-acetyl-Histone H3 (Lys9) - 1874018740
      Anti-acetyl-Histone H3 (Lys9) - 19734141973414
      Anti-acetyl-Histone H3 (Lys9) - 21112832111283
      Anti-acetyl-Histone H3 (Lys9) - 21784192178419
      Anti-acetyl-Histone H3 (Lys9) - 2189721897
      Anti-acetyl-Histone H3 (Lys9) - 21931542193154
      Anti-acetyl-Histone H3 (Lys9) - 22795102279510
      Anti-acetyl-Histone H3 (Lys9) - 2399723997
      Anti-acetyl-Histone H3 (Lys9) - 25110872511087
      Anti-acetyl-Histone H3 (Lys9) - 25621002562100
      Anti-acetyl-Histone H3 (Lys9) - 3163631636
      Anti-acetyl-Histone H3 (Lys9) -25246852524685
      Anti-acetyl-Histone H3 (Lys9) -25493942549394
      Anti-acetyl-Histone H3 (Lys9), - 23878122387812
      Anti-acetyl-Histone H3 (Lys9), - 24287142428714

      References | 29 Available | See All References

      Reference overviewApplicationPub Med ID
      Association of the interferon-β gene with pericentromeric heterochromatin is dynamically regulated during virus infection through a YY1-dependent mechanism.
      T Josse,H Mokrani-Benhelli,R Benferhat,E Shestakova,Z Mansuroglu,H Kakanakou,A Billecocq,M Bouloy,Eliette Bonnefoy
      Nucleic acids research 40 2012

      Show Abstract
      22287632
      An essential role for a mammalian SWI/SNF chromatin-remodeling complex during male meiosis.
      Yuna Kim,Andrew M Fedoriw,Terry Magnuson
      Development (Cambridge, England) 139 2012

      Show Abstract
      22318225
      Epigenetic reprogramming as a key contributor to melanocyte malignant transformation.
      Molognoni F, Cruz AT, Meliso FM, Morais AS, Souza CF, Xander P, Bischof JM, Costa FF, Soares MB, Liang G, Jones PA, Jasiulionis MG.
      Epigenetics : official journal of the DNA Methylation Society 6 450-64 2011

      Show Abstract
      21343701
      SIRT6 in mouse spermatogenesis is modulated by diet-induced obesity.
      Palmer NO, Fullston T, Mitchell M, Setchell BP, Lane M.
      Reproduction, fertility, and development 23 929-39 2011

      Show Abstract
      21871212
      Post-transcription initiation function of the ubiquitous SAGA complex in tissue-specific gene activation.
      Weake, Vikki M, et al.
      Genes Dev., 25: 1499-509 (2011) 2011

      Show Abstract
      21764853
      Mouse Rankl expression is regulated in T cells by c-Fos through a cluster of distal regulatory enhancers designated the T cell control region.
      Bishop KA, Coy HM, Nerenz RD, Meyer MB, Pike JW
      The Journal of biological chemistry 2011

      Show Abstract
      21487009
      Chromatin states of core pluripotency-associated genes in pluripotent, multipotent and differentiated cells.
      S Barrand, P Collas
      Biochemical and biophysical research communications 391 762-7 2010

      Show Abstract
      19944068
      Chromatin environment of histone variant H3.3 revealed by quantitative imaging and genome-scale chromatin and DNA immunoprecipitation.
      Delbarre E, Jacobsen BM, Reiner AH, Sørensen AL, Küntziger T, Collas P
      Mol Biol Cell 21 1872-84. Epub 2010 Apr 7. 2010

      Show Abstract Full Text Article
      20375147
      Characterization of an antagonistic switch between histone H3 lysine 27 methylation and acetylation in the transcriptional regulation of Polycomb group target genes.
      Pasini D, Malatesta M, Jung HR, Walfridsson J, Willer A, Olsson L, Skotte J, Wutz A, Porse B, Jensen ON, Helin K
      Nucleic Acids Res 2010

      Show Abstract
      20385584
      MDM2 recruitment of lysine methyltransferases regulates p53 transcriptional output.
      Chen L, Li Z, Zwolinska AK, Smith MA, Cross B, Koomen J, Yuan ZM, Jenuwein T, Marine JC, Wright KL, Chen J
      EMBO J 2010

      Show Abstract
      20588255

      Technical Info

      Title
      Anti-acetyl-Histone H3 (Lys9) ChIP-Seq Analysis