06-896 | Anti-GFP (Green Fluorescent Protein) Antibody

WB: rat brain lysate containing 25ng of GFP was probed with 1μg/ml of anti-GFP
06-896
200  μg
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      VrtWB, ICCChPurifiedPolyclonal Antibody
      Description
      Catalogue Number 06-896
      Brand Family Upstate
      Synonyms Green fluorescent protein
      Trade Name
      • Upstate
      Description Anti-GFP (Green Fluorescent Protein) Antibody
      Background Information Green fluorescent protein (GFP) is a 29 kDa fluorescent protein isolated from the jellyfish Aequorea victoria. Upon exposure to blue light (488 nm), GFP strongly fluoresces (Emission max: 507 nm). GFP can be fused to proteins without significantly interfering with their assembly and function, making GFP a powerful tool for monitoring gene expression and protein localization in living cells.
      References
      Product Information
      Format Purified
      Specificity Recognizes green fluorescent protein (GFP), Mr 30 kDa, and GFP-fusion proteins.
      Control
      • Rat brain lysate, cells expressing GFP protein.
      Presentation Purified chicken polyclonal IgY from egg yolk in buffer containing 70% storage buffer (0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide) and 30% glycerol. Store at -20°C.
      Applications
      Application Anti-GFP (Green Fluorescent Protein) Antibody detects level of GFP (Green Fluorescent Protein) & has been published & validated for use in IC & WB.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application Notes Immunocytochemistry:
      This antibody was reported to show positive immunostaining in cells transfected with an expression vector encoding Green Fluorescent Protein.
      Biological Information
      Immunogen species His-tagged green fluorescent protein of Aequorea victoria.
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Chicken
      Specificity Recognizes green fluorescent protein (GFP), Mr 30 kDa, and GFP-fusion proteins.
      Isotype IgY
      Species Reactivity Vertebrates
      Species Reactivity Note Vertebrates
      Antibody Type Polyclonal Antibody
      Gene Symbol
      • GFP
      Purification Method Ammonium Sulfate Precipitation and PEG
      UniProt Number
      UniProt Summary FUNCTION: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
      BIOPHYSICOCHEMICAL PROPERTIES:
      Absorption: Abs(max)=395 nm;
      Note=Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm;
      SUBUNIT: Monomer.
      TISSUE SPECIFICITY: Photocytes.
      PTM: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
      BIOTECHNOLOGY: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
      BIOTECHNOLOGY: Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.
      SIMILARITY: Belongs to the GFP family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Routinely evaluated by Western Blot on rat brain lysate containing purified GFP (Catalog #14-392) or a GFP-fusion protein from a transfected E. coli cell lysate.

      Western Blot Analysis:
      0.5-2 μg/mL of this lot detected Green Fluorescent Protein from 50 μg of rat brain lysate containing 25 ng of purified Green Fluorescent Protein (Catalog # 14-392). A previous lot of this antibody also detected a GFP-fusion protein from a transfected E. coli cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
      Packaging Information
      Qty/Pck 200 µg
      Material Size 200
      Material Package μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-GFP (Green Fluorescent Protein) - 2382624 2382624
      Anti-GFP (Green Fluorescent Protein) - 2453237 2453237
      Millipore - COA - Anti-GFP (Green Fluorescent Protein) (chicken polyclonal IgY) - DAM1430003 DAM1430003
      Millipore - COA - Anti-GFP (Green Fluorescent Protein) - 1964543 1964543
      Millipore - COA - Anti-GFP (Green Fluorescent Protein) - 2028729 2028729
      Millipore - COA - Anti-GFP (Green Fluorescent Protein) - 2197506 2197506
      Millipore - COA - Anti-GFP (Green Fluorescent Protein) - DAM1543699 DAM1543699
      Millipore - COA - Anti-GFP (Green Fluorescent Protein) - DAM1636098 DAM1636098
      Millipore - COA - Anti-GFP (Green Fluorescent Protein) - DAM1713200 DAM1713200
      Millipore - COA - Anti-GFP (Green Fluorescent Protein) - DAM1767484 DAM1767484
      Millipore - COA - Anti-GFP (Green Fluorescent Protein) - DAM1795377 DAM1795377
      Millipore - COA - Anti-GFP (Green Fluorescent Protein) - JBC1858399 JBC1858399
      Millipore - COA - Anti-GFP (Green Fluorescent Protein) - JBC1927869 JBC1927869
      Millipore - COAs - Anti-GFP (Green Fluorescent Protein) - 17901 17901
      Millipore - COAs - Anti-GFP (Green Fluorescent Protein) - 20505 20505
      Millipore - COAs - Anti-GFP (Green Fluorescent Protein) - 22694 22694
      Millipore - COAs - Anti-GFP (Green Fluorescent Protein) - 29732 29732

      References

      Reference overviewPub Med ID
      MARK2/Par-1 guides the directionality of neuroblasts migrating to the olfactory bulb.
      Sheyla Mejia-Gervacio,Kerren Murray,Tamar Sapir,Richard Belvindrah,Orly Reiner,Pierre-Marie Lledo
      Molecular and cellular neurosciences 49 2012

      Show Abstract
      22061967
      Molecular and electrophysiological characterization of GFP-expressing CA1 interneurons in GAD65-GFP mice.
      Corette J Wierenga,Fiona E Müllner,Ilka Rinke,Tara Keck,Valentin Stein,Tobias Bonhoeffer
      PloS one 5 2010

      Show Abstract Full Text Article
      21209836
      Green fluorescent protein: applications in cell biology.
      Gerdes, H H and Kaether, C
      FEBS Lett., 389: 44-7 (1996) 1996

      Show Abstract
      8682203
      Green fluorescent protein as a reporter of gene expression and protein localization.
      Kain, S R, et al.
      BioTechniques, 19: 650-5 (1995) 1995

      Show Abstract
      8777060