06-866 | Anti-acetyl-Histone H4 Antibody

Sonicated chromatin prepared from untreated HeLa cells (1 X 106 cell equivalents) was subjected to chromatin immunoprecipitation using 4 µL of either a normal rabbit antiserum or Anti-Acetyl Histone H4 serum and the Magna ChIP A (Cat. # 06-866) Kit. Successful immunoprecipitation of acetyl histone H4 associated DNA fragments was verified by qPCR using control ChIP Primers flanking the human GAPDH promoter.
Sonicated chromatin prepared from untreated HeLa cells (1 X 106 cell equivalents) was subjected to c
06-866
200  µL
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Eu, H, TChIP, WB, ICCRbSerumPolyclonal Antibody
      Description
      Catalogue Number 06-866
      Brand Family Upstate
      Synonyms histone cluster 1, H4a H4 histone family, member A histone 1, H4a
      Trade Name
      • Upstate
      Description Anti-acetyl-Histone H4 Antibody
      Background Information Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
      References
      Product Information
      Format Serum
      Specificity Acetylated histone H4, acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells, may cross-react with other acetylated proteins.
      Control
      • TSA-treated Jurkat cells. For a negative control, perform a no-antibody immunoprecipitation by incubating the supernatant fraction with 60μl of Salmon Sperm DNA/Protein A Agarose- 50% Slurry. Transcriptionally unactivated DNA samples should be prepared as controls for PCR.
      Presentation Whole antiserum containing 0.05% sodium azide.
      Applications
      Application Use Anti-acetyl-Histone H4 Antibody (Rabbit Polyclonal Antibody) validated in ChIP, WB, ICC to detect acetyl-Histone H4 also known as H4 histone family member A, histone 1-H4a.
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • Western Blotting
      • Immunocytochemistry
      Application Notes Chromatin Immunoprecipitation:
      5-10 μL of this lot immunoprecipitated transcriptionally active chromatin containing acetylated histone H4 from 2 X 106 serum stimulated HeLa cells.
      Biological Information
      Immunogen species KLH-conjugated peptide corresponding to a.a. 2-19 of Tetrahymena histone H4 [AGGAcKGGAcKGMGAcKVGAAcKRHS-C], acetylated on lysines 5, 8, 12 and 16.
      Epitope a.a. 2-19
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Rabbit
      Specificity Acetylated histone H4, acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells, may cross-react with other acetylated proteins.
      Isotype IgG
      Species Reactivity Eukaryote Human Tetrahymena
      Species Reactivity Note Human and Tetrahymena. Other species not tested, but expected to cross-react since Histone H4 is well conserved.
      Antibody Type Polyclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
      Gene Symbol
      • H4/E
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • IST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • 4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      Modifications
      • Acetylation
      Purification Method Unpurified
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 103 amino acids; 11367 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
      SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Routinely evaluated by western blot on Tetrahymena macronuclei or acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate.

      Western Blot Analysis:
      1:2000 dilution of this lot detected acetylated histone H4 in acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at -20°C from date of receipt.
      Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Qty/Pck 200 µL
      Material Size 200
      Material Package µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-acetyl-Histone H4 - 2384725 2384725
      Anti-acetyl-Histone H4 - 2459612 2459612
      Anti-acetyl-Histone H4 - 2282163 2282163
      Millipore - COA - Anti-acetyl-Histone H4 (rabbit antiserum) - DAM1427455 DAM1427455
      Millipore - COA - Anti-acetyl-Histone H4 - 2019734 2019734
      Millipore - COA - Anti-acetyl-Histone H4 - 2073114 2073114
      Millipore - COA - Anti-acetyl-Histone H4 - 2153152 2153152
      Millipore - COA - Anti-acetyl-Histone H4 - DAM1416550 DAM1416550
      Millipore - COA - Anti-acetyl-Histone H4 - DAM1655258 DAM1655258
      Millipore - COA - Anti-acetyl-Histone H4 - DAM1690655 DAM1690655
      Millipore - COA - Anti-acetyl-Histone H4 - DAM1713199 DAM1713199
      Millipore - COA - Anti-acetyl-Histone H4 - DAM1739166 DAM1739166
      Millipore - COA - Anti-acetyl-Histone H4 - DAM1770301 DAM1770301
      Millipore - COA - Anti-acetyl-Histone H4 - DAM1834939 DAM1834939
      Millipore - COA - Anti-acetyl-Histone H4 - JBC1873473 JBC1873473
      Millipore - COA - Anti-acetyl-Histone H4 - JBC1885060 JBC1885060
      Millipore - COAs - Anti-acetyl-Histone H4 - 17428 17428
      Millipore - COAs - Anti-acetyl-Histone H4 - 18784 18784
      Millipore - COAs - Anti-acetyl-Histone H4 - 19336 19336
      Millipore - COAs - Anti-acetyl-Histone H4 - 20667 20667
      Millipore - COAs - Anti-acetyl-Histone H4 - 23201 23201
      Millipore - COAs - Anti-acetyl-Histone H4 - 26393 26393
      Millipore - COAs - Anti-acetyl-Histone H4 - 29532 29532
      Millipore - COAs - Anti-acetyl-Histone H4 - 31992 31992

      References | 47 Available | See All References

      Reference overviewApplicationSpeciesPub Med ID
      Acetylation limits 53BP1 association with damaged chromatin to promote homologous recombination.
      Tang, Jiangbo, et al.
      Nat. Struct. Mol. Biol., 20: 317-25 (2013) 2013

      Show Abstract
      23377543
      The CpG island shore of the GLT-1 gene acts as a methylation-sensitive enhancer.
      Tatjana Perisic,Florian Holsboer,Theo Rein,Jürgen Zschocke
      Glia 60 2012

      Show Abstract
      22593010
      Valproate alters dopamine signaling in association with induction of par-4 protein expression.
      Saebom Lee,Jaehoon Jeong,Young-Un Park,Yongdo Kwak,Seol Ae Lee,Haeryun Lee,Hyeon Son,Sang Ki Park
      PloS one 7 2012

      Show Abstract
      23029138
      Local microenvironment provides important cues for cell differentiation in lingual epithelia.
      Feng Li,Mingliang Zhou
      PloS one 7 2012

      Show Abstract
      22514735
      NFκB and AP-1 drive human myometrial IL8 expression.
      Shirin Khanjani,Vasso Terzidou,Mark R Johnson,Phillip R Bennett
      Mediators of inflammation 2012 2012

      Show Abstract
      22685373
      HIF2α-Sp1 interaction mediates a deacetylation-dependent FVII-gene activation under hypoxic conditions in ovarian cancer cells.
      Shiro Koizume,Shin Ito,Etsuko Miyagi,Fumiki Hirahara,Yoshiyasu Nakamura,Yuji Sakuma,Hitoshi Osaka,Yasuo Takano,Wolfram Ruf,Yohei Miyagi
      Nucleic acids research 40 2012

      Show Abstract
      22402494
      miR-206 integrates multiple components of differentiation pathways to control the transition from growth to differentiation in rhabdomyosarcoma cells.
      Kyle L Macquarrie,Zizhen Yao,Janet M Young,Yi Cao,Stephen J Tapscott
      Skeletal muscle 2 2012

      Show Abstract
      22541669
      Late-onset Increases in Oxidative Stress and Other Tumorigenic Activities and Tumors With a Ha-ras Mutation in the Liver of Adult Male C3H Mice Gestationally Exposed to Arsenic.
      Keiko Nohara,Yukiyo Tateishi,Takehiro Suzuki,Kazuyuki Okamura,Hikari Murai,Shota Takumi,Fumihiko Maekawa,Noriko Nishimura,Masuko Kobori,Takaaki Ito
      Toxicological sciences : an official journal of the Society of Toxicology 129 2012

      Show Abstract
      22700541
      Chromatin Modulation at the FLO11 Promoter of Saccharomyces cerevisiae by HDAC and Swi/Snf Complexes.
      Ramón R Barrales,Philipp Korber,Juan Jimenez,José I Ibeas
      Genetics 191 2012

      Show Abstract
      22542969
      Valproic acid triggers erythro/megakaryocyte lineage decision through induction of GFI1B and MLLT3 expression.
      Roberta Zini,Ruggiero Norfo,Francesco Ferrari,Elisa Bianchi,Simona Salati,Valentina Pennucci,Giorgia Sacchi,Chiara Carboni,Giovanni Battista Ceccherelli,Enrico Tagliafico,Sergio Ferrari,Rossella Manfredini,
      Experimental hematology 40 2012

      Show Abstract
      22885124

      FAQ

      QuestionAnswer
      How was this antibody made? What modifications will it recognize?The Anti-acetyl-Histone H4 was raised against the tetra acetylated H4. This antibody will recognize intermediately acetylated H4, but we are unsure what the reactivity for the antibody is against tetra-acetylated H4. We know it will not recognize acetylation on lysine 16, however it does recognize acetylation on the other three sites. A protein does not require acetylation on all four sites for this antibody to recognize it.