06-1047 | Anti-IRF4 Antibody, NT

Immunocytochemistry:
Representative lot data.
Confocal fluorescent analysis of PC3 cells using Anti-IRF4. Actin filaments have been labeled with Alexa Fluor 488-Phalloidin (Green), and nuclei are stained with DAPI (Blue) (left image). Anti-IRF4 shows positive cytosolic and nuclear staining (Red) (right image).
Immunocytochemistry:
Representative lot data.
Confocal fluorescent analysis of PC3 cells u
06-1047
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, H, M, EqICC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number 06-1047
      Synonyms interferon regulatory factor 4 Lymphocyte-specific interferon regulatory factor Multiple myeloma oncogene
      Description Anti-IRF4 Antibody, NT
      Background Information Interferon regulatory factor 4, also known as IRF4, belongs to the IRF family. IRF4 encodes a 450 amino acid polypeptide with a predicted molecular weight of 51.6 kDa (Grossman 1996). The IRF4 gene is expressed in the spleen, lymphocytes and melanocytes, and is induced in to T cells after cross linking with the T cell receptor. IRF4 is a transcriptional activator that binds to the interferon stimulated response element (ISRE) of the MHC class I promoter. IRF4 is essential for mature T and B lymphocyte function. IRF4 interacts with MYD88 to prevent the interaction of IRF5, resulting in negative regulation of Toll Like Receptor (TLR) signaling. High levels of IRF4 have been associated with Epstein Barr Virus (EBV) and may be critical to EBV transformation. Further oncogenic potential has been associated with the involvement of IRF4 with hair color and pigmentation of the skin, which may cause multiple myeloma.
      References
      Product Information
      Format Affinity Purified
      Specificity This antibody recognizes the N-terminal region of IRF4 at and around amino acids 177-194.
      Control
      • Mouse brain lysate.
      Presentation Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      Application Anti-IRF4 Antibody, N-terminus detects level of IRF4 & has been published & validated for use in WB & IC.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application Notes Immunocytochemistry: 1:500 dilution of a previous lot of this antibody was used on immunocytochemistry in PC3 cells.
      Biological Information
      Immunogen species KLH conjugated linear peptide corresponding to the N-terminal region of IRF4.
      Epitope N-Terminus
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Rabbit
      Specificity This antibody recognizes the N-terminal region of IRF4 at and around amino acids 177-194.
      Species Reactivity Rat Human Mouse Horse
      Species Reactivity Note Tested to react with human and mouse. Predicted to react with rat and horse based on sequence homology.
      Antibody Type Polyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NF-EM5
      • IRF-4
      • LSIRF
      • MUM1
      Purification Method Affinity Purfied
      UniProt Number
      UniProt Summary Function: Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.
      Subunit structure: Interacts with SPIB and DEF6. Ref.5 Ref.6
      Subcellular location: Nucleus.
      Tissue specificity: Lymphoid cells.
      Induction: Not induced by interferons.
      Involvement in disease: A chromosomal aberration involving IRF4 may be a cause of multiple myeloma [MIM:254500]. Translocation t(6;14)(p25;q32) with the IgH locus.
      Sequence similarities: Belongs to the IRF family.
      Contains 1 tryptophan pentad repeat DNA-binding domain.

      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Evaluated by western blot on mouse brain lysate.
      Western Blot Analysis: 0.5 μg/mL of this antibody detected IRF4 in 10 μg of mouse brain lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size 100 µg
      Transport Information
      Supplemental Information
      Specifications