05-939 | Anti-LSD1/BHC110 Antibody

HeLa cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-LSD1/BHC110 (1:2000 dilution). Arrow indicates LSD1/BHC110 (~110kDa).
HeLa cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti
05-939
100  µL
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HChIP, IF, IHC, IP, WBMAscitesMonoclonal Antibody
      Description
      Catalogue Number 05-939
      Brand Family Upstate
      Synonyms lysine-specific histone demethylase 1 BRAF35-HDAC complex protein BHC110 FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit Flavin-containing amine oxidase domain-containing protein 2 amine oxidase (flavin containing) domain 2 lysine (K)-specific demethylase 1
      Trade Name
      • Upstate
      Description Anti-LSD1/BHC110 Antibody
      Background Information Histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and tri-methylted 'Lys-4' of histone H3. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. May also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.
      References
      Product Information
      Format Ascites
      Specificity Recognizes LSD1, Mr 110 kDa.
      Control
      • HeLa cell lysate.
      Presentation Mouse monoclonal in buffer containing 0.05% sodium azide and 30% glycerol.
      Applications
      Application Anti-LSD1/BHC110 Antibody is a Mouse Monoclonal Antibody for detection of LSD1/BHC110 also known as BRAF35-HDAC complex protein BHC110, lysine (K)-specific demethylase 1 & has been validated in ChIP, IF, IHC, IP & WB.
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • Immunofluorescence
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application Notes Chromatin Immunoprecipitation:
      This antibody has been reported by an independent laboratory to immunoprecipitate LSD1 from chromatin.

      Immunoprecipitation:
      Recommended.

      Immunohistochemistry:
      This antibody has been reported by an independent laboratory to detect LSD1 using paraffin-embedded tissues. (Metzger, E., 2005)

      Immunofluorescence:
      Recommended.
      Biological Information
      Immunogen species GST fusion protein corresponding to residues 23-138 of human LSD1.
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Mouse
      Specificity Recognizes LSD1, Mr 110 kDa.
      Species Reactivity Mouse Human
      Species Reactivity Note Human and mouse.
      Antibody Type Monoclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternate splicing has been observed; however, not all transcript variants have been fully characterized.
      Gene Symbol
      • LSD1
      • AOF2
      • KIAA0601
      • BHC110
      Purification Method Unpurified
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: O60341 # Histone demethylase that specifically demethylates 'Lys- 4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and tri- methylated 'Lys-4' of histone H3. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. May also demethylate 'Lys- 9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.
      COFACTOR: FAD.
      SIZE: 852 amino acids; 92903 Da
      SUBUNIT: Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, AOF2/LSD1, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. In the complex, RCOR1/CoREST strongly enhances the demethylase activity and protects it from the proteasome while PHF21A/BHC80 inhibits the demethylase activity. Interacts with the androgen receptor (AR).
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Ubiquitously expressed.
      DOMAIN: SwissProt: O60341 The SWIRN domain may act as an anchor site for a histone tail.
      SIMILARITY: Belongs to the flavin monoamine oxidase family. & Contains 1 SWIRM domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Routinely evaluated by western blot.

      Western Blot Analysis:
      1:500-1:2000 dilution of this lot detected LSD1 in RIPA lysates from HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Qty/Pck 100 µL
      Material Size 100
      Material Package µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-LSD1/BHC110 2475731
      Anti-LSD1/BHC110 - 2276335 2276335
      Millipore - COA - Anti-LSD1/BHC110 - 2039870 2039870
      Millipore - COA - Anti-LSD1/BHC110 - 30522 30522
      Millipore - COA - Anti-LSD1/BHC110 - 33455 33455
      Millipore - COA - Anti-LSD1/BHC110 - JBC1361937 JBC1361937
      Millipore - COA - Anti-LSD1/BHC110 - NG1904282 NG1904282

      References

      Reference overviewApplicationPub Med ID
      LSD1 demethylates repressive histone marks to promote androgen-receptor-dependent transcription.
      Metzger, Eric, et al.
      Nature, 437: 436-9 (2005) 2005

      Show Abstract
      16079795
      Histone demethylation mediated by the nuclear amine oxidase homolog LSD1.
      Shi, Yujiang, et al.
      Cell, 119: 941-53 (2004) 2004

      Show Abstract
      Immunoblotting (Western)15620353
      A candidate X-linked mental retardation gene is a component of a new family of histone deacetylase-containing complexes.
      Hakimi, Mohamed-Ali, et al.
      J. Biol. Chem., 278: 7234-9 (2003) 2003

      Show Abstract
      12493763