05-904 | Anti-HA Tag Antibody

Western Blot Analysis
Lysates from untransfected (lane 1) and transfected (lane 2) HEK293 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with Anti-HA Tag (0.5 μg/mL).
Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

Arrow indicates HA-tagged protein (~49 kDa). The apparent molecular weight is related to the tagged protein.
Western Blot Analysis
Lysates from untransfected (lane 1) and transfected (lane 2) HEK293 cells
05-904
200 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      VrtICC, WB, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number 05-904
      Brand Family Upstate
      Trade Name
      • Upstate
      Description Anti-HA Tag Antibody
      Overview Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
      Background Information Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
      References
      Product Information
      Format Purified
      Specificity Recognizes HA tagged proteins overexpressed in cells, including both amino or carboxy-termini of targeted proteins in transfected mammalian cells.
      Control
      • RIPA lysates from HEK293 cells.
      Presentation Purified mouse monoclonal IgG3 in buffer containing PBS, pH 7.4 containing 0.05% sodium azide.
      Applications
      Application Detect HA Tag using this Anti-HA Tag Antibody validated for use in IC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      • Immunoprecipitation
      Application Notes Immunoprecipitation:
      5 μg/mL of his antibody has been reported by an independent laboratory to immunoprecipitate recombinant HA-tagged fusion protein.

      Immunocytochemistry:
      5 μg/mL of this antibody has been reported by an independent laboratory to immunostain HA-tagged fusion protein.
      Biological Information
      Immunogen species KLH-conjugated, synthetic peptide corresponding to amino acids 98-106 of influenza hemaglutinin.
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Mouse
      Specificity Recognizes HA tagged proteins overexpressed in cells, including both amino or carboxy-termini of targeted proteins in transfected mammalian cells.
      Isotype IgG3
      Species Reactivity Vertebrates
      Species Reactivity Note Most common vertebrate species tested. Reactivity with other species has not been confirmed.
      Antibody Type Monoclonal Antibody
      Purification Method Protein A purfied
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Routinely evaluated by western blot of recombinant protein containing the HA-tag at the amino terminus in RIPA lysates from HEK293 cells.

      Western Blot Analysis:
      0.5-2 μg/mL of this lot detected a recombinant protein containing the HA-tag at the amino terminus in RIPA lysates from HEK293 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size 200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-HA Tag (mouse monoclonal IgG3) - DAM1462563 DAM1462563
      Anti-HA Tag - 2391105 2391105
      Anti-HA Tag - 2452520 2452520
      Anti-HA Tag - 0606033551 0606033551
      Anti-HA Tag - 0608038421 0608038421
      Anti-HA Tag - 0610043994 0610043994
      Anti-HA Tag - 0611045941 0611045941
      Anti-HA Tag - 1984837 1984837
      Anti-HA Tag - 2044818 2044818
      Anti-HA Tag - 2064516 2064516
      Anti-HA Tag - 2190633 2190633
      Anti-HA Tag - 2276334 2276334
      Anti-HA Tag - 2491216 2491216
      Anti-HA Tag - 30384 30384
      Anti-HA Tag - 32436 32436
      Anti-HA Tag - 33351 33351
      Anti-HA Tag - DAM1394781 DAM1394781
      Anti-HA Tag - DAM1676171 DAM1676171
      Anti-HA Tag - DAM1690645 DAM1690645
      Anti-HA Tag - DAM1718050 DAM1718050
      Anti-HA Tag - DAM1770288 DAM1770288
      Anti-HA Tag - DAM1794298 DAM1794298
      Anti-HA Tag - JBC1381963 JBC1381963
      Anti-HA Tag - JBC1879872 JBC1879872
      Anti-HA Tag - JBC1900557 JBC1900557
      Monoclonal Antibody - JBC1903651 JBC1903651

      References

      Reference overviewPub Med ID
      A RASSF1A polymorphism restricts p53/p73 activation and associates with poor survival and accelerated age of onset of soft tissue sarcoma.
      Karen S Yee,Lukasz Grochola,Garth Hamilton,Anna Grawenda,Elisabeth E Bond,Helge Taubert,Peter Wurl,Gareth L Bond,Eric O'Neill
      Cancer research 72 2012

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      22389451
      Neutrophils activate alveolar macrophages by producing caspase-6-mediated cleavage of IL-1 receptor-associated kinase-M.
      Kobayashi H, Nolan A, Naveed B, Hoshino Y, Segal LN, Fujita Y, Rom WN, Weiden MD
      J Immunol 186 403-10. Epub 2010 Nov 22. 2011

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      21098228
      A region of the nucleosome required for Multiple types of transcriptional silencing in saccharomyces cerevisiae.
      Prescott ET, Safi A, Rusche LN
      Genetics 188 535-48. Epub 2011 May 5. 2011

      Show Abstract
      21546544
      Involvement of p21 (waf1) in merlin deficient sporadic vestibular schwannomas.
      Wu H, Chen Y, Wang Z, Li W, Li J, Zhang L, Lu Y
      Neuroscience 2010

      Show Abstract
      20600642
      TBC1D24, an ARF6-interacting protein, is mutated in familial infantile myoclonic epilepsy.
      Falace, Antonio, et al.
      Am. J. Hum. Genet., 87: 365-70 (2010) 2010

      Show Abstract
      20727515
      Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems
      Terpe, K
      Appl Microbiol Biotechnol, 60:523-33 (2003) 2003

      12536251