05-747 | Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) Antibody, rabbit monoclonal

WB: 20 micrograms of untreated (Lanes 1 & 3) and B-Raf phosphorylated (Lanes 2 & 4) PC-12 cell lysate was probed with a 1:5000 dilution of Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) (Lanes 1 & 2) or total 1:1000 dilution of Anti-MEK1 (#06-269, Lanes 3 & 4). Arrow indicates MEK1 (~45kDa).
WB: 20 micrograms of untreated (Lanes 1 & 3) and B-Raf phosphorylated (Lanes 2 & 4) PC-12 cell lysat
05-747
100 µL  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, MWBRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number 05-747
      Brand Family Upstate
      Trade Name
      • Upstate
      Description Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) Antibody, rabbit monoclonal
      References
      Product Information
      Format Purified
      Specificity phosphorylated MEK1/MEK2
      Presentation 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      Application Detect phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) using this Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) Antibody validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      Immunogen species Peptide containing DpSMANpSF in which pS corresponds to phosphorylated amino acids 218 and 222 of human MEK1
      Host Rabbit
      Specificity phosphorylated MEK1/MEK2
      Isotype IgG
      Species Reactivity Human Rat Mouse
      Antibody Type Monoclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
      Gene Symbol
      • MAP2K1
      • MEK1
      • MAPKK1
      • MKK1
      • PRKMK1
      Modifications
      • Phosphorylation
      Purification Method Protein A purfied
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: Q02750 # Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
      SIZE: 393 amino acids; 43439 Da
      SUBUNIT: Interacts with MORG1 (By similarity). Interacts with Yersinia yopJ.
      PTM: Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity. & Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
      DISEASE: SwissProt: Q02750 # Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio- cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
      SIMILARITY: SwissProt: Q02750 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. & Contains 1 protein kinase domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance routinely evaluated by immunoblot on RIPA lysates from PC-12 cells that have been treated with B-Raf
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions 2 years at -20°C
      Packaging Information
      Material Size 100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) - 26733 26733
      Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) - 29669 29669
      Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) - 29726 29726
      Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) - 30004 30004

      References

      Reference overviewPub Med ID
      The 5-HT 2A serotonin receptor enhances cell viability, affects cell cycle progression and activates MEK-ERK1/2 and JAK2-STAT3 signalling pathways in human choriocarcinoma cell lines.
      Oufkir T, Arseneault M, Sanderson JT, Vaillancourt C
      Placenta 31 439-47. Epub 2010 Mar 25. 2010

      Show Abstract
      20338635
      AKAP-Lbc enhances cyclic AMP control of the ERK1/2 cascade.
      F Donelson Smith,Lorene K Langeberg,Cristina Cellurale,Tony Pawson,Deborah K Morrison,Roger J Davis,John D Scott
      Nature cell biology 12 2010

      Show Abstract Full Text Article
      21102438
      Negative regulation of MAPKK by phosphorylation of a conserved serine residue equivalent to Ser212 of MEK1.
      Gopalbhai, Kailesh, et al.
      J. Biol. Chem., 278: 8118-25 (2003) 2003

      Show Abstract
      12506122
      Growth hormone stimulates phosphorylation and activation of elk-1 and expression of c-fos, egr-1, and junB through activation of extracellular signal-regulated kinases 1 and 2.
      Hodge, C, et al.
      J. Biol. Chem., 273: 31327-36 (1998) 1998

      Show Abstract
      9813041