05-716 | Anti-Myosin Heavy Chain Antibody, clone A4.1025

WB: C2C12 cell lysate was probed with anti-Myosin Heavy Chain (MyHC) (1:1000 dilution).
05-716
200 µL  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Rb, H, R, M, Zebrafish, DrWB, IHCMAscitesMonoclonal Antibody
      Description
      Catalogue Number 05-716
      Brand Family Upstate
      Synonyms myosin, heavy polypeptide 1, skeletal muscle, adult Myosin heavy chain 1 Myosin heavy chain 2x Myosin heavy chain IIx/d Myosin heavy chain, skeletal muscle, adult 1 myosin, heavy chain 1, skeletal muscle, adult
      Trade Name
      • Upstate
      Description Anti-Myosin Heavy Chain Antibody, clone A4.1025
      Background Information Myosin is one of the two major components of muscle tissue responsible for contraction. In muscle cells myosin is arranged as thick filamentous structures that are arranged in parallel to thin filamentous structures composed of actin. Muscle contraction is actuated by the coupling and uncoupling of thin and thick filaments through a chemical linkage mediated by ATP. The coupling and uncoupling is arranged such that the filaments slide in one direction when coupled and in reverse direction when uncoupled or released. Myosin and actin also serve to facilitate motility in non-muscle cells such as in slime molds where shorter yet similar filamentous structures work together. An increasing number of different myosins are being described. Myosin I is a low molecular weight myosin found in protozoa and does not self-assemble. Myosin II is the classic form found in muscle cells that self assemble into thick bundles. Myosin II is a multimeric protein that is composed of two heavy chains and two light chains.
      References
      Product Information
      Format Ascites
      Specificity This antibody recognizes myosin heavy chain, Mr ~220 kDa. Immunoblotting with muscle cell lysates will often show several close bands from different myosin heavy chain genes, as well as degradation products.
      Control
      • RIPA lysates from C2C12 cells
      Presentation Unpurified mouse ascites monoclonal IgG in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol. Liquid at -20°C.
      Applications
      Application Anti-Myosin Heavy Chain Antibody, clone A4.1025 is an antibody against Myosin Heavy Chain for use in IH & WB.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application Notes Immunohistochemistry:
      This antibody has been reported by an independent laboratory to detect myosin heavy chain in zebrafish embryos and skeletal muscle sections fixed with 4% paraformaldehyde in a previous lot (Blagden, C.S., 1997; Cho, M., 1994).
      Biological Information
      Immunogen species Partially purified protein from adult human skeletal muscle myosin. Clone A4.1025.
      Clone A4.1025
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Mouse
      Specificity This antibody recognizes myosin heavy chain, Mr ~220 kDa. Immunoblotting with muscle cell lysates will often show several close bands from different myosin heavy chain genes, as well as degradation products.
      Isotype IgG
      Species Reactivity Rabbit Human Rat Mouse Zebrafish Drosophila
      Species Reactivity Note Human, mouse, rat, rabbit, zebrafish and Drosophila.
      Antibody Type Monoclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development.
      Gene Symbol
      • MYHSA1
      • MYH1
      • MyHC-2X/D
      • MGC133384
      • MYHa
      • MyHC-IIx/d
      • MyHC-2x
      • Myosin-1
      Purification Method Unpurified
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: P12882 # Muscle contraction.
      SIZE: 1939 amino acids; 223115 Da
      SUBUNIT: Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).
      SUBCELLULAR LOCATION: Cytoplasm, myofibril. Note=Thick filaments of the myofibrils.
      DOMAIN: SwissProt: P12882 The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
      SIMILARITY: Contains 1 IQ domain. & Contains 1 myosin head-like domain.
      MISCELLANEOUS: Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Routinely evaluated by Western Blot on RIPA lysates from C2C12 cells.

      Western Blot Analysis:
      1:1000-1:5000 dilution of this lot detected Myosin Heavy Chain (MyHC) in RIPA lysates from Rabbit skeletal muscle cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at -20ºC from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap.

      Handling Recommendations:
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size 200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-Myosin Heavy Chain (MyHC), clone A4.1025 (mouse ascites) - DAM1408805 DAM1408805
      Anti-Myosin Heavy Chain, clone A4.1025 2472931
      Anti-Myosin Heavy Chain, clone A4.1025 - 0702051566 0702051566
      Anti-Myosin Heavy Chain, clone A4.1025 - 24512 24512
      Anti-Myosin Heavy Chain, clone A4.1025 - 2500673 2500673
      Anti-Myosin Heavy Chain, clone A4.1025 - DAM1394773 DAM1394773
      Anti-Myosin Heavy Chain, clone A4.1025 - JBC1361185 JBC1361185

      References

      Reference overviewApplicationPub Med ID
      Effects of doxorubicin cancer therapy on autophagy and the ubiquitin-proteasome system in long-term cultured adult rat cardiomyocytes.
      Polychronis Dimitrakis,Maria-Iris Romay-Ogando,Francesco Timolati,Thomas M Suter,Christian Zuppinger
      Cell and tissue research 350 2012

      Show Abstract
      22864983
      Comparison of various kinds of bone marrow stem cells for the repair of infarcted myocardium: single clonally purified non-hematopoietic mesenchymal stem cells serve as a superior source.
      Shaoheng Zhang, Junbo Ge, Aijun Sun, Danling Xu, Juying Qian, Jinyi Lin, Yan Zhao, Haifeng Hu, Ying Li, Keqiang Wang, Yunzeng Zou
      Journal of cellular biochemistry 99 1132-47 2006

      Show Abstract
      16795039
      Evidence for differential post-translational modifications of slow myosin heavy chain during murine skeletal muscle development
      Maggs, A. M., et al
      J Muscle Res Cell Motil, 21:101-13 (2000) 2000

      Immunoblotting (Western), Immunohistochemistry (Tissue)10961835
      Notochord induction of zebrafish slow muscle mediated by Sonic hedgehog
      Blagden, C. S., et al
      Genes Dev, 11:2163-75 (1997) 1997

      Immunohistochemistry (Tissue)9303533
      Fast myosin heavy chains expressed in secondary mammalian muscle fibers at the time of their inception
      Cho, M, et al
      J Cell Sci, 107 ( Pt 9):2361-71 (1994) 1994

      7531198