05-587 | Anti-phospho-Adducin (Ser724) Antibody, clone pADD

WB: Non-stimulated (lane 1) and PMA-stimulated (lane 2) human red blood cell lysate, was probed with anti-phospho-Adducin, clone pADD (S724) (0.5μg/ml).
WB: Non-stimulated (lane 1) and PMA-stimulated (lane 2) human red blood cell lysate, was probed with
05-587
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, H, MICC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number 05-587
      Brand Family Upstate
      Trade Name
      • Upstate
      Description Anti-phospho-Adducin (Ser724) Antibody, clone pADD
      References
      Product Information
      Format Purified
      Specificity phospho-Adducin isoforms
      Presentation 0.7M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide and 30% glycerol.
      Applications
      Application Detect phospho-Adducin (Ser724) using this Anti-phospho-Adducin (Ser724) Antibody, clone pADD validated for use in IP, WB & IC.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      Immunogen species peptide (FRTP[pS]FLKK) corresponding to amino acids 720-728 of rat phospho-α-Adducin (Ser724)
      Clone pADD
      Host Mouse
      Specificity phospho-Adducin isoforms
      Isotype IgG
      Species Reactivity Rat Human Mouse
      Antibody Type Monoclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.
      Gene Symbol
      • MGC3339
      • ADD1
      • ADDA
      • Alpha-adducin
      • MGC44427
      Modifications
      • Phosphorylation
      Purification Method Protein G chromatography
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: P35611 # Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
      SIZE: 737 amino acids; 80955 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit or an alpha and a gamma subunit. Binds ROCK1.
      SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
      TISSUE SPECIFICITY: Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
      DOMAIN: SwissProt: P35611 Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
      PTM: The N-terminus is blocked.
      SIMILARITY: Belongs to the aldolase class II family. Adducin subfamily.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance routinely evaluated by immunoblot on lysates from human red blood cells stimulated with PMA
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions 2 years at -20°C
      Packaging Information
      Material Size 100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Adducin (Ser724), clone pADD - 20199 20199
      Anti-phospho-Adducin (Ser724), clone pADD - 27688 27688

      References

      Reference overviewPub Med ID
      Src protein-tyrosine kinase structure and regulation.
      Roskoski, Robert
      Biochem. Biophys. Res. Commun., 324: 1155-64 (2004) 2004

      Show Abstract
      15504335
      Role of STATs as downstream signal transducers in Src family kinase-mediated tumorigenesis
      Silva, Corinne M
      Oncogene, 23:8017-23 (2004) 2004

      15489919