05-201 | Anti-Fas Antibody (human, activating), clone CH11

05-201
50 µg  
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      Overview

      Key Spec Table

      Species Reactivity Key Applications Host Format Antibody Type
      HWB,FC,ICCMAffinity PurifiedMonoclonal Antibody
      Description
      Catalogue Number 05-201
      Brand Family Upstate
      Synonyms tumor necrosis factor receptor superfamily, member 6 APO-1 cell surface antigen CD95 antigen Fas (TNF receptor superfamily, member 6) Fas AMA Fas antigen apoptosis antigen 1
      Trade Name
      • Upstate
      Description Anti-Fas Antibody (human, activating), clone CH11
      Background Information Fas/APO-1/CD95 (36 kDa) is a member of the tumor necrosis factor (TNF) receptor superfamily, a family of transmembrane receptors. Fas has been shown to be an important mediator of apoptotic cell death, as well as being involved in inflammation. Binding of the Fas ligand (Fas-L) induces trimerization of Fas in the target cell membrane. Activation of Fas causes the recruitment of Fas-associated protein with death domain (FADD) via interactions between the death domains of Fas and FADD. Procaspase 8 binds to Fas-bound FADD via interactions between the death effector domains (DED) of FADD and pro-caspase 8 leading to the activation of caspase 8. Activated caspase 8 cleaves (activates) other procaspases, in effect beginning a caspase cascade that ultimately leads to apoptosis. Caspases cleave nuclear lamins, causing the nucleus to break down and lose its normal structure. Fas-induced apoptosis can be effectively blocked at several stages by either FLICE-inhibitory protein (FLIP), by Bcl-2, or by the cytokine response modifier A (CrmA).

      Biological Activity
      The antibody demonstrates cytolytic activity on human cells that express Fas. Murine WR19L cells and L929 cells transfected with cDNA encoding human Fas undergo apoptosis in response to this antibody.
      References
      Product Information
      Format Affinity Purified
      Specificity This antibody recognizes the human cell surface antigen Fas, Mr 43 kDa expressed in various human cells, including myeloid cells, T lympho-blastoid cells, and diploid fibroblasts.
      Control
      • Human liver tumor, human breast tumor or Jurkat whole cell lysate, Raji cell lysate.
      Presentation Purified mouse monoclonal IgM in buffer containing PBS, pH 7.2, with 50% glycerol. Liquid at -20ºC.
      Applications
      Application Detect Fas using this Anti-Fas Antibody (human, activating), clone CH11 validated for use in FC, IC & WB.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • Immunocytochemistry
      Application Notes Western Blot:
      0.5-2 μg/mL of a previous lot detected Fas in a Raji cell lysate.
      Immunocytochemistry:
      5-10 μg/mL of a previous lot detected Fas on HeLa cells fixed with 4% formalin/2% acetic acid.
      Flow cytometry:
      A previous lot of was tested by an independent laboratory using 20 μg/mL of anti-Fas, clone CH11 (Yonehara, S., 1989; Kobayashi, N., 1990).
      Biological Information
      Immunogen FS-7 (human diploid fibroblast cell line). Clone CH-11.
      Clone CH11
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Mouse
      Specificity This antibody recognizes the human cell surface antigen Fas, Mr 43 kDa expressed in various human cells, including myeloid cells, T lympho-blastoid cells, and diploid fibroblasts.
      Isotype IgM
      Species Reactivity Human
      Species Reactivity Note Proven to react with human.
      This antibody does not recognize TNF, and does not cross-react with mouse Fas. Fas ligand will induce apoptosis in human, mouse and rat systems.
      Antibody Type Monoclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.
      Gene Symbol
      • APO-1
      • FAS
      • OTTHUMP00000059646
      • LFG
      • FASTM
      • ALPS1A
      • TNFRSF6
      • CD95
      • FAS1
      • FAIM2
      • APT1
      • NMP35
      Purification Method Immunoaffinity chromatography
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: P25445 # Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
      SIZE: 335 amino acids; 37732 Da
      SUBUNIT: Binds DAXX. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD (By similarity). Binds RIPK1 and FAIM2. Interacts with BRE.
      SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted. & Isoform 3: Secreted. & Isoform 4: Secreted. & Isoform 5: Secreted. & Isoform 6: Secreted.
      TISSUE SPECIFICITY: Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
      DOMAIN: SwissProt: P25445 Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
      DISEASE: SwissProt: P25445 # Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
      SIMILARITY: Contains 1 death domain. & Contains 3 TNFR-Cys repeats.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Routinely evaluated by demonstrating cytolytic activity on human cells that express Fas. Murine WR19L cells and L929 cells transfected with cDNA encoding human Fas undergo apoptosis in response to this antibody.

      Apoptosis Assay Analysis:
      15-20 µg/mL of this lot maximally induced apoptosis of human Jurkat cells with 83% mortality after 24 hours of treatment.
      Sales Restrictions This product is not available for sale in Japan.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at -20°C from date of receipt. For maximum recovery of the product, centrifuge the original vial prior to removing the cap.
      Packaging Information
      Material Size 50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Languages
      Deutschland

      Certificates of Analysis

      TitleLot Number
      Anti-Fas (human, activating) 2453649
      Anti-Fas (human, activating), clone CH11 - 2145050 2145050
      Anti-Fas (human, activating), clone CH11 - 2390345 2390345
      Anti-Fas (human, activating), clone CH11 - 2397046 2397046
      Anti-Fas (human, activating), clone CH11 2470339
      Anti-Fas (human, activating), clone CH11 - 0704056775 0704056775
      Anti-Fas (human, activating), clone CH11 - 16630 16630
      Anti-Fas (human, activating), clone CH11 - 16890 16890
      Anti-Fas (human, activating), clone CH11 - 17129 17129
      Anti-Fas (human, activating), clone CH11 - 17367 17367
      Anti-Fas (human, activating), clone CH11 - 17669 17669
      Anti-Fas (human, activating), clone CH11 - 18059 18059
      Anti-Fas (human, activating), clone CH11 - 18297 18297
      Anti-Fas (human, activating), clone CH11 - 18787 18787
      Anti-Fas (human, activating), clone CH11 - 19511 19511
      Anti-Fas (human, activating), clone CH11 - 1971247 1971247
      Anti-Fas (human, activating), clone CH11 - 1984664 1984664
      Anti-Fas (human, activating), clone CH11 - 1987444 1987444
      Anti-Fas (human, activating), clone CH11 - 19912 19912
      Anti-Fas (human, activating), clone CH11 - 1994896 1994896
      Anti-Fas (human, activating), clone CH11 - 2026375 2026375
      Anti-Fas (human, activating), clone CH11 - 2060951 2060951
      Anti-Fas (human, activating), clone CH11 - 20831 20831
      Anti-Fas (human, activating), clone CH11 - 21471 21471
      Anti-Fas (human, activating), clone CH11 - 21844 21844
      Anti-Fas (human, activating), clone CH11 - 2219267 2219267
      Anti-Fas (human, activating), clone CH11 - 22819 22819
      Anti-Fas (human, activating), clone CH11 - 2283805 2283805
      Anti-Fas (human, activating), clone CH11 - 23212 23212
      Anti-Fas (human, activating), clone CH11 - 2326275 2326275
      Anti-Fas (human, activating), clone CH11 - 2334465 2334465
      Anti-Fas (human, activating), clone CH11 - 23654 23654
      Anti-Fas (human, activating), clone CH11 - 23933 23933
      Anti-Fas (human, activating), clone CH11 - 24775 24775
      Anti-Fas (human, activating), clone CH11 - 2488729 2488729
      Anti-Fas (human, activating), clone CH11 - 2519251 2519251
      Anti-Fas (human, activating), clone CH11 - 26201 26201
      Anti-Fas (human, activating), clone CH11 - 26693 26693
      Anti-Fas (human, activating), clone CH11 - 28116 28116
      Anti-Fas (human, activating), clone CH11 - 29894 29894
      Anti-Fas (human, activating), clone CH11 - 32016 32016
      Anti-Fas (human, activating), clone CH11 - 33371 33371
      Anti-Fas (human, activating), clone CH11 - 33574 33574
      Anti-Fas (human, activating), clone CH11 - DAM1403940 DAM1403940
      Anti-Fas (human, activating), clone CH11 - DAM1493394 DAM1493394
      Anti-Fas (human, activating), clone CH11 - DAM1493394 DAM1493394
      Anti-Fas (human, activating), clone CH11 - DAM1612178 DAM1612178
      Anti-Fas (human, activating), clone CH11 - DAM1698714 DAM1698714
      Anti-Fas (human, activating), clone CH11 - DAM1764379 DAM1764379
      Anti-Fas (human, activating), clone CH11 - DAM1770262 DAM1770262
      Anti-Fas (human, activating), clone CH11 - JBC1381916 JBC1381916
      Anti-Fas (human, activating), clone CH11 - JBC1381916 JBC1381916
      Anti-Fas (human, activating), clone CH11 - JBC1394138 JBC1394138
      Anti-Fas (human, activating), clone CH11 - JBC1394138 JBC1394138
      Anti-Fas (human, activating), clone CH11 - NG1852164 NG1852164
      Anti-Fas (human, activating), clone CH11 - NG1869257 NG1869257

      References | 40 Available | See All References

      Reference overviewApplicationSpeciesPub Med ID
      XB130, a novel adaptor protein, promotes thyroid tumor growth.
      Shiozaki, Atsushi, et al.
      Am. J. Pathol., 178: 391-401 (2011) 2011

      Show Abstract
      21224076
      NF-κB-dependent cytokine secretion controls Fas expression on chemotherapy-induced premature senescent tumor cells.
      E Crescenzi,F Pacifico,A Lavorgna,R De Palma,E D'Aiuto,G Palumbo,S Formisano,A Leonardi
      Oncogene 30 2011

      Show Abstract
      21278794
      Apoptosis induction in Jurkat cells and sCD95 levels in women's sera are related with the risk of developing cervical cancer.
      Adriana Aguilar-Lemarroy,Jose E Romero-Ramos,Vicente Olimon-Andalon,Georgina Hernandez-Flores,Jose M Lerma-Diaz,Pablo C Ortiz-Lazareno,Gilberto Morgan-Villela,Susana Del Toro-Arreola,Alejandro Bravo-Cuellar,Luis F Jave-Suarez
      BMC cancer 8 2008

      Show Abstract Full Text Article
      18405371
      Co-inherited mutations of Fas and caspase-10 in development of the autoimmune lymphoproliferative syndrome.
      Cerutti, Elisa, et al.
      BMC Immunol., 8: 28 (2007) 2007

      Human17999750
      In vitro assay for the quantitation of apoptosis-induced alterations of nuclear envelope permeability.
      Patricia Grote,Elisa Ferrando-May
      Nature protocols 1 2006

      Show Abstract
      17406565
      Identification and characterization of a novel gene Saf transcribed from the opposite strand of Fas
      Yan, Ming-De, et al
      Hum Mol Genet, 14:1465-74 (2005) 2005

      15829500
      Considering Fas ligand as a target for therapy.
      Linkermann, Andreas, et al.
      Expert Opin. Ther. Targets, 9: 119-34 (2005) 2005

      Show Abstract
      15757486
      Caspase-dependent regulation and subcellular redistribution of the transcriptional modulator YY1 during apoptosis.
      Anja Krippner-Heidenreich, Gesa Walsemann, Maroun J Beyrouthy, Stefanie Speckgens, Regine Kraft, Hubert Thole, Robert V Talanian, Myra M Hurt, Bernhard Lüscher
      Molecular and cellular biology 25 3704-14 2005

      Show Abstract Full Text Article
      15831475
      Elucidation of molecular events leading to neutrophil apoptosis following phagocytosis: cross-talk between caspase 8, reactive oxygen species, and MAPK/ERK activation.
      Zhang, Bin, et al.
      J. Biol. Chem., 278: 28443-54 (2003) 2003

      Show Abstract
      12736263
      Defective function of Fas in patients with type 1 diabetes associated with other autoimmune diseases
      DeFranco, S., et al
      Diabetes, 50:483-8 (2001) 2001

      Apoptosis Assay11246866