05-1050 | 4G10® Platinum, Anti-Phosphotyrosine Antibody (mouse monoclonal cocktail IgG2b)

Lysates from various cells with various treatments to stimulated varying tyrosine phosphorylations on multiple substrates were resolved in parallel by SDS-PAGE and transferred to PVDF membrane. The membranes were probed with one of three different anti-phosphotyrosine antibodies, 4G10® (Cat. 05-321), PY20 (Cat. No. 05-947), or 4G10® Platinum (Cat. No. 05-1050). The various blots show the advantages of the anti-phosphotyrosine monoclonal cocktail, 4G10® Platinum.
Lysates from various cells with various treatments to stimulated varying tyrosine phosphorylations o
05-1050
100 µL  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AIP, WB, ICC, ELISA, IHC, IF, FCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number 05-1050
      Brand Family Upstate
      Trade Name
      • Upstate
      Description 4G10® Platinum, Anti-Phosphotyrosine Antibody (mouse monoclonal cocktail IgG2b)
      Background Information The development of the anti-phosphotyrosine, clone 4G10® in 1989 was a monumental discovery for researchers. 4G10® was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10® is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. To improve on something that hundred have tried and no one has succeeded, we pooled 4G10® with the next most highly regarded anti-phosphotyrosine, clone PY20 to make 4G10® Platinum. PY20 itself is a very poor substitute for 4G10®, but its additive effect allow for a greater level of detection on more substrates that even 4G10® alone was not capable of.
      References
      Product Information
      Format Purified
      Specificity Tyrosine-phosphorylated proteins from all species
      Control
      • Suggested Positive Antigen Control: Cat. # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at -20ºC. The lysate is stable for 6 months at -20ºC. Before use, add 2.5 μL of 2-mercaptoethanol per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for immunoblot analysis.
      Presentation Proprietary mixture of protein G purified mouse monoclonals 4G10® (IgG2b) and PY20 (IgG2b) in 0.1 M Tris-glycine, Ph 7.4, 0.15 M NaCl, 0.05% sodium azide.
      Liquid at 2-8°C.
      Applications
      Application 4G10 Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) detects levels of Phosphotyrosine proteins & has been published & validated for use in WB, ICC, ELISA, IHC, IF, Flow & IP.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      • ELISA
      • Immunohistochemistry
      • Immunofluorescence
      • Flow Cytometry
      Application Notes Immunoprecipitation: 2-4 μL of a previous lot can immunoprecipitate quantitatively the phosphotyrosine containing proteins in the lysate of a confluent culture (10cm dish) of cells expressing an activated tyrosine kinase. To preserve phosphotyrosine, add 0.2 mM sodium orthovanadate to the lysis buffer.
      Biological Information
      Immunogen species 4G10® used Phosphotyramine coupled to KLH and PY20 used phosphotyrosine conjugated to carrier protein
      Host Mouse
      Specificity Tyrosine-phosphorylated proteins from all species
      Isotype IgG2b
      Species Reactivity All
      Antibody Type Monoclonal Antibody
      Modifications
      • Phosphorylation
      Purification Method Protein G-Sepharose™ chromatography
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Evaluated by Western Blot on a modified RIPA lysate from EGF-treated human A431 carcinoma cells.

      Western Blot Analysis: 1:1,000-1:2,000 of this antibody detected tyrosinephosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at from date of receipt.
      Packaging Information
      Material Size 100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 2116028 2116028
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 2023921 2023921
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 1958115 1958115
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 2006534 2006534
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 2189938 2189938
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - DAM1535707 DAM1535707
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - DAM165529 DAM165529
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - DAM1713267 DAM1713267
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - DAM1739309 DAM1739309
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - DAM1823498 DAM1823498
      4G10#174; Platinum, Anti-Phosphotyrosine - DAM1462606 DAM1462606
      4G10#174; Platinum, Anti-Phosphotyrosine - DAM1493437 DAM1493437
      4G10#174; Platinum, Anti-Phosphotyrosine - DAM1514057 DAM1514057
      4G10#174; Platinum, Anti-Phosphotyrosine - DAM1548638 DAM1548638
      4G10#174; Platinum, Anti-Phosphotyrosine - DAM1774799 DAM1774799
      4G10#174; Platinum, Anti-Phosphotyrosine - JBC1870017 JBC1870017
      4G10#174; Platinum, Anti-Phosphotyrosine - JBC1917752 JBC1917752
      4G10#174; Platinum, Anti-Phosphotyrosine - R0709B0207 R0709B0207
      4G10#174; Platinum, Anti-Phosphotyrosine(mouse monoclonal cocktail IgG2b) - DAM1581058 DAM1581058
      4G10® Platinum, Anti-Phosphotyrosine 2475690
      4G10® Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 2392267 2392267
      4G10® Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 2430414 2430414
      4G10® Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 2444121 2444121
      4G10® Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 2297197 2297197
      4G10® Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 2341082 2341082
      4G10® Platinum, Anti-Phosphotyrosine -2517820 2517820

      References | 17 Available | See All References

      Reference overviewSpeciesPub Med ID
      Pro-neural miR-128 is a glioma tumor suppressor that targets mitogenic kinases.
      T Papagiannakopoulos,D Friedmann-Morvinski,P Neveu,J C Dugas,R M Gill,E Huillard,C Liu,H Zong,D H Rowitch,B A Barres,I M Verma,K S Kosik
      Oncogene 31 2012

      Show Abstract
      21874051
      Prolactin-induced activation of phagocyte NADPH oxidase in the teleost fish gilthead seabream involves the phosphorylation of p47phox by protein kinase C.
      Víctor H Olavarría,Jaime E Figueroa,Victoriano Mulero
      Developmental and comparative immunology 36 2012

      Show Abstract
      21884725
      Identification of a Src tyrosine kinase/SIAH2 E3 ubiquitin ligase pathway that regulates C/EBP? expression and contributes to transformation of breast tumor cells.
      Tapasree Roysarkar,Shikha Sharan,Jun Wang,Snehalata A Pawar,Carrie A Cantwell,Peter F Johnson,Deborah K Morrison,Ju-Ming Wang,Esta Sterneck,Tapasree Roy Sarkar,Tapasree Roy Sarkar
      Molecular and cellular biology 32 2012

      Show Abstract
      22037769
      Wnt proteins regulate acetylcholine receptor clustering in muscle cells.
      Bin Zhang,Chuan Liang,Ryan Bates,Yiming Yin,Wen-Cheng Xiong,Lin Mei
      Molecular brain 5 2012

      Show Abstract
      22309736
      The metastasis gene NEDD9 product acts through integrin β3 and Src to promote mesenchymal motility and inhibit amoeboid motility.
      Jessica Ahn,Victoria Sanz-Moreno,Christopher J Marshall
      Journal of cell science 125 2012

      Show Abstract
      22328516
      Omega-3 fatty acids modulate collagen signaling in human platelets.
      Larson, M K, et al.
      Prostaglandins Leukot. Essent. Fatty Acids, 84: 93-8 2011

      Show Abstract
      21177087
      Cytoskeletal polarity mediates localized induction of the heart progenitor lineage.
      James Cooley,Stacia Whitaker,Sarah Sweeney,Scott Fraser,Brad Davidson
      Nature cell biology 13 2011

      Show Abstract
      21785423
      Osmotic stress stimulates phosphorylation and cellular expression of heat shock proteins in rhesus macaque sperm.
      Cole JA, Meyers SA
      Journal of andrology 32 402-10. Epub 2010 Nov 18. 2011

      Show Abstract
      21088232
      GILZ inhibits the mTORC2/AKT pathway in BCR-ABL(+) cells.
      Joha, S, et al.
      Oncogene, (2011) 2011

      Show Abstract
      21804606
      Beta-catenin inhibits T cell activation by selective interference with linker for activation of T cells-phospholipase C-γ1 phosphorylation.
      Driessens G, Zheng Y, Locke F, Cannon JL, Gounari F, Gajewski TF
      J Immunol 186 784-90. Epub 2010 Dec 13. 2011

      Show Abstract
      21149602

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      Product Families

      Phosphotyrosine Signaling Solutions

      Study tyrosine phosphorylation using Millipore's specific and sensitive antibodies, including the popular clone 4G10®, and new 4G10 Platinum, available in various conjugations. See below for more. Learn More >>

      Phosphotyrosine Antibodies

      Millipore’s phosphotyrosine antibodies, including the popular 4G10 clone, demonstrate sensitivity and detect multiple tyrosine phosphorylations on numerous substrates. See below for pTyr antibodies. Learn More >>

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies