04-745 | Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315, rabbit monoclonal

Western Blot Analysis:
Hela cell lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-CCT-beta, clone F39 P7 F11 (0.5 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

Arrow indicates CCTbeta (~60 kDa).
Western Blot Analysis:
Hela cell lysate was resolved by electrophoresis, transferred to PVDF an
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HChIP-seq, WB, DB, MplexRbCulture SupernatantMonoclonal Antibody
      Catalogue Number 04-745
      Brand Family Upstate
      Synonyms Histone H3 (tri methyl K4) H3K4me3
      Trade Name
      • Upstate
      Description Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315, rabbit monoclonal
      Background Information Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure.

      The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      Product Information
      Format Culture Supernatant
      Specificity Histone H3 containing trimethyl-lysine 4 and, to a lesser extent, dimethyl-lysine 4. See COA for specificity data.
      • HeLa acid extract
      Presentation Cultured supernantant in 0.05% sodium azide.
      Application Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315 is a rabbit monoclonal antibody for detection of trimethyl-Histone H3 (Lys4) also known as H3K4me3, Histone H3 (tri methyl K4) & has been validated in WB, ChIP, DB, Mplex, ChIP-seq.
      Key Applications
      • ChIP-seq
      • Western Blotting
      • Dot Blot
      • Multiplexing
      Application Notes WB, ChIP, BD, PIA
      Biological Information
      Immunogen species Peptide containing the sequence [RTtrimKQ] in which lysine 4 is trimethylated on human histone H3
      Clone MC315
      Host Rabbit
      Specificity Histone H3 containing trimethyl-lysine 4 and, to a lesser extent, dimethyl-lysine 4. See COA for specificity data.
      Isotype IgG
      Species Reactivity Human
      Antibody Type Monoclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • H3/t
      • HIST3H3
      • H3FT
      • MGC126886
      • H3t
      • MGC126888
      • H3.4
      • H3T
      • H3/g
      • Methylation
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      TISSUE SPECIFICITY: Expressed in testicular cells.
      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance routinely evaluated by immunoblot on in acid extracted proteins from HeLa cells, but not recombinant unmethylated Histone H3 (Catalog #14-494)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size 100 µL
      Transport Information
      Supplemental Information


      Certificates of Analysis

      TitleLot Number
      Anti-trimethyl-Histone H3 (Lys4) 2462884
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - 2135147 2135147
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - 2326991 2326991
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - 2392291 2392291
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - 2452485 2452485
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - NG1823938 NG1823938
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - 1966931 1966931
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - 1997493 1997493
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - 2020453 2020453
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - 2049822 2049822
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - 2197316 2197316
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - 2276411 2276411
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - 2508912 2508912
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - DAM1494280 DAM1494280
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - DAM154116 DAM154116
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - DAM1554357 DAM1554357
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - DAM1594220 DAM1594220
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - JBC1791502 JBC1791502
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - NG1643014 NG1643014
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - NG1680351 NG1680351
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - NG1717145 NG1717145
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - NG1729919 NG1729919
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - NG1876185 NG1876185
      Anti-trimethyl-Histone H3 (Lys4), clone MC315 - NG1917785 NG1917785

      References | 16 Available | See All References

      Reference overviewPub Med ID
      Endogenous mammalian histone H3.3 exhibits chromatin-related functions during development.
      Bush, Kelly M, et al.
      Epigenetics Chromatin, 6: 7 (2013) 2013

      Show Abstract
      Juxtaposition of heterochromatic and euchromatic regions by chromosomal translocation mediates a heterochromatic long-range position effect associated with a severe neurological phenotype.
      Palma Finelli,Silvia Maria Sirchia,Maura Masciadri,Milena Crippa,Maria Paola Recalcati,Daniela Rusconi,Daniela Giardino,Laura Monti,Francesca Cogliati,Francesca Faravelli,Federica Natacci,Leonardo Zoccante,Bernardo Dalla Bernardina,Silvia Russo,Lidia Larizza
      Molecular cytogenetics 5 2012

      Show Abstract
      ATF4-dependent Regulation of the JMJD3 Gene during Amino Acid Deprivation Can Be Rescued in Atf4-deficient Cells by Inhibition of Deacetylation.
      Jixiu Shan,Lingchen Fu,Mukundh N Balasubramanian,Tracy Anthony,Michael S Kilberg
      The Journal of biological chemistry 287 2012

      Show Abstract
      G9a/GLP histone lysine dimethyltransferase complex activity in the hippocampus and the entorhinal cortex is required for gene activation and silencing during memory consolidation.
      Swati Gupta-Agarwal,Aimee V Franklin,Thomas Deramus,Muriah Wheelock,Robin L Davis,Lori L McMahon,Farah D Lubin
      The Journal of neuroscience : the official journal of the Society for Neuroscience 32 2012

      Show Abstract
      Overexpression of a histone H3K4 demethylase, JMJ15, accelerates flowering time in Arabidopsis.
      Hongchun Yang,Huixian Mo,Di Fan,Ying Cao,Sujuan Cui,Ligeng Ma
      Plant cell reports 31 2012

      Show Abstract
      Epigenetic inactivation of the MiR-124-1 in haematological Malignancies.
      Wong KY, So CC, Loong F, Chung LP, Lam WW, Liang R, Li GK, Jin DY, Chim CS
      PloS one 6 e19027. 2011

      Show Abstract Full Text Article
      A region of the nucleosome required for Multiple types of transcriptional silencing in saccharomyces cerevisiae.
      Prescott ET, Safi A, Rusche LN
      Genetics 188 535-48. Epub 2011 May 5. 2011

      Show Abstract
      Epigenetic switch involved in activation of pioneer factor FOXA1-dependent enhancers.
      Sérandour AA, Avner S, Percevault F, Demay F, Bizot M, Lucchetti-Miganeh C, Barloy-Hubler F, Brown M, Lupien M, Métivier R, Salbert G, Eeckhoute J
      Genome Res 2011

      Show Abstract
      Regulation of TCRβ Allelic Exclusion by Gene Segment Proximity and Accessibility.
      Kondilis-Mangum HD, Shih HY, Mahowald G, Sleckman BP, Krangel MS
      Journal of immunology (Baltimore, Md : 1950) 2011

      Show Abstract
      Chromatin interaction analysis using paired-end tag sequencing.
      Melissa J Fullwood,Yuyuan Han,Chia-Lin Wei,Xiaoan Ruan,Yijun Ruan
      Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.] Chapter 21 2010

      Show Abstract

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