Key Spec Table
|Species Reactivity||Key Applications||Host||Format||Antibody Type|
|R, H, M||WB, ICC, IHC, IH(P), IP||Rb||Culture Supernatant||Monoclonal Antibody|
|Safety Information according to GHS|
|Reference overview||Pub Med ID|
|Left ventricular hypertrophy is prevalent in Sprague-Dawley rats. |
Ryan M McAdams,Ronald J McPherson,Nazila M Dabestani,Christine A Gleason,Sandra E Juul
Comparative medicine 60 2010
Unrecognized cardiovascular abnormalities may confound the interpretation of research data collected using rats. However, although SPF rat colonies are screened for microbes and kept under standardized environmental conditions, their cardiovascular status is largely unknown. We recently performed surgery on anesthetized 80-d-old Sprague-Dawley rats and observed a high mortality that could not be attributed to the procedures or preceding treatments. Upon necropsy, cardiomyopathy was readily apparent in a substantial proportion of these rats. To further evaluate the nature of this condition, we evaluated the histology and morphology of hearts from both Sprague-Dawley and Lewis rats. Compared with Lewis rats, Sprague-Dawley rats had greater left ventricular wall thickness and larger cardiomyocyte cell size. Severe left ventricle hypertrophy was present in 38% of young adult Sprague-Dawley rats. These findings may have implications for research models that use Sprague-Dawley rats.Full Text Article
|Histone deacetylase 3 is required for centromeric H3K4 deacetylation and sister chromatid cohesion. |
Eot-Houllier, Grégory, et al.
Genes Dev., 22: 2639-44 (2008) 2008
We describe here the role of histone deacetylase 3 (HDAC3) in sister chromatid cohesion and the deacetylation of histone H3 Lys 4 (H3K4) at the centromere. HDAC3 knockdown induced spindle assembly checkpoint activation and sister chromatid dissociation. The depletion of Polo-like kinase 1 (Plk1) or Aurora B restored cohesion in HDAC3-depleted cells. HDAC3 was also required for Shugoshin localization at centromeres. Finally, we show that HDAC3 depletion results in the acetylation of centromeric H3K4, correlated with a loss of dimethylation at the same position. These findings provide a functional link between sister chromatid cohesion and the mitotic "histone code".
|Deletion of histone deacetylase 3 reveals critical roles in S phase progression and DNA damage control. |
Bhaskara, Srividya, et al.
Mol. Cell, 30: 61-72 (2008) 2008
|Dissecting HDAC3-mediated tumor progression. |
Mariadason, John M
Cancer Biol. Ther., 7: 1581-3 (2008) 2008