04-230 | Anti-HDAC3 Antibody, clone Y415, rabbit monoclonal

Immunofluorescent analysis of HeLa cells using anti-Histone Deacetylase 2 (C-term) antibody.
04-230
100 μL  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, MICC, WB, IP, IH(P), IHCRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number 04-230
      Brand Family Upstate
      Synonyms histone deacetylase 3
      Trade Name
      • Upstate
      Description Anti-HDAC3 Antibody, clone Y415, rabbit monoclonal
      Background Information Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. Histone Deacetylase 3 (HDAC3) belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. HDAC3 is regarded as a potential tumor suppressor gene that also plays critical roles in S phase progression and DNA damage control.
      References
      Product Information
      Format Culture Supernatant
      Specificity This antibody recognizes Histone Deacetylase 3 at the C-terminus.
      Control
      • K562 whole cell lysate.
      Presentation Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      Application Use Anti-HDAC3 Antibody, clone Y415 (Rabbit Monoclonal Antibody) validated in ICC, IHC, IHC(P), IP, WB to detect HDAC3 also known as histone deacetylase 3.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      • Immunoprecipitation
      • Immunohistochemistry (Paraffin)
      • Immunohistochemistry
      Application Notes Immunoprecipitation:
      1:50 dilution from a previous lot was used for immunoprecipitation.
      Biological Information
      Immunogen species KLH conjugated to a synthetic peptide from the C-terminal region of human Histone Deacetylase 3.
      Epitope C-terminus
      Clone Y415
      Host Rabbit
      Specificity This antibody recognizes Histone Deacetylase 3 at the C-terminus.
      Isotype IgG
      Species Reactivity Human Rat Mouse
      Species Reactivity Note Demonstrated to react with human, mouse and rat HDAC3.
      Antibody Type Monoclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events.
      Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq].
      Gene Symbol
      • EC 3.5.1.98
      • SMAP45
      • RPD3-2
      • RPD3
      • HD3
      Purification Method Unpurified
      UniProt Number
      UniProt Summary FUNCTION: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor.

      Catalytic activity Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone.

      SUBUNIT: Interacts with HDAC7 and HDAC9. Forms a heterologous complex at least with YY1. Interacts with DAXX, HDAC10 and DACH1. Found in a complex with NCOR1 and NCOR2. Component of the N-Cor repressor complex, at least composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1R, CORO2A and GPS2. Interacts with BCOR, MJD2A/JHDM3A, NRIP1 and SRY. Interacts with BTBD14B. Interacts with GLIS2 By similarity. Interacts with CBFA2T3.

      SUBCELLULAR LOCATION: Nucleus.

      TISSUE SPECIFICITY: Widely expressed.

      PTM: Sumoylated in vitro. Ref.14

      SIMILARITY: Belongs to the histone deacetylase family. Type 1 subfamily.

      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Routinely evaluated by Western Blot on K562 whole cell lysate.

      Western Blot Analysis:
      A 1:10,000 dilution of this lot was used to detect Histone Deacetylase 3 in K562 whole cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size 100 μL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Languages
      Deutschland

      Certificates of Analysis

      TitleLot Number
      Anti-HDAC 3, clone Y415 - 2115911 2115911
      Anti-HDAC 3, clone Y415 - 2426482 2426482
      Anti-HDAC 3, clone Y415 - 1977604 1977604
      Anti-HDAC 3, clone Y415 - 2075081 2075081
      Anti-HDAC 3, clone Y415 - 2274241 2274241
      Anti-HDAC 3, clone Y415 - NG1576341 NG1576341
      Anti-HDAC 3, clone Y415 - NG1746521 NG1746521
      Anti-HDAC 3, clone Y415 - NG1869262 NG1869262
      Anti-HDAC 3, clone Y415 - NG1880217 NG1880217
      Anti-HDAC 3, clone Y415 - NG1894302 NG1894302
      Anti-HDAC 3, clone Y415 - NG1908252 NG1908252
      Anti-HDAC 3, clone Y415 - NG1946513 NG1946513

      References

      Reference overviewPub Med ID
      Left ventricular hypertrophy is prevalent in Sprague-Dawley rats.
      Ryan M McAdams,Ronald J McPherson,Nazila M Dabestani,Christine A Gleason,Sandra E Juul
      Comparative medicine 60 2010

      Show Abstract Full Text Article
      21262120
      Dissecting HDAC3-mediated tumor progression.
      Mariadason, John M
      Cancer Biol. Ther., 7: 1581-3 (2008) 2008

      18787408
      Histone deacetylase 3 is required for centromeric H3K4 deacetylation and sister chromatid cohesion.
      Eot-Houllier, Grégory, et al.
      Genes Dev., 22: 2639-44 (2008) 2008

      Show Abstract
      18832068
      Deletion of histone deacetylase 3 reveals critical roles in S phase progression and DNA damage control.
      Bhaskara, Srividya, et al.
      Mol. Cell, 30: 61-72 (2008) 2008

      18406327