07-789 | Anti-phospho-AKT (Thr34) Antibody

Immunoblot Analysis Lysates from untreated (lane 1) and Calyculin A treated (100nM for 30 min.) (lane 2) A431 cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-phospho-AKT (Thr34) for 2 hours at room temperature. Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-AKT (Thr 34).
Immunoblot Analysis Lysates from untreated (lane 1) and Calyculin A treated (100nM for 30 min
07-789
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      Overview

      Replacement Information

      重要规格表

      品种反应性 主要应用 宿主 格式 抗体类型
      HWBRbAffinity PurifiedPolyclonal Antibody
      描述
      产品目录编号 07-789
      品牌系列 Upstate
      同义词 Akt1, PKB, PRKBA, RAC, RAC-ALPHA
      商名
      • Upstate
      描述 Anti-phospho-AKT (Thr34) Antibody
      参考
      产品信息
      格式 Affinity Purified
      演示 100 μL of epitope specific affinity purified rabbit polyclonal IgG in 50% affinity storage buffer (PBS containing 1.0 mg/mL BSA and 0.05% sodium azide) and 50% glycerol.
      应用
      应用 Anti-phospho-AKT (Thr34) Antibody detects level of phospho-AKT (Thr34) & has been published & validated for use in WB.
      主要应用
      • Western Blotting
      生物信息
      免疫原品种 KLH conjugated peptide corresponding to amino acid region encompassing the human phospho-AKT (Thr34); Sequence highly conserved in mouse and rat.
      宿主 Rabbit
      特异性 Recognizes phospho-AKT (Thr34)
      同种型 IgG
      品种反应性 Human
      Species Reactivity Note Predicted to cross react with Mouse and Rat due to high sequence homology, but have not been tested.
      抗体类型 Polyclonal Antibody
      Entrez基因编号
      Entrez基因汇总 The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
      基因符号
      • AKT
      • AKT1
      • RAC
      • PRKBA
      • MGC99656
      • RAC-ALPHA
      • RAC-PK-alpha
      • C-AKT
      • PKB
      修改
      • Phosphorylation
      纯化方法 Immunoaffinity purified
      UniProt编号
      UniProt汇总 FUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
      SIZE: 480 amino acids; 55686 Da
      SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).
      TISSUE SPECIFICITY: In all human cell types so far analyzed.
      DOMAIN: SwissProt: P31749 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
      PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).
      SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
      理化信息
      尺寸
      材料信息
      毒性学信息
      GHS安全信息
      安全信息
      产品使用声明
      质量保证 Routinely evaluated by immunoblot.
      使用声明
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      储存和货运信息
      存储条件 Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      包装信息
      数量 100 µL
      运输信息
      补充信息
      规格

      Documentation

      分析证书

      标题批号
      Anti-phospho-AKT (Thr34) (Affinity purified Rabbit polyclonal IgG) - 24425492442549
      Anti-phospho-AKT (Thr34) (Affinity purified Rabbit polyclonal IgG) - DAM1467540DAM1467540
      Anti-phospho-AKT (Thr34) - 06030262410603026241

      参考

      参考概述应用公共医疗ID
      Progesterone receptor enhances breast cancer cell motility and invasion via extranuclear activation of focal adhesion kinase.
      Fu XD, Goglia L, Sanchez AM, Flamini M, Giretti MS, Tosi V, Genazzani AR, Simoncini T
      Endocr Relat Cancer 17 431-43. Print 2010. 2010

      显示摘要
      20233709
      Extra-nuclear signaling of progesterone receptor to breast cancer cell movement and invasion through the actin cytoskeleton.
      Xiao-Dong Fu, Maria S Giretti, Chiara Baldacci, Silvia Garibaldi, Marina Flamini, Angel Matias Sanchez, Angiolo Gadducci, Andrea R Genazzani, Tommaso Simoncini
      PloS one 3 e2790 2008

      显示摘要 全文本文章
      Immunoblotting (Western)18665217
      Cisplatin activates Akt in small cell lung cancer cells and attenuates apoptosis by survivin upregulation
      Belyanskaya, Larisa L, et al
      Int J Cancer, 117:755-63 (2005) 2005

      15981204
      Ceramide disables 3-phosphoinositide binding to the pleckstrin homology domain of protein kinase B (PKB)/Akt by a PKCzeta-dependent mechanism
      Powell, Darren J, et al
      Mol Cell Biol, 23:7794-808 (2003) 2003

      14560023
      PKB/Akt: connecting phosphoinositide 3-kinase to cell survival and beyond.
      Marte, B M and Downward, J
      Trends Biochem. Sci., 22: 355-8 (1997) 1997

      显示摘要
      9301337