Isopore™ Membrane Filters

Polycarbonate membrane for microscopy and visual analysis



Guide d'achat

White Membrane Filters · Catalogue No. Supprimer le tri & les filtres
Référenceicon Dimension de pores (µm)icon Diamètre du filtre (⌀)icon Conditionnementicon
ATTP013000,813 mm100 Prix & Disponibilité
ATTP025000,825 mm100 Prix & Disponibilité
ATTP037000,837 mm100 Prix & Disponibilité
ATTP047000,847 mm100 Prix & Disponibilité
ATTP142500,8142 mm50 Prix & Disponibilité
DTTP013000,613 mm100 Prix & Disponibilité
DTTP025000,625 mm100 Prix & Disponibilité
DTTP047000,647 mm100 Prix & Disponibilité
GTTP013000,213 mm100 Prix & Disponibilité
GTTP025000,225 mm100 Prix & Disponibilité
GTTP037000,237 mm100 Prix & Disponibilité
GTTP047000,247 mm100 Prix & Disponibilité
GTTP090300,290 mm30 Prix & Disponibilité
GTTP142500,2142 mm50 Prix & Disponibilité
HTTP013000,413 mm100 Prix & Disponibilité
HTTP025000,425 mm100 Prix & Disponibilité
HTTP037000,437 mm100 Prix & Disponibilité
HTTP047000,447 mm100 Prix & Disponibilité
HTTP090300,490 mm30 Prix & Disponibilité
HTTP142500,4142 mm50 Prix & Disponibilité
RTTP013001,213 mm100 Prix & Disponibilité
RTTP025001,225 mm100 Prix & Disponibilité
RTTP047001,247 mm100 Prix & Disponibilité
RTTP142501,2142 mm50 Prix & Disponibilité
TCTP0130010,013 mm100 Prix & Disponibilité
TCTP0250010,025 mm100 Prix & Disponibilité
TCTP0470010,047 mm100 Prix & Disponibilité
TCTP1425010,0142 mm50 Prix & Disponibilité
TETP013008,013 mm100 Prix & Disponibilité
TETP025008,025 mm100 Prix & Disponibilité
TETP047008,047 mm100 Prix & Disponibilité
TETP090308,090 mm30 Prix & Disponibilité
TETP142508,0142 mm50 Prix & Disponibilité
TMTP013005,013 mm100 Prix & Disponibilité
TMTP025005,025 mm100 Prix & Disponibilité
TMTP047005,047 mm100 Prix & Disponibilité
TMTP090305,090 mm30 Prix & Disponibilité
TMTP142505,0142 mm50 Prix & Disponibilité
TSTP013003,013 mm100 Prix & Disponibilité
TSTP025003,025 mm100 Prix & Disponibilité
TSTP047003,047 mm100 Prix & Disponibilité
TSTP142503,0142 mm50 Prix & Disponibilité
TTTP025002,025 mm100 Prix & Disponibilité
TTTP047002,047 mm100 Prix & Disponibilité
VCTP013000,113 mm100 Prix & Disponibilité
VCTP025000,125 mm100 Prix & Disponibilité
VCTP047000,147 mm100 Prix & Disponibilité
VCTP142500,1142 mm50 Prix & Disponibilité

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Brown Membrane Filters Supprimer le tri & les filtres
Référenceicon Dimension de pores (µm)icon Diamètre du filtre (⌀)icon Conditionnementicon
GTBP013000,213 mm100 Prix & Disponibilité
GTBP025000,225 mm100 Prix & Disponibilité
GTBP047000,247 mm100 Prix & Disponibilité
HTBP013000,413 mm100 Prix & Disponibilité
HTBP025000,425 mm100 Prix & Disponibilité
HTBP047000,447 mm100 Prix & Disponibilité

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    Références bibliographiques

    Aperçu de la référence bibliographique
    Urea transport in kidney brush-border membrane vesicles from an elasmobranch, Raja erinacea
    Robyn L. et al,J. experimental biology, 206, 3293-3302, 2003; internal ref BB04
    J. experimental biology, 206, 3293-3302, 2003; internal ref BB04 2003

    Comparison of microporous membrane morphologies using confocal scanning laser microscopy
    Charcosset C (reprint), et al.
    Journal of membrane science. 2000. v168, n2, p53-62 2000


    How do I clear my Millipore filters? It is possible to clear Millipore filters by choosing a clearing agent with the same refractive index as the membrane. This is called the PORE FILLING TECHNIQUE. Filters appear opaque because of the diffraction of light through the tortuous pores. Filling the pores with a liquid that has the same refractive index as the membrane (ex. xylene for MF filters) allows light to pass through the filter at a uniform speed thus rendering the filter transparent. This technique can be used for most membrane filters with a single refractive index. The refractive index for Durapore membrane is 1.42 and for MF membrane its 1.50.

    Please note that this does not work for polycarbonate membranes because these membranes have more than one refractive index (1.62 and 1.58). If desired, the porous structure can be dissolved from Isopore polycarbonates by dissolving the filter in chloroform, methylene chloride or 1-methyl-2 pyrrolidone.
    What is the blue separation paper between my filters? It is a parchmentalized paper specially formulated for Millipore Corporation.
    How do I tell the membrane from the separator? The blue paper is the separator.
    Which side of the membrane should I use, the shiny or dull side? Most researchers may not even notice that there is a "sidedness" to filters, and, for most applications, orientation will not affect filter performance. However, membranes do have a slightly asymmetric pore structure: the shiny side of the membrane is the "tighter" side. In some applications, you can take advantage of this difference by selecting a specific filter orientation. ultrafiltration membranes should always be used shiny side up, regardless of application for drop dialysis ( a buffer exchange technique in which a few drops of DNA or protein are placed in a 0.05 or 0.025 um filter and floated on a buffer solution), apply the sample to the shiny side of the filter and float the filter dull side to the buffer. This measure will enhance buffer exchange and discourage sample loss. The Millipore Express and Express Plus membranees are also sided - these membranes should be used shiny side facing down.
    What is the difference between pore size and pore size distribution? Whereas pore size is a measure of the diameter of the largest pore, pore size distribution is a measure of the range of pore sizes. The range of pore sizes can be normally distributed, and the spread can be quite narrow (e.g. the ratio of largest to smallest may be less than 2). On the other hand, pore size distribution can be very heterogeneous. In the case of large spreads and heterogeneity, the pore size will be far less predictive of flow rate (either filtration or capillary) than it will be for a membrane with a narrow pore size distribution. It is important to note that the pore size corresponding to the bubble point is not at the middle of the distribution, but is the largest pore.
    Is Polyvinylpyrolidone (PVP) toxic to cell growth on our Isopore membrane? PVP coated membranes are not suitable and will not support the attachment of cells to the membrane. PVP in Millicell-PC membranes has been validated for hybridoma cell growth and subsequent antibody production and found to be non-toxic. PVP is generally considered non-toxic and for a while was used in vivo as a plasma expander.