48-671MAG | MILLIPLEX MAP Total Mitogenesis RTK Magnetic Bead 7-Plex Kit - Cell Signaling Multiplex Assay

Multiplex analysis of HEK293 cells treated with serum.
48-671MAG
96-well plate  96-well plate
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      Overview

      Replacement Information

      Tableau de caractéristiques principal

      Analytes disponibles Espèces réactives Principales applications Méthodes de détection
      IGF-1R (Total), EGFR (Total), ErbB2/HER2 (Total), ErbB3/HER3 (Total), ErbB4/HER4 (Total), c-Met/HGFR (Total)HumainMplexLuminex xMAP
      Description
      Référence 48-671MAG
      Nom de marque
      • MILLIPLEX
      Description MILLIPLEX MAP Total Mitogenesis RTK Magnetic Bead 7-Plex Kit - Cell Signaling Multiplex Assay
      Aperçu RTKs (Receptor Tyrosine Kinases) are transmembrane proteins that act as receptors for growth factors, neurotrophic factors and other extracellular signaling molecules. Upon binding extracellular ligands, RTKs can undergo receptor dimerization and tyrosine phosphorylation on specific residues in the cytoplasmic tails which then lead to RTK-mediated signal transduction. Of the greater than 500 known protein kinases in the human genome, approximately 60 are members of the RTK family. They are initiators of cell signaling networks and play crucial roles in normal physiological processes and disease processes ranging from diabetes to cancer.
      Références bibliographiques
      Informations produit
      Méthode de détection Luminex xMAP
      Configuration Premixés
      Précision (%)
      • Inter-Assay: < 9
      • Intra-Assay: < 11
      Type de panel Cell Signaling
      Applications
      Application The analytes available for this multiplex kit are: EGFR (Total), ErbB2/HER2 (Total), ErbB3/HER3 (Total), ErbB4/HER4 (Total), IR (total), c-Met/HGFR (Total), IGF-1R (Total).
      Principales applications
      • Multiplexing
      Notes opératoires • An overnight (4°C) incubation is recommended for best results.
      • This assay requires 25 µL diluted cell lysate per well.
      • This kit must be run using Assay Buffer 1 (provided).
      • 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL).
      Informations biologiques
      Spécificité Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Espèces réactives Humain
      Analytes disponibles
      • IGF-1R (Total)
      • EGFR (Total)
      • ErbB2/HER2 (Total)
      • ErbB3/HER3 (Total)
      • ErbB4/HER4 (Total)
      • c-Met/HGFR (Total)
      Informations physico-chimiques
      Dimensions
      Informations sur les matériaux
      Informations toxicologiques
      Informations de sécurité selon le SGH
      Informations sur la sécurité
      Notifications sur l'utilisation du produit
      Notification sur l'utilisation
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Informations relatives au stockage et à l'expédition
      Conditions de stockage Recommended storage for kit components is 2 - 8°C.
      Informations sur l'emballage
      Quantité 96-well plate
      Conditionnement 96-well plate
      Informations sur le transport
      Information complémentaire
      Caractéristiques

      Documentation

      Protocols

      Title
      7-plex RTK Mitogenesis Total Protein Magnetic Bead Kit

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      Brochure

      Titre
      Advancing cancer research: From hallmarks & biomarkers to tumor microenvironment progression
      Immunoassay & Multiplex Kits: Analyte Quarterly, Volume 1, 2015
      The Power of Biomarker Analysis

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      Full Service Custom-built Assays

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      Cancer & Metastasis Signaling Pathways