FTLV00003 | Fast-Trap Lentivirus Purification and Concentration Kit

FTLV00003
3 purifications and concentrations  3 purifications and concentrations
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      Overview

      Replacement Information
      Descripción
      Número de catálogo FTLV00003
      Nombre comercial
      • Fast-Trap
      Descripción Fast-Trap Lentivirus Purification and Concentration Kit
      Visión de conjunto The Fast-Trap Lentivirus Purification and Concentration Kit contains the reagents, filtration devices, and concentration devices necessary to purify the virus away from cellular contaminants and the expressed recombinant transgene. It yields concentrated virus in the exchange buffer of choice, suitable for in vitro and animal studies. The kit provides a quick, easy, membrane-based method for the laboratory scale purification of ≥ 3 mL of lentivirus, up to capacity of approximately 2x108 total infectious lentivirus particles.

      The purification kit protocol calls for collecting the supernatant from transfected cells that have released the virus. Most of the large cellular debris is removed by centrifugation, leaving the viable virus particles in the supernatant. The supernatant is further clarified by passing it through a Steriflip-HV 0.45 μm filter. After adding a binding buffer, the virus solution is passed over a treated filter that adsorbs the virus particles, allowing the smaller cellular debris and media to pass through the filter. Following a wash to remove any weakly bound proteins, the virus is eluted off the filter with an elution buffer. Finally, the virus can be concentrated and exchanged into the desired buffer using an Amicon® Ultra centrifugal filter unit.

      For laboratory use only; not for diagnostic or in vivo use.
      Referencias bibliográficas
      Información del producto
      Componentes
      • 3 – Fast-Trap virus filter units
        3 – Steriflip-HV filter units
        1 – 10x Binding buffer, 20 mL
        1 – Equilibration buffer, 100 mL
        1 – Wash buffer, 200 mL
        1 – Elution buffer, 21 mL
        3 – Amicon Ultra-4 filter units, 100 kDa NMWL
        1 – Test tube stand
        1 – User Guide
        1 – Overview of Procedure card
        1 – Material Safety Data Sheet
      Aplicaciones
      Aplicaciones clave
      • Purificación y concentración de lentivirus
      Información biológica
      Información fisicoquímica
      Dimensiones
      Información de materiales
      Información toxicológica
      Información de seguridad según el GHS
      Información de seguridad
      Declaraciones de uso del producto
      Declaración de uso
      • General Lab Use
      Información de almacenamiento y transporte
      Condiciones de almacenamiento The Fast-Trap Lentivirus Purification and Concentration Kit should be stored at room temperature.

      Warnings and Precautions:

      WARNING: This kit permits quick purification of lentivirus, an infectious agent which should be handled under BSL2 safety precautions. Wear hand, eye, face and body personal protective equipment (PPE) when using this kit. Millipore Corporation takes no responsibility for improper use of this kit.

      WARNING:All virus purification steps (except water bath incubation and centrifugation) should be conducted in a biosafety cabinet.
      For research use only. Not for use in diagnostic or human clinical procedures.
      Variation in the amount of lentivirus purified with this kit may be due in part to infection success and cell conditions.
      Información sobre embalaje
      Cantitad 3 purifications and concentrations
      Envase 3 purifications and concentrations
      Información de transporte
      Información complementaria
      Especificaciones

      Documentation

      Ficha datos de seguridad (MSDS)

      Idiomas
      English

      Licencias necesarias e Información técnica

      Cargo
      Improved Method for Adenovirus and Lentivirus Purification using the Fast-Trap™ Virus Purification and Concentration Kit

      Ficha técnica

      Cargo
      Fast-Trap™- Lentivirus and Adenovirus Purification and Concentration Kits

      Póster

      Cargo
      A New Vacuum-Based Method for Adenovirus Purification and Concentration
      New Workflow for Lentivirus Purification, Concentration, and Immunodetection,
      Poster: Improved Method For Adeno and Lenti- Virus Purification and Titration
      Poster: Improved Method for Adeno and Lenti-Virus Purification and Titration

      Manuales del usuario

      Cargo
      Fast-Trap Lentivirus Purification and Concentration Kit
      Fast-Trap Lentivirus Purification and Concentration Kit

      Productos y aplicaciones relacionados

      Familias de productos

      Productos relacionados por: Brand Facette

      Categorías

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Mammalian Expression > Lentivirus and Adenovirus Related Products