Merck Millipore Webinars

Upcoming and On-Demand


View upcoming and on-demand webinars to learn advanced research techniques, valuable tips and advice from leaders in their fields of scientific research.

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Upcoming Webinars

Take part in our complimentary, live webinars, where you’ll interact with experts who are breaking ground in the industrial microbiology, life science research, IVD/OEM, pharmaceutical and biopharmaceautical industries. You’ll learn about the newest tools and technologies while gathering valuable tips and troubleshooting strategies for the applications most relevant to your processes.

Find an upcoming webinar below and register today!


Biopharmaceutical Manufacturing and Small Molecule Pharmaceuticals

IEX Chromatography: How to Get the Most out of Your mAb Process

  • Presenters: Prof. Dr. Christian Frech, Head of the Institute for Biochemistry, Mannheim University of Applied Sciences, Germany and Dr. Lothar Jacob, Product Manager for Ion Exchange Chromatography, Process Solutions, Merck Millipore
  • Date: Thursday, May 28, 2015
  • Duration: 1 Hour
  • Session 1: 9:00 AM CET, 3:00 AM EST
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  • Session 2: 5:00 PM CET, 11:00 AM EST
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Abstract:
During the downstream processing of monoclonal antibodies (mAbs), ion exchange chromatography (IEX) is a very crucial purification tool. It is mostly utilized as post-protein A and/or polishing step.

Within both types of ion exchange chromatography - cation exchange (CEX) and anion exchange (AEX) - the optimization of operating parameters can enhance the entire purification scheme. Antibody aggregate removal can be significantly improved through CEX if the monomeric forms elutes before the aggregates come off the column. Choosing the right elution condition is key for efficiently separating host cell proteins (HCPs) as well. The main contribution of anion exchange (AEX) chromatography is efficient DNA and virus removal, in many applications in a flow-through mode. However, also CEX can contribute to additional process safety with regard to virus removal even in bind-elute mode. Key factors influencing the separation of mAbs on ion exchange chromatographic columns as well as case studies will be presented during this webinar.


Managing Implementation and Regulatory Risks: Single Use Technologies for Final Fill

  • Presenters: Jessica Shea, E&L Global Technical Services Manager, Provantage® Lab Services, Merck Millipore and Sue Walker, Market Development Manager, Final Fill, Merck Millipore
  • Date: Thursday, June 11, 2015
  • Duration: 1 Hour
  • Session: 5:00 PM CET, 11:00 AM EST
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Abstract:
The benefits of single use technologies are well documented. Risks associated with product loss, sterility and patient safety are magnified in the filling operation because of its closeness to the final product form and the patient. A systematic and thorough evaluation of the assembly manufacturing and filling process will identify risks that may be managed by the supplier and end user working together to create sound initial designs that are fit for the intended use. Managing regulatory and patient safety risks requires an accurate interpretation of regulatory requirements and the design of a step wise approach to identify and mitigate potentially toxic compounds that may leach from process components. This webinar will include remarks on key aspects of assembly manufacture and quality control; extractables and leachables, patient safety and implementation by the end user.

Life Science Research

Extracellular Vesicle Enrichment and Characterization from Cell Culture Supernatants and Biological Fluids

  • Presenters: Dr. Aled Clayton, Exosome Biology Group, Cardiff University and Dr. Amedeo Cappione III, Senior Scientist, Merck Millipore
  • Date: Thursday, May 21, 2015
  • Time: 11am-12pm Eastern (8am-9am Pacific; 4pm-5pm BST; 5pm-6pm CEST)
  • Duration: 1 hour

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Abstract:
There has been controversy about the biological relevance of exosomes and other extracellular vesicles (EVs), but there is now substantial evidence pointing to EVs as cell-to-cell communication devices with key roles in a variety of disease settings. Although such evidence continues to grow each year, the study of EVs remains challenging. There are several approaches for concentrating vesicles generated in vitro or those present in biological fluids, including ultracentrifugation combined with a density gradient, chromatography and precipitation-based approaches. The tools used to analyze the specimen are also diverse, including particle analyzers, array methods for phenotyping the specimen quickly, and traditional western blotting and electron microscopy tools. The challenge is to obtain a sufficient quantity of vesicles of defined purity for downstream analyses or functional assessment. In this webinar, Dr. Aled Clayton (Cardiff University) will discuss some of the most common methods for preparing and characterizing EVs, along with their particular advantages and challenges. In addition, Dr. Clayton will discuss The International Society for Extracellular Vesicles’ recommended best practices with respect to these issues. Dr. Amedeo Cappione, III (Merck Millipore) will then describe a recently developed protocol for enriching EV samples using centrifugal ultrafiltration, monitoring enrichment by IR spectrometry and rapid western blot analysis, followed by relative quantitation using flow cytometry. The ultimate choice of approach, however, should be driven by the research question/application, and these aspects will also be discussed.


Editors’ Series: Hi-Tech, Low-Tech and No-Tech: Preparing Samples for LC and LC-MS

  • Presenter: Nicholas H. Snow, Department of Chemistry and Biochemistry, Center for Academic Industry Partnership, Seton Hall University
  • Date: Thursday, May 28, 2015
  • Time: 1:00 PM Eastern Daylight Time
  • Duration: 1 hour

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Abstract:
Sample preparation for chromatographic analysis of small molecules such as drugs and pesticides has evolved significantly over the past decade, becoming faster, more automated, and capable of extracting analytes for analysis at part-per-trillion and lower levels. This presentation will compare and contrast three approaches that have evolved most significantly. Solid-phase extraction is a classical technique that has received a recent high-tech boost from automation and miniaturization. Over the past decade, the QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) approach provided a simple, low-tech alternative that has greatly impacted the analysis of pesticides and residues from numerous matrices, especially foods. Finally, multidimensional detection has led to a no-tech sample preparation approach in LC-MS analysis: none. Especially in multi-residue drug analysis from biological samples, methods involving no sample preparation at all are generating interest. The choice of approach depends on several chemical variables, including sample type, analytes, and desired analytical performance, plus managerial variables including cost, throughput and simplicity. Using examples drawn from the analysis of drug and pesticide residues in biological samples, these three approaches will be compared and contrasted with a view toward assisting laboratories in choosing or improving their own methods and techniques for these often difficult analyses.


Overcoming Sample-related Challenges for Elispot and Other Functional T cell Assays To Produce More Reliable Immune Monitoring

  • Presenter: Dr. Sylvia Janetzki, Founder and President, ZellNet Consulting
  • Date: Wednesday, June 10, 2015
  • Time: 10am-11pm Eastern (7am-8am Pacific; 3pm-4pm BST; 4pm-5pm CEST)
  • Duration: 1 hour

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Abstract:
Immune monitoring is an integral part of immunological research and translational applications in the fields of cancer, infectious disease, autoimmunity, transplantation and others. The functional assessment of immune cells heavily relies on the availability of adequate amounts of intact samples. In this live webinar with Q&A, you’ll hear Elispot expert Dr. Sylvia Janetzki (ZellNet Consulting) describe the latest improvements made to strategies for obtaining and handling patient samples to conserve sample integrity. Various specific topics will be discussed, including obtaining blood and tissue samples; processing, storage and transport of samples; handling of samples in multi-center trials; and tools to preserve stability and functionality of samples including re-setting circulating T cell functionality to a tissue-like state.