Key Spec Table
|Safety Information according to GHS|
|Product Usage Statements|
|Storage and Shipping Information|
Mortar, pestle, and cell strainers can be stored at room temperature.
Single use vials containing the enzymes Dispase II and Collagenase I should be stored at 2° to 8°C until ready to use.
|Material Size||10 isolations/kit|
|Reference overview||Pub Med ID|
|Granulocyte colony stimulating factor expands hematopoietic stem cells within the central but not endosteal bone marrow region. |
Jochen Grassinger,Brenda Williams,Gemma H Olsen,David N Haylock,Susan K Nilsson
Cytokine 58 2012
Granulocyte colony stimulating factor (G-CSF) is clinically well established for the mobilization of hematopoietic stem cells (HSC). Extensive data on the underlying mechanism of G-CSF induced mobilization is available; however, little is known regarding the functional effect of G-CSF on HSC within the bone marrow (BM). In this study we analyzed the proportion and number of murine HSC in the endosteal and central bone marrow regions after 4 days of G-CSF administration. We demonstrate that the number of HSC, defined as CD150(+)CD48(-)LSK cells (LSKSLAM cells), increased within the central BM region in response to G-CSF, but not within the endosteal BM region. In addition the level of CD150 and CD48 expression also increased on cells isolated from both regions. We further showed that G-CSF mobilized proportionally fewer LSKSLAM compared to LSK cells, mobilized LSKSLAM had colony forming potential and the presence of these cells can be used as a measure for mobilization efficiency. Together we provide evidence that HSC in the BM respond differently to G-CSF and this is dependent on their location. These findings will be valuable in developing new agents which specifically mobilize HSC from the endosteal BM region, which we have previously demonstrated to have significantly greater hematopoietic potential compared to their phenotypically identical counterparts located in other regions of the BM.
|Phenotypically identical hemopoietic stem cells isolated from different regions of bone marrow have different biologic potential. |
Jochen Grassinger,David N Haylock,Brenda Williams,Gemma H Olsen,Susan K Nilsson
Blood 116 2010
Hemopoietic stem cells (HSCs) reside within a specified area of the bone marrow (BM) cavity called a niche that modulates HSC quiescence, proliferation, differentiation, and migration. Our previous studies have identified the endosteal BM region as the site for the HSC niche and demonstrated that hemopoietic stem and progenitor populations (HSPCs, LSK) isolated from different BM regions exhibit significantly different hemopoietic potential. In this study, we have analyzed subpopulations of LSK cells isolated from different regions of the BM and showed that CD150(+)CD48(-)LSK HSCs within the endosteal BM region have superior proliferative capacity and homing efficiency compared with CD150(+)CD48(-)LSK HSCs isolated from the central BM. Furthermore, we show, for the first time, that a subset of CD150(+)CD48(+)LSK progenitor cells, previously defined as B-lymphoid primed hemopoietic cells, are capable of multilineage reconstitution, however, only when isolated from the endosteal region. In addition, we provide evidence for an unrecognized role of CD48 in HSC homing. Together, our data provide strong evidence that highly purified HSCs show functional differences depending on their origin within the BM and that the most primitive HSCs reside within the endosteal BM region.
|Glucagon supports postabsorptive plasma glucose concentrations in humans with biologically optimal insulin levels. |
Cooperberg BA, Cryer PE
Diabetes 59 2941-4. Epub 2010 Aug 10. 2010
OBJECTIVE: Based on the premise that postabsorptive patients with type 1 diabetes receiving intravenous insulin in a dose that maintains stable euglycemia are receiving biologically optimal insulin replacement, we tested the hypothesis that glucagon supports postabsorptive plasma glucose concentrations in humans.Full Text Article
|Investigating the interactions between haemopoietic stem cells and their niche: methods for the analysis of stem cell homing and distribution within the marrow following transplantation. |
Brenda Williams,Susan K Nilsson
Methods in molecular biology (Clifton, N.J.) 482 2009
Interactions between haemopoietic stem cells (HSC) and their microenvironment serve multiple functions including the attraction to and retention and regulation in the bone marrow HSC niche. However, the cell adhesion molecules involved, their HSC receptors and the mechanisms underpinning these processes remain poorly understood. An ability to thoroughly investigate the roles of specific molecules in this process relies on a variety of in vitro and in vivo assays including the assessment of a HSC ability to home to the bone marrow and analysis of its lodgement within the bone marrow.
|Cellutions Newsletter: 2007, Volume 1|
|Cellutions Newsletter: 2008, Volume 1|
|Cellutions Newsletter: 2008, Volume 2|
|Cellutions Newsletter: 2008, Volume 3|
|Maximal Recovery of Hematopoietic Stem Cells from Bone Marrow Endosteum|
|Bone Marrow Harvesting & Hematopoietic Stem Cell Isolation Kit|