MAB2137 | Anti-Homing Receptor Antibody, clone A020

MAB2137
100 µg  
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      Overview

      Replacement Information

      重要规格表

      品种反应性 主要应用 宿主 格式 抗体类型
      Rb, B, Ca, H, M, Po, RWB, ELISA, FC, ICC, IHCRPurifiedMonoclonal Antibody
      描述
      产品目录编号 MAB2137
      品牌系列 Chemicon®
      同义词 CD44s; HCAM; Pgp-1
      商名
      • Chemicon
      描述 Anti-Homing Receptor Antibody, clone A020
      参考
      产品信息
      格式 Purified
      特异性 Antibody reacts with the standard CD44 isoform (CD44s) that lacks exon sequences v1-v10. Also called human CD44 (85-95 kDa) gp90{Hermes} and mouse gp85 (Pgp-1).
      控制
      • Cells of epithelium and highly expressed by carcinomas
      演示 Liquid in 10 mM sodium phosphate, pH 7.5 with 150 mM NaCl and 0.01% sodium azide.
      应用
      应用 Anti-Homing Receptor Antibody, clone A020 is an antibody against Homing Receptor for use in ELISA, FC, IC, IH & WB.
      主要应用
      • Western Blotting
      • ELISA
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry
      应用说明 ELISA 1:2000

      Flow Cytometry :1:1000

      Immunofluorescence 1:200

      Immunohistostaining of paraffin embedded tissues. 1:250; antigen retrieval is usually not needed.

      Immunoprecipitation 1:200

      Western blotting: non-reduced conditions only; 90kDa band is detected; blocking PBS/5% milk protein, primary antibody dilution 1:1000 o/n incubation.

      Optimal working dilutions must be determined by end user.
      生物信息
      免疫原品种 Purified human blood lymphocyte CD44
      克隆 A020
      浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
      宿主 Rat
      特异性 Antibody reacts with the standard CD44 isoform (CD44s) that lacks exon sequences v1-v10. Also called human CD44 (85-95 kDa) gp90{Hermes} and mouse gp85 (Pgp-1).
      同种型 IgG2b
      品种反应性 Rabbit Bovine Canine Human Mouse Pig Rat
      抗体类型 Monoclonal Antibody
      Entrez基因编号
      Entrez基因汇总 The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
      基因符号
      • HCELL
      • CD44
      • LHR
      • HUTCH-I
      • CDW44
      • CDw44
      • ECMR-III
      • Epican
      • MGC10468
      • CD44R
      • PGP-1
      • MDU2
      • CSPG8
      • Pgp1
      • IN
      • MUTCH-I
      • MIC4
      • MDU3
      • MC56
      纯化方法 Protein A purfied
      UniProt编号
      UniProt汇总 FUNCTION: SwissProt: P16070 # Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
      SIZE: 742 amino acids; 81554 Da
      SUBUNIT: Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: An epithelial isoform (CD44E) is expressed by cells of epithelium and highly expressed by carcinomas. An hematopoietic isoform (CD44H) is expressed by cells of mesodermal origin. Expression is repressed in neuroblastoma cells.
      PTM: Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors. & N-glycosylated. & O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). & Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
      SIMILARITY: SwissProt: P16070 ## Contains 1 Link domain.
      理化信息
      尺寸
      材料信息
      毒性学信息
      GHS安全信息
      安全信息
      产品使用声明
      使用声明
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      储存和货运信息
      存储条件 Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      包装信息
      数量 100 µg
      运输信息
      补充信息
      规格

      Documentation

      MSDS

      语言
      English

      分析证书

      标题批号
      RAT ANTI-CD44S MONOCLONAL ANTIBODY - 21470282147028
      RAT ANTI-CD44S MONOCLONAL ANTIBODY - 23988662398866
      RAT ANTI-CD44S -25670962567096
      RAT ANTI-CD44S MONOCLONAL ANTIBODY - 22956332295633

      参考

      参考概述应用品种公共医疗ID
      Peripheral facial nerve axotomy in mice causes sprouting of motor axons into perineuronal central white matter: time course and molecular characterization.
      Milan Makwana,Alexander Werner,Alejandro Acosta-Saltos,Roman Gonitel,Abirami Pararajasingham,Crystal Ruff,Prakasham Rumajogee,Dan Cuthill,Mathias Galiano,Marion Bohatschek,Adam S Wallace,Patrick N Anderson,Ulrike Mayer,Axel Behrens,Gennadij Raivich
      The Journal of comparative neurology 518 2010

      显示摘要
      20034058
      Mature human thymocytes migrate on laminin-5 with activation of metalloproteinase-14 and cleavage of CD44.
      Vivinus-Nebot, Mylène, et al.
      J. Immunol., 172: 1397-406 (2004) 2004

      显示摘要
      Immunoblotting (Western)Human14734715
      Membrane-type 1 matrix metalloproteinase cleaves CD44 and promotes cell migration
      Kajita, M, et al.
      J Cell Biol, 153:893-904 (2001) 2001

      ImmunocytochemistryHuman11381077
      Correlation of CD44S expression in renal clear cell carcinomas with subsequent tumor progression or recurrence.
      M Z Gilcrease, M Guzman-Paz, G Niehans, D Cherwitz, J B McCarthy, J Albores-Saavedra, M Z Gilcrease, M Guzman-Paz, G Niehans, D Cherwitz, J B McCarthy, J Albores-Saavedra
      Cancer 86 2320-6 1999

      显示摘要
      10590373
      Hyaluronic acid-induced lymphocyte signal transduction and HA receptor (GP85/CD44)-cytoskeleton interaction.
      Bourguignon, L Y, et al.
      J. Immunol., 151: 6634-44 (1993) 1993

      显示摘要
      7505012

      小册子

      标题
      Human Stem Cell Systems

      数据表

      标题
      Anti-Homing Receptor, clone A020 - Data Sheet