MAB1281 | Anti-Nuclei Antibody, clone 235-1

Mouse anti-Human Nuclei (Catalogue No. MAB1281) staining of human neural stem cells transplanted into rat brain. Photo courtesy of ReNeuron.
Mouse anti-Human Nuclei (Catalogue No. MAB1281) staining of human neural stem cells transplanted int
MAB1281
100 µL  
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      Overview

      Replacement Information

      重要规格表

      品种反应性 主要应用 宿主 格式 抗体类型
      Human OnlyICCMPurifiedMonoclonal Antibody
      描述
      产品目录编号 MAB1281
      品牌系列 Chemicon®
      同义词 HuNu
      商名
      • Chemicon
      描述 Anti-Nuclei Antibody, clone 235-1
      背景信息 Cell replacement techniques such as stem-cell or fetal-cell therapies show great promise for many diseases. Cross-species grafting between human and animal cells or tissues requires the identification and localization of the donor material in the host environment. Antibodies to human-specific nuclei are useful cytological and histological markers for identifying human cells in xenograft models.
      参考
      产品信息
      格式 Purified
      控制
      • All human cell types
      演示 Hybridoma supernatant. Liquid in PBS. Contains no preservative.
      应用
      应用 Detect Nuclei using this Anti-Nuclei Antibody, clone 235-1 validated for use in IH(P) & IP.
      主要应用
      • Immunocytochemistry
      应用说明 Immunohistochemistry/cytochemistry: 4% PFA fixed cryosections, or 5-10 minuted fixed cell cultures. Acetone fixation is also effective. Permeabilization with 0.1-0.4% triton X-100 is suggested in the blocking buffer. Dilution will vary slightly with lot; use at 1:10-1:500.

      Formalin fixed, paraffin embedded tissues: 1:20 for 1hr at RT. Citrate buffer epitope retrieval method recommended.
      http://www.ihcworld.com/_protocols/antibody_protocols/nuclei_chemicon.htm

      Identifies an 82 and 70 kD protein by immunoprecipitation.

      Optimal working dilutions and protocols must be determined by the end user.
      生物信息
      克隆 235-1
      浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
      宿主 Mouse
      特异性 Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. Also stains nuclei from primate (Sanchez-Pernaute, 2005). The antibody does not react with nuclei from mouse or rat.
      同种型 IgG1
      品种反应性 Human Only
      Species Reactivity Note The human nuclei monoclonal reacts specifically with human cells. It shows no reactivity against mouse, rat, chicken in immunohistorchemistry. Other mammalian or animal species have not been tested for reactivity.
      抗体类型 Monoclonal Antibody
      纯化方法 Protein A purfied
      理化信息
      尺寸
      材料信息
      毒性学信息
      GHS安全信息
      安全信息
      产品使用声明
      使用声明
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      储存和货运信息
      存储条件 Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      包装信息
      数量 100 µL
      运输信息
      补充信息
      规格

      Documentation

      MSDS

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      分析证书

      标题批号
      MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 21277682127768
      MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 23665212366521
      MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 24531442453144
      MOUSE ANTI-HUMAN NUCLEI - 24921002492100
      MOUSE ANTI-HUMAN NUCLEI -25523402552340
      MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 19690981969098
      MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 20003722000372
      MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 20554362055436
      MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 21019642101964
      MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 21995612199561
      MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 22395932239593
      MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 22871542287154
      MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - JC1682922JC1682922
      MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - LV1514200LV1514200

      参考 | 70 有货 | 选择所有参考

      参考概述应用公共医疗ID
      Effect of Intermittent PTH(1-34) on Human Periodontal Ligament Cells Transplanted into Immunocompromised Mice.
      Michael Wolf,Stefan Lossd,Nuersailike Abuduwali,Rainer Meyer,Sied Kebir,Werner G,Andreas J
      Tissue engineering. Part A 18 2012

      显示摘要
      22497226
      Cell-derived matrix coatings for polymeric scaffolds.
      Martin L Decaris,Bernard Y Binder,Matthew A Soicher,Archana Bhat,J Kent Leach
      Tissue engineering. Part A 18 2012

      显示摘要
      22651377
      Neuroblast survival depends on mature vascular network formation after mouse stroke: role of endothelial and smooth muscle progenitor cell co-administration.
      Lina R Nih,Nicolas Deroide,Carole Leré-Déan,Dominique Lerouet,Mathieu Soustrat,Bernard I Levy,Jean-Sébastien Silvestre,Tatiana Merkulova-Rainon,Marc Pocard,Isabelle Margaill,Nathalie Kubis
      The European journal of neuroscience 35 2012

      显示摘要
      22512253
      Activation of Multiple ERBB Family Receptors Mediates Glioblastoma Cancer Stem-like Cell Resistance to EGFR-Targeted Inhibition.
      Paul A Clark,Mari Iida,Daniel M Treisman,Haviryaji Kalluri,Sathyapriya Ezhilan,Michael Zorniak,Deric L Wheeler,John S Kuo
      Neoplasia (New York, N.Y.) 14 2012

      显示摘要
      22745588
      Increasing doublecortin expression promotes migration of human embryonic stem cell-derived neurons.
      Radmila Filipovic,Saranya Santhosh Kumar,Chris Fiondella,Joseph Loturco
      Stem cells (Dayton, Ohio) 30 2012

      显示摘要
      22753232
      Generation and hepatic differentiation of human iPS cells.
      Tetsuya Ishikawa,Keitaro Hagiwara,Takahiro Ochiya
      Methods in molecular biology (Clifton, N.J.) 826 2012

      显示摘要
      22167643
      Significant clinical, neuropathological and behavioural recovery from acute spinal cord trauma by transplantation of a well-defined somatic stem cell from human umbilical cord blood.
      Jessica Schira,Marcia Gasis,Veronica Estrada,Marion Hendricks,Christine Schmitz,Thorsten Trapp,Fabian Kruse,Gesine Kögler,Peter Wernet,Hans-Peter Hartung,Hans Werner Müller
      Brain : a journal of neurology 135 2012

      显示摘要
      21903726
      A novel in vivo model for the study of human breast cancer metastasis using primary breast tumor-initiating cells from patient biopsies.
      Carolyn G Marsden,Mary Jo Wright,Latonya Carrier,Krzysztof Moroz,Radhika Pochampally,Brian G Rowan
      BMC cancer 12 2012

      显示摘要
      22233382
      Integration potential of mouse and human bone marrow-derived mesenchymal stem cells.
      Maria Kuzma-Kuzniarska,Aleksandra Rak-Raszewska,Simon Kenny,David Edgar,Bettina Wilm,Cristina Fuente Mora,Jamie A Davies,Patricia Murray
      Differentiation; research in biological diversity 83 2012

      显示摘要
      22364880
      Recurrent genomic instability of chromosome 1q in neural derivatives of human embryonic stem cells.
      Christine Varela,Jérôme Alexandre Denis,Jérôme Polentes,Maxime Feyeux,Sophie Aubert,Benoite Champon,Geneviève Piétu,Marc Peschanski,Nathalie Lefort
      The Journal of clinical investigation 122 2012

      显示摘要
      22269325

      小册子

      标题
      Cellutions - The newsletter for Cell Biology Researchers Volume 3: 2011

      数据表

      标题
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation