07-158 | Anti-KDR/Flk-1/VEGFR2 Antibody

Immunohistochemistry Analysis:
Representative lot data.
Paraffin-embedded mouse embryo vascular endothelium tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution of Anti-KDR/Flk-1/VEGFR2. Reactivity was detected using the IHC-Select® Detection Kit (Cat. No. DAB050). Staining pattern appears to be restricted endothelial cells with no smooth muscle involvement.
Immunohistochemistry Analysis:
Representative lot data.
Paraffin-embedded mouse embryo v
07-158
200 µL  
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      Overview

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      Tableau de caractéristiques principal

      Espèces réactives Principales applications Hôte Format Type d'anticorps
      SourisImmunohistochimie, WBLapinPurifié(e) par affinitéAnticorps polyclonal
      Description
      Référence 07-158
      Nom de marque
      • Upstate
      Description Anti-KDR/Flk-1/VEGFR2 Antibody
      Informations générales The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines. The VEGF receptors stimulate the Ras-MAP kinase pathway, suggesting that it signals as a conventional receptor tyrosine kinase.
      Références bibliographiques
      Informations produit
      Format Purifié(e) par affinité
      Contrôle
      • PAE cells transfected with CSF/VEGFR2 chimera and treated with CSF
      Présentation Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      Application This KDR/Flk-1/VEGFR2 antibody is validated for use in WB & IHC for the detection of the KDR/Flk-1/VEGFR2 protein.
      Principales applications
      • Immunohistochimie
      • Western Blotting
      Notes opératoires Immunohistochemistry Analysis: 1:100 dilution from a representative lot detected KDR/Flk-1/VEGFR2 in mouse embryo vascular endothelium tissue.
      Informations biologiques
      Espèces d'immunogène GST-tagged recombinant protein corresponding to mouse KDR/Flk-1/VEGFR2 at the cytoplasmic domain.
      Épitope Cytoplasmic domain
      Hôte Lapin
      Spécificité This antibody recognizes KDR/Flk-1/VEGFR2 at the cytoplasmic domain.
      Espèces réactives Souris
      Species Reactivity Note Demonstrated to react with Mouse. Expected to react with Rat based on a 99% sequence homology and Human based on 92% sequence homology.
      Type d'anticorps Anticorps polyclonal
      Code Entrez Gene
      Symbole du gène
      • CD309
      • KDR
      • VEGFR-2
      • VEGFR2
      • FLK1
      • VEGFR
      Méthode de purification Affinity purified
      Code UniProt
      Résumé UniProt FUNCTION: Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

      SIZE: 1356 amino acids; 151527 Da

      SUBUNIT: Interacts with SHB upon VEGF activation. Interacts with HIV-1 Tat.

      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

      SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 7 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
      Informations physico-chimiques
      Dimensions
      Informations sur les matériaux
      Informations toxicologiques
      Informations de sécurité selon le SGH
      Informations sur la sécurité
      Notifications sur l'utilisation du produit
      Assurance Qualité Evaluated by Western Blot in PAE cells transfected with CSF/VEGFR2 chimera and treated with CSF.

      Western Blot Analysis: 1:1,000 dilution of this antibody detected KDR/Flk-1/VEGFR2 on 10 µg of PAE cells transfected with CSF/VEGFR2 chimera and treated with CSF.
      Notification sur l'utilisation
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Informations relatives au stockage et à l'expédition
      Conditions de stockage Stable for 1 year at 2-8°C from date of receipt.
      Informations sur l'emballage
      Quantité 200 µL
      Informations sur le transport
      Information complémentaire
      Caractéristiques

      Documentation

      Certificats d'analyse

      TitreNuméro de lot
      Anti-KDR/Flk-1/VEGFR2 - 21522232152223
      Anti-KDR/Flk-1/VEGFR2 - 20154122015412
      Anti-KDR/Flk-1/VEGFR2 - 2026920269
      Anti-KDR/Flk-1/VEGFR2 - 22505172250517
      Anti-KDR/Flk-1/VEGFR2 - 23180932318093
      Anti-KDR/Flk-1/VEGFR2 - 2488924889
      Anti-KDR/Flk-1/VEGFR2 - 2970529705
      Anti-KDR/Flk-1/VEGFR2 - 3145931459
      Anti-KDR/Flk-1/VEGFR2 - NG1901588NG1901588
      Anti-KDR/Flk-1/VEGFR2 -25386252538625
      Anti-KDR/Flk-1/VEGFR2 -25943972594397

      Références bibliographiques

      Aperçu de la référence bibliographiqueNº PubMed
      Calsperin is a testis-specific chaperone required for sperm fertility.
      Ikawa M, Tokuhiro K, Yamaguchi R, Benham AM, Tamura T, Wada I, Satouh Y, Inoue N, Okabe M
      J Biol Chem 286 5639-46. Epub 2010 Dec 3. 2011

      Afficher le résumé Article en texte intégral
      21131354
      Compartmentalizing VEGF-induced ERK2/1 signaling in placental artery endothelial cell caveolae: a paradoxical role of caveolin-1 in placental angiogenesis in vitro.
      Wu-Xiang Liao, Lin Feng, Honghai Zhang, Jing Zheng, Thomas R Moore, Dong-Bao Chen, Wu-Xiang Liao, Lin Feng, Honghai Zhang, Jing Zheng, Thomas R Moore, Dong-Bao Chen
      Molecular endocrinology (Baltimore, Md.) 23 1428-44 2009

      Afficher le résumé Article en texte intégral
      19477952
      Receptor chimeras indicate that the vascular endothelial growth factor receptor-1 (VEGFR-1) modulates mitogenic activity of VEGFR-2 in endothelial cells.
      Rahimi, N, et al.
      J. Biol. Chem., 275: 16986-92 (2000) 2000

      Afficher le résumé
      10747927
      Vascular endothelial growth factor and hepatocyte growth factor levels are differentially elevated in patients with advanced retinopathy of prematurity.
      Lashkari, K, et al.
      Am. J. Pathol., 156: 1337-44 (2000) 2000

      Afficher le résumé
      10751359
      A role for cadherin-5 in regulation of vascular endothelial growth factor receptor 2 activity in endothelial cells.
      Rahimi, N and Kazlauskas, A
      Mol. Biol. Cell, 10: 3401-7 (1999) 1999

      Afficher le résumé
      10512875